FastQCFastQC Report
Wed 8 May 2024
EGAF00004691711

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691711
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1091776
Total Bases78.2 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGCTCCTCATATTCGAGCATCAGTACCCTTGTGATATTCGG35050.3210365496219004No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATATCAC33310.3050992144908846No Hit
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATATCACAAGGGTACTG32880.30116067764816223No Hit
GTAATAATCAGCCTCGTCGTCAGTCTGGAGCCCAGAGATGGACAGGGAGG32840.30079430212790903No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC32560.2982296734861363No Hit
GAGATGGACAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATT31370.2873300017586025No Hit
GTCCATCTCTGGGCTCCAGACTGACGACGAGGCTGATTATTACTGCTCCT29530.27047672782695353No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATCAGT27790.2545393926959376No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC27730.25398982941555776No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTT26990.24721188229087288No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTC26330.2411666862066944No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC24760.2267864470367548No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG24390.22339747347441233No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATGCATATGTCTCCT23450.2147876487484612No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT22760.20846767102409286No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC22650.20746013834339644No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA22290.2041627586611173No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA20960.1919807726126971No Hit
CCCCTAAGGGCCGATTAGTGACATCATAAATCATGAGTTTGGGGGCCTTG20510.18785904800984818No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA20410.18694310920921506No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT19990.18309616624655609No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC19330.17705097016237764No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGT19100.1749443109209215No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATG19060.17457793540066827No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC18690.1711889618383258No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA18560.1699982413975028No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT18460.16908230259686968No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTG18410.16862433319655315No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT18280.1674336127557301No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG18270.1673420188756668No Hit
ACACTGGTGTGTCTCATCAGTGACTTCTACCCGGGAGCCGTGACAGTGGC17670.16184638607186821No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT17570.16093044727123512No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA17350.1589153819098423No Hit
GGTGTGTCTCATCAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA17180.15735828594876605No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA17110.1567171287883229No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16970.15543481446743654No Hit
TCAGTACCCTTGTGATATTCGGCGGAGGGACCAAGGTGACCGTCCTAGGT16790.15378612462629696No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT16330.14957280614338472No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT16310.14938961838325812No Hit
GTCCAGGAGACCCAGACACGGAGGCAGGCTGAGTCAGGGCAGACTGGGCC15790.144626736619966No Hit
ACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATATCACAAGGGTACTGA15630.14316123453895305No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT15230.13949747933642065No Hit
CTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCG15160.1388563221759775No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGT15130.13858154053578756No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT15100.13830675889559765No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACC14970.1371160384547746No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTGA14920.13665806905445804No Hit
GGGTACTGATGCTCGAATATGAGGAGCAGTAATAATCAGCCTCGTCGTCA14790.13546734861363505No Hit
CCTTGGTCCCTCCGCCGAATATCACAAGGGTACTGATGCTCGAATATGAG14680.13445981593293863No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC14360.13152881177091272No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCA14320.1311624362506595No Hit
GTCTGGAGCCCAGAGATGGACAGGGAGGCCGTGTTGCCAGACTTGGAGCC14110.12923896476932997No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC14070.12887258924907674No Hit
GTGCAGGAGATGGTGATCGACTGTCCAGGAGACCCAGACACGGAGGCAGG14060.1287809953690134No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT13920.1274986810481271No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAA13800.12639955448736737No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCT13330.1220946421243918No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC13150.12044595228325225No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT13140.12035435840318894No Hit
TCTCTGGGCTCCAGACTGACGACGAGGCTGATTATTACTGCTCCTCATAT13060.11962160736268246No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGA13050.11953001348261914No Hit
GGGTAGAAGTCACTGATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG12950.11861407468198605No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA12900.1181561052816695No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGG12830.11751494812122633No Hit
CAACAACACCCAGGCAAGGCCCCCAAACTCATGATTTATGATGTCACTAA12770.11696538484084647No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGT12730.11659900932059324No Hit
AATATGAGGAGCAGTAATAATCAGCCTCGTCGTCAGTCTGGAGCCCAGAG12520.11467553783926372No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTC12370.11330162963831408No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG12260.11229409695761768No Hit
ATGCATAACCACCAACGTCACTGCTGGTTCCAGTGCAGGAGATGGTGATC12210.11183612755730113No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT12140.11119497039685797No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC12030.11018743771616156No Hit
CGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCAT12000.10991265607597162No Hit
CCTCATATTCGAGCATCAGTACCCTTGTGATATTCGGCGGAGGGACCAAG11910.10908831115540184No Hit
GTGTTGTTGGTACCAGGAGACATATGCATAACCACCAACGTCACTGCTGG11820.10826396623483206No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG11760.1077144029544522No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC11750.10762280907438888No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCG11670.1068900580338824No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGC11660.10679846415381909No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT11660.10679846415381909No Hit
AGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTC11570.10597411923324931No Hit
GTTATGCATATGTCTCCTGGTACCAACAACACCCAGGCAAGGCCCCCAAA11490.10524136819274282No Hit
GAATATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTT11410.10450861715223636No Hit
GGACAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATTAGAAA11390.10432542939210973No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTGATGAGACACAC11350.1039590538718565No Hit
AGGCCACACTGGTGTGTCTCATCAGTGACTTCTACCCGGGAGCCGTGACA11310.10359267835160325No Hit
ACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGG11310.10359267835160325No Hit
GCTCCAGACTGACGACGAGGCTGATTATTACTGCTCCTCATATTCGAGCA11050.10121123746995721No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA11040.10111964358989389No Hit
CCTTAGGGGTTTCTAATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCC10950.10029529866932411No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)