FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691707

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691707
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1252736
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCGA68680.5482400122611628No Hit
CTCCTGCACTGGGAGCACCTCCAACATCGGGGCAGCTTATGATGTACACTGGTACCAGGAGCTTCCAGGAACAGC64790.5171879789516707No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC63790.5092054511086135No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCGAAGCACGCAGG61140.4880517523245121No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT53670.42842226933687544No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG53270.4252292581996526No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG49770.3972904107489527No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC49060.3916228159803821No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT43890.35035314703177683No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC42520.3394170838867886No Hit
GCTTATGATGTACACTGGTACCAGGAGCTTCCAGGAACAGCCCCCAAACTCCTCATCTATGGTACCAGCAATCGG36580.29200086849902934No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG36150.28856838152651476No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC36120.28832890569122305No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT36010.2874508276284868No Hit
GATGTACACTGGTACCAGGAGCTTCCAGGAACAGCCCCCAAACTCCTCAT35040.2797077756207214No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG34450.27499808419331767No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC31920.2548022887503832No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG31780.25368473485235515No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC31630.25248735567589664No Hit
GGCCATCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCATT31430.2508908501072852No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG31280.24969347093082658No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT30950.2470592367426178No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG30500.24346709921324203No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA30360.24234954531521408No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA29950.23907670889956062No Hit
GTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC29580.23612317359762952No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG29330.23412754163686522No Hit
GATTATTACTGCCATTCGTATGACAGCAGCCTGCGTGCTTCGGTATTCGG29110.23237138551139266No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT28680.22893889853887808No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCGAAGCACGCAGGC28220.22526693573107184No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA28220.22526693573107184No Hit
TCCAGGAACAGCCCCCAAACTCCTCATCTATGGTACCAGCAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGG28130.22454850822519667No Hit
CCTCCAACATCGGGGCAGCTTATGATGTACACTGGTACCAGGAGCTTCCA27290.2178431848370287No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC26870.2144905231429447No Hit
GGGCAGAGGGTCACCATCTCCTGCACTGGGAGCACCTCCAACATCGGGGC25260.20163865331562278No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT24730.1974079135588025No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGG24630.19660966077449676No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT24540.19589123326862165No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCGAAG24320.1941350771431491No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA24290.19389560130785735No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA24260.19365612547256564No Hit
GGATGAGGCTGATTATTACTGCCATTCGTATGACAGCAGCCTGCGTGCTTCGGTATTCGGCGGAGGGACCAAG23470.18734992847655052No Hit
TCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCATTCGTAT23020.18375779094717484No Hit
GTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAC22130.176653341166854No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCACTGGGCTCCAGGCTGAGGATG21830.17425858281393686No Hit
GCCCCCAAACTCCTCATCTATGGTACCAGCAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGT21520.17178399918258916No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC21430.17106557167671402No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT21370.17058662000613056No Hit
GCCTCATCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGCC21110.16851116276693573No Hit
GTGCAGGAGATGGTGACCCTCTGCCCTGGGGCCCCAGACACTGAGGGCGG20820.16619622969244915No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC20410.16292339327679572No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT20180.16108741187289263No Hit
GCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCC19830.15829352712782263No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG19800.15805405129253092No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG19690.15717597322979462No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG19570.15621806988862777No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA19300.15406278737100235No Hit
GTCATACGAATGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAG19160.15294523347297437No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC18940.15118907734750178No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC18930.15110925206907122No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC18920.15102942679064063No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC18660.1489539695514458No Hit
GCTTGGTCCCTCCGCCGAATACCGAAGCACGCAGGCTGCTGTCATACGAATGGCAGTAATAATCAGCCTCATCCT18580.14831536732400122No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC18460.1473574639828344No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT18410.14695833759068153No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTC18200.14528200674363953No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC18160.14496270562991723No Hit
GGCTGCTGTCATACGAATGGCAGTAATAATCAGCCTCATCCTCAGCCTGG17990.14360567589659753No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG17960.1433662000613058No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC17570.14025301420251352No Hit
AGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGT17410.13897580974762438No Hit
ACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGC17070.13626175028098497No Hit
TCGTATGACAGCAGCCTGCGTGCTTCGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG16850.13450559415551241No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG16660.13298891386533157No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC16650.13290908858690098No Hit
TCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACC16630.13274943803003983No Hit
GCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCATTCGTATGACAGCA16610.1325897874731787No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG16480.1315520588535813No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC16260.1297959027281087No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG16160.128997649943803No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC16050.1281195718810667No Hit
GCTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCT16040.12803974660263615No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16010.12780027076734446No Hit
GTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGATT16010.12780027076734446No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT15840.1264432410340247No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA15600.12452743435169102No Hit
CACTGGTACCAGGAGCTTCCAGGAACAGCCCCCAAACTCCTCATCTATGGTACCAGCAATCGGCCCTCAGGGGTC15510.12380900684581587No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT15310.12221250127720445No Hit
GCCCAGTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAAT15230.12157389904975989No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA15200.12133442321446816No Hit
CCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGG15080.12037651987330132No Hit
ACAGCAGCCTGCGTGCTTCGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCT15080.12037651987330132No Hit
GTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCACCT15040.12005721875957903No Hit
GTTTGGGGGCTGTTCCTGGAAGCTCCTGGTACCAGTGTACATCATAAGCT15020.1198975682027179No Hit
GTTCCTGGAAGCTCCTGGTACCAGTGTACATCATAAGCTGCCCCGATGTT14910.1190194901399816No Hit
CTCCTCATCTATGGTACCAGCAATCGGCCCTCAGGGGTCCCTGACCGATT14850.11854053846939817No Hit
GTGTACATCATAAGCTGCCCCGATGTTGGAGGTGCTCCCAGTGCAGGAGA14740.1176624604066619No Hit
GGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG14710.1174229845713702No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC14590.11646508123020333No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC14550.11614578011648105No Hit
GGCTGAGGATGAGGCTGATTATTACTGCCATTCGTATGACAGCAGCCTGCGTGCTTCGGTATTCGGCGGAGGGAC14420.11510805149688363No Hit
GTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGC14280.11399049759885563No Hit
CTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGTCTGTG14240.11367119648513335No Hit
GTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCC14120.11271329314396648No Hit
GGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCC13930.11119661285378564No Hit
GGGTCACCATCTCCTGCACTGGGAGCACCTCCAACATCGGGGCAGCTTATGATGTACACTGGTACCAGGAGCTTC13780.10999923367732707No Hit
CCCCTGAGGGCCGATTGCTGGTACCATAGATGAGGAGTTTGGGGGCTGTTCCTGGAAGCTCCTGGTACCAGTGTA13650.10896150505772964No Hit
GTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTT13510.10784395115970165No Hit
CCTCAGCCTCCCTGGCCATCACTGGGCTCCAGGCTGAGGATGAGGCTGAT13440.10728517421068764No Hit
GTCAGGGACCCCTGAGGGCCGATTGCTGGTACCATAGATGAGGAGTTTGGGGGCTGTTCCTGGAAGCTCCTGGTA13410.10704569837539593No Hit
GAATATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC13170.10512989169306224No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCACTGGGCTCCAGG12970.1035333861244508No Hit
CATCTATGGTACCAGCAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTC12930.10321408501072851No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG12830.10241583222642281No Hit
GCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAA12830.10241583222642281No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAC12770.10193688055583937No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGG12700.10137810360682539No Hit
GATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG12700.10137810360682539No Hit
GGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGG12590.10050002554408909No Hit
CCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGG12590.10050002554408909No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCCG252.287584E-456.50383461
GGTATCA11050.049.384581
CGTATGC850.048.89206341
TTATAGC405.168178E-542.88720722
ACGAGAC1150.041.7684122
ATGCCGT1000.041.65728844
GTATCAA33700.041.5515021
CGCAAAT350.001235819840.13068856
CACGAGA1250.038.4269421
CCCACGA1250.038.42387419
ATTATAG451.03476326E-438.12196721
GTGGTAT2300.037.3051381
TATCAAC37350.037.2937362
ATCTCCG1500.036.59270510
TCTCGTA1051.6370905E-1136.16734738
TATACAC1450.035.4914473
ATCAACG39300.035.4432833
TACCCGA400.00229640935.3744962
TCAACGC39700.035.086174
GCCGTCT1300.034.77480346
Produced by FastQC (version 0.11.5)