FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004691709

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691709
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences139433
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT15561.115948161482576No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4590.32919036383065703No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3900.279704230705787No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3520.25245099797035137No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3330.23882438160263356No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3110.22304619422948657No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCC2680.1922070098183357No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2650.19005543881290657No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2560.18360072579661915No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2550.18288353546147612No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2420.17356006110461655No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2190.15706468339632657No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2180.1563474930611835No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2120.15204435105032527No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2100.15060997038003915No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2040.1463068283691809No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2000.1434380670286087No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1930.13841773468260743No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1920.13770054434746437No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1870.13411459267174916No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCG1850.13268021200146307No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1670.1197707859688883No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1640.11761921496345916No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1640.11761921496345916No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1540.11044731161202871No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1530.10973012127688568No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1520.10901293094174264No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1490.1068613599363135No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1450.10399259859574132No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1400.1004066469200261No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1400.1004066469200261No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)