FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691708

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691708
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1252736
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGCACTGGGAGCACCTCCAACATCGGGGCAGCTTATGATGTACACTGGTACCAGGAGCTTCCAGGAACAGC62950.5025001277204455No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC60170.4803087003167467No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCGA59320.47352355165014814No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG56840.45372688259936644No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT50000.39912639215285584No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCGAAGCACGCAGG48390.38627452232553383No Hit
GTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTG46330.36983051496883623No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG42650.34045481250638604No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC41110.3281617196280781No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC40870.32624591294574434No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT40420.32265377541636864No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG39320.3138729947890058No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG38190.3048527383263513No Hit
GCTTATGATGTACACTGGTACCAGGAGCTTCCAGGAACAGCCCCCAAACTCCTCATCTATGGTACCAGCAATCGG35660.28465694288341675No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT34790.27771214365995706No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC33590.26813311024828856No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG33070.26398219576989884No Hit
GATGTACACTGGTACCAGGAGCTTCCAGGAACAGCCCCCAAACTCCTCAT32720.26118831102482887No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC31360.25033207315827116No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC30740.24538290589557576No Hit
GATTATTACTGCCATTCGTATGACAGCAGCCTGCGTGCTTCGGTATTCGG29800.23787932972310208No Hit
GGCCATCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCATT29770.23763985388781037No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA29610.23636264943292123No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG28820.2300564524369061No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG28150.22470815878205785No Hit
GTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCAC27710.22119584653111268No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA27700.22111602125268215No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT27410.21880108817819557No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG27380.21856161234290386No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA26990.2154484264841116No Hit
TCCAGGAACAGCCCCCAAACTCCTCATCTATGGTACCAGCAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGG26890.21465017369980588No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA26630.21257471646061102No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT26200.20914222948809646No Hit
CCTCCAACATCGGGGCAGCTTATGATGTACACTGGTACCAGGAGCTTCCA25890.20666764585674874No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT25820.20610886890773478No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT25770.20570974251558188No Hit
CTGCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCAC25160.20084040053131705No Hit
GTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC24480.19541228159803822No Hit
GGATGAGGCTGATTATTACTGCCATTCGTATGACAGCAGCCTGCGTGCTTCGGTATTCGGCGGAGGGACCAAG23960.19126136711964853No Hit
GGGCAGAGGGTCACCATCTCCTGCACTGGGAGCACCTCCAACATCGGGGC23910.19086224072749566No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC23260.18567359762950852No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCGAAGCACGCAGGC23250.18559377235107796No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT22770.18176215898641054No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC21990.175535787268826No Hit
GTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAC21400.17082609584142228No Hit
TCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCATTCGTAT21360.1705067947277No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA21130.16867081332379688No Hit
GCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCT21130.16867081332379688No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCACTGGGCTCCAGGCTGAGGATG20770.16579710330029632No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCGAAG20110.16052863492387862No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGG20100.16044880964544805No Hit
GCCCCCAAACTCCTCATCTATGGTACCAGCAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGT19960.15933125574742005No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG19460.15533999182589148No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC19330.15430226320629406No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG19230.15350401042198836No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC19230.15350401042198836No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT19090.15238645652396035No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC18510.14775659037498723No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC18480.14751711453969552No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTC18470.14743728926126495No Hit
AGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGT18240.1456013078573618No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT17900.1428872483907224No Hit
GTGCAGGAGATGGTGACCCTCTGCCCTGGGGCCCCAGACACTGAGGGCGG17730.14153021865740267No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC17690.1412109175436804No Hit
ACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGC17470.13945476141820784No Hit
GGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGC17260.13777843057116584No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC17100.13650122611627669No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA16820.1342661183202207No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC16720.133467865535915No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG16460.13139240829672014No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG16390.13083363134770615No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC16360.13059415551241443No Hit
GGCTGCTGTCATACGAATGGCAGTAATAATCAGCCTCATCCTCAGCCTGG16250.12971607744967814No Hit
TCGTATGACAGCAGCCTGCGTGCTTCGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG16120.12867834883008072No Hit
GCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCC16110.12859852355165013No Hit
GTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAC16020.12788009604577502No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG16000.12772044548891387No Hit
GTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCACCT15760.12580463880658016No Hit
GCTTGGTCCCTCCGCCGAATACCGAAGCACGCAGGCTGCTGTCATACGAATGGCAGTAATAATCAGCCTCATCCT15750.1257248135281496No Hit
GGGTCACCATCTCCTGCACTGGGAGCACCTCCAACATCGGGGCAGCTTATGATGTACACTGGTACCAGGAGCTTC15680.12516603657913558No Hit
GTCATACGAATGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAG15590.12444760907326044No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT15470.1234897057320936No Hit
GGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTC15430.1231704046183713No Hit
GGCTGAGGATGAGGCTGATTATTACTGCCATTCGTATGACAGCAGCCTGCGTGCTTCGGTATTCGGCGGAGGGAC15420.12309057933994073No Hit
GCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCATTCGTATGACAGCA15400.1229309287830796No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC15360.12261162766935732No Hit
GCCTCATCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGCC15300.12213267599877388No Hit
CTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGTCTGTG15270.12189320016348217No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG15150.12093529682231532No Hit
GCTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCT15060.12021686931644017No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC15010.11981774292428732No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA14990.11965809236742617No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14910.1190194901399816No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA14850.11854053846939817No Hit
CTCCTCATCTATGGTACCAGCAATCGGCCCTCAGGGGTCCCTGACCGATT14730.11758263512823132No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG14720.11750280984980076No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC14610.11662473178706448No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC14330.11438962399100848No Hit
GGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG14280.11399049759885563No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAC14220.1135115459282722No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAAC14200.11335189537141105No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT14090.11247381730867477No Hit
ACAGCAGCCTGCGTGCTTCGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCT14040.11207469091652192No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC13930.11119661285378564No Hit
GTATCAACGCAGAGTACGGGAGGCTCTGCTTCAGCTGTGGGCACAAGAGG13860.11063783590477164No Hit
CACTGGTACCAGGAGCTTCCAGGAACAGCCCCCAAACTCCTCATCTATGGTACCAGCAATCGGCCCTCAGGGGTC13770.10991940839889651No Hit
GTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGC13760.10983958312046592No Hit
GAATATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC13600.10856237866557679No Hit
GTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCC13460.1074448247675488No Hit
CCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGG13120.10473076530090938No Hit
GTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGATT13080.10441146418718708No Hit
CTCACTGCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCA12980.10361321140288136No Hit
GGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCC12800.10217635639113108No Hit
GATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG12680.10121845304996424No Hit
GTGTACATCATAAGCTGCCCCGATGTTGGAGGTGCTCCCAGTGCAGGAGA12570.10034037498722796No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACTC150.002424074368.5031363
GGTATCA11400.043.8853231
GTGGTAT3250.043.2286641
GTATCAA33600.041.609471
TGGTATC3600.038.0633772
TATCAAC36750.037.752662
ATCAACG37800.036.6981053
TCAACGC38300.036.211794
CAACGCA38750.035.9694485
AACGCAG39050.035.9562076
ACGCAGA41150.033.8715937
ACGGGTA1155.0931703E-1132.7636816
CGCAGAG42450.032.7536288
CAGAGTA43550.031.84514410
AGAGTAC43500.031.8055711
CGGGTAC652.4359215E-531.62314817
GCAGAGT44100.031.602029
CCCGAAA952.1634696E-730.38481767
CCGAAAC1002.2161475E-730.3012768
TAACGGC802.7655915E-630.18230836
Produced by FastQC (version 0.11.5)