FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691712

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691712
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1091776
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG55660.5098115364323818No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC28600.2619584969810657No Hit
GATTATTACTGCTCCTCATATTCGAGCATCAGTACCCTTGTGATATTCGG27850.2550889559763175No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATATCAC27250.2495933231725189No Hit
GTCCATCTCTGGGCTCCAGACTGACGACGAGGCTGATTATTACTGCTCCT24870.2277939797174512No Hit
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATATCACAAGGGTACTG24670.225962102116185No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATCAGT24620.22550413271586844No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG24580.22513775719561524No Hit
GTAATAATCAGCCTCGTCGTCAGTCTGGAGCCCAGAGATGGACAGGGAGG24190.22156559587314614No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG24110.22083284483263968No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG24070.22046646931238642No Hit
GAGATGGACAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATT23120.211765050706372No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTT23070.21130708130605547No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC21180.19399583797408992No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC21130.19353786857377336No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATGCATATGTCTCCTGGTACCAACAACACCCAGGCAAGGC20330.1862103581687086No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG19300.1767761885221877No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT17860.1635866697930711No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17850.1634950759130078No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTC17560.16083885339117182No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT16990.15561800222756314No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC16890.15470206342693005No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATCAGTGACTTCTACC16830.15415250014655021No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATGCATATGTCTCCTGGTACCAACAACA16600.15204584090509407No Hit
CCCCTAAGGGCCGATTAGTGACATCATAAATCATGAGTTTGGGGGCCTTGCCTGGGTGTTGTTGGTACCAGGAGA16440.15058033882408112No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT16340.14966440002344802No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC16110.1475577407819919No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA16100.1474661469019286No Hit
GGTGTGTCTCATCAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA15680.14361920393926958No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG15520.14215370185825663No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15510.14206210797819335No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC15220.13940588545635735No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC15180.13903950993610412No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA14910.13656647517439474No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG14370.13162040565097602No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG13810.1264911483674307No Hit
TCAGTACCCTTGTGATATTCGGCGGAGGGACCAAGGTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA13770.12612477284717746No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT13610.1246592707661645No Hit
GTCTGGAGCCCAGAGATGGACAGGGAGGCCGTGTTGCCAGACTTGGAGCC13520.1238349258455947No Hit
ACACTGGTGTGTCTCATCAGTGACTTCTACCCGGGAGCCGTGACAGTGGC13360.12236942376458175No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC12780.11705697872090978No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT12670.11604944604021337No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT12660.11595785216015006No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTGATGAGACACACCAGTGTGGC12450.11403438067882057No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT12400.11357641127850403No Hit
ACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATATCACAAGGGTACTGA12140.11119497039685797No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT12110.11092018875666802No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC11940.10936309279559178No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT11870.1087219356351486No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC11810.10817237235476874No Hit
GGGTAGAAGTCACTGATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG11800.10808077847470543No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC11740.10753121519432558No Hit
CCTTGGTCCCTCCGCCGAATATCACAAGGGTACTGATGCTCGAATATGAGGAGCAGTAATAATCAGCCTCGTCGT11610.10634049475350255No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTGA11530.10560774371299608No Hit
GTGCAGGAGATGGTGATCGACTGTCCAGGAGACCCAGACACGGAGGCAGG11470.10505818043261622No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC11460.1049665865525529No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACC11460.1049665865525529No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG10940.1002037047892608No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA10920.10002051702913418No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTACG150.002857044365.720851
CGTGCTA150.002866407465.66662620
GTAAACT307.7414466E-444.13175628
TACGTAT1656.5923814E-834.78145670
CTATACA400.00331163432.8287964
GGTATCA20800.032.702441
GTATCAA45450.031.378271
CGTGTTC2450.031.20529252
GGGTAAG554.3324247E-429.8731121
ATTGTCT2400.029.23002464
CCCTAAA650.00598847529.08384569
AAGCGGT5500.028.69470270
CATTATG500.006412410628.6803449
TATCAAC50150.028.2857232
AGCAAGT550.00772501127.60674560
ACCGTGT2750.027.52547550
CGCAGAG52350.027.3429078
AACGCAG52950.027.2190786
AGAGTAC52300.027.17698911
ATCAACG52900.026.8724373
Produced by FastQC (version 0.11.5)