FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004691713

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691713
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences799745
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC52990.6625861993510431No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT44260.5534264046664875No Hit
ACATAAACCAGGGAAAGCCCCTAGGCTCCTGATATATGATTCATCCACTTTAGAATCTGACGTCCCGTCAAGGTT39090.4887807988796429No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC37110.46402290730170237No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC36110.45151892165627794No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA31730.3967514645293187No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC31360.3921249898405117No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG30560.3821218013241721No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG29450.3682423772577509No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG27780.34736072122989203No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC26370.3297301014698435No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT26280.32860474276175533No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26060.32585386591976195No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG23960.2995954960643705No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT23850.29822005764337384No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22740.28434063357695266No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22640.2830902350124102No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG21840.27308704649607063No Hit
CCCTTGGACGTTCGGCCAAGGGACCCAGGTGGAAATCAAACGAACTGTGG21640.2705862493669857No Hit
CTTTAGAATCTGACGTCCCGTCAAGGTTCAGTGGCAGTGGATCTGGGACAACTTTCACTCTCACCATCAGCAGT19540.24432787951159432No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19490.24370268022932312No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18890.2362002888420684No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTGGGTCCCTTGGCCGAACGTC18720.23407461128234622No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18460.23082357501453588No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG18310.22894797716772222No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17450.21819454951265715No Hit
GGTTTATGTTGATACCAATTTAAATAATTACTAATGGTCTGACTTGCCCG17440.21806950965620292No Hit
CTTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAATTTATTACTGTCAACAAAGTTACATGTCCC17310.21644399152229774No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17030.21294287554157887No Hit
CCCCTAGGCTCCTGATATATGATTCATCCACTTTAGAATCTGACGTCCCG17000.21256775597221614No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT16530.20669088271886663No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16470.20594064358014116No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA15980.19981369061388315No Hit
CCTGATATATGATTCATCCACTTTAGAATCTGACGTCCCGTCAAGGTTCAGTGGCAGTGGATCTGGGACAACTTT15870.1984382521928865No Hit
CACCATCAGCAGTCTGCAACCTGAAGATTTTGCAATTTATTACTGTCAACAAAGTTACATGTCCCCTTGGACGTT15850.198188172479978No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT15740.19681273405898128No Hit
ATTTATTACTGTCAACAAAGTTACATGTCCCCTTGGACGTTCGGCCAAGGGACCCAGGTGGAAATCAAACGAA15670.19593745506380159No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTGGGTCC15610.19518721592507612No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT15580.1948120963557134No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15340.19181113980081152No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15240.19056074123626907No Hit
GTTCAGTGGCAGTGGATCTGGGACAACTTTCACTCTCACCATCAGCAGTC15230.1904357013798148No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC15060.18831002382009265No Hit
GAGTGAAAGTTGTCCCAGATCCACTGCCACTGAACCTTGACGGGACGTCA15030.18793490425072992No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC14920.18655946582973323No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT14720.18405866870064833No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC14680.18355850927483136No Hit
GTCCAAGGGGACATGTAACTTTGTTGACAGTAATAAATTGCAAAATCTTC14580.1823081107102889No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14410.18018243315056673No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAATC14000.1750557990359427No Hit
GTCTGCAACCTGAAGATTTTGCAATTTATTACTGTCAACAAAGTTACATGTCCCCTTGGACGTTCGGCCAAGGGA13970.17468067946657997No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC13950.17443059975367148No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC13650.17067940406004414No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC13560.16955404535195592No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT13480.168553726500322No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13470.16842868664386773No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG13440.168053567074505No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA12780.15980093654852484No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG12730.15917573726625361No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA12710.15892565755334512No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG12670.15842549812752815No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT12260.15329886401290413No Hit
AAGTTACATGTCCCCTTGGACGTTCGGCCAAGGGACCCAGGTGGAAATCAAACGAACTGTGGCTGCACCAT12160.15204846544836167No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC11850.14817222989828008No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11710.14642167190792066No Hit
GTAATAAATTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAGTTGTCCCAGATCCACTGCCA11630.14542135305628667No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAATCTCCATCCCCT11570.14467111391756124No Hit
GCCAAGGGACCCAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTC11500.14379583492238152No Hit
CCATCAGCAGTCTGCAACCTGAAGATTTTGCAATTTATTACTGTCAACAA11470.14342071535301879No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT11400.14254543635783906No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11380.14229535664493057No Hit
ATATATGATTCATCCACTTTAGAATCTGACGTCCCGTCAAGGTTCAGTGGCAGTGGATCTGGGACAACTTTCACT11350.14192023707556783No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT11310.14142007764975087No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG11220.14029471894166265No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11110.13891928052066596No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11090.13866920080775746No Hit
GCCTAGGGGCTTTCCCTGGTTTATGTTGATACCAATTTAAATAATTACTA10740.1342928058318589No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC10710.13391768626249617No Hit
GTTACATGTCCCCTTGGACGTTCGGCCAAGGGACCCAGGTGGAAATCAAA10670.13341752683667918No Hit
CTGCTGATGGTGAGAGTGAAAGTTGTCCCAGATCCACTGCCACTGAACCT10210.12766569343978393No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA9960.1245396970284278No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA9920.12403953760261083No Hit
ATCATATATCAGGAGCCTAGGGGCTTTCCCTGGTTTATGTTGATACCAAT9850.12316425860743113No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA9780.12228897961225141No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT9710.12141370061707168No Hit
GGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCT9690.1211636209041632No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG9670.1209135411912547No Hit
GGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAG9630.12041338176543774No Hit
CCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAAC9620.12028834190898349No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC9340.11678722592826463No Hit
GCCCCTAGGCTCCTGATATATGATTCATCCACTTTAGAATCTGACGTCCCGTCAAGGTTCAGTGGCAGTGGAT9320.11653714621535614No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA9310.1164121063589019No Hit
GATATATGATTCATCCACTTTAGAATCTGACGTCCCGTCAAGGTTCAGTGGCAGTGGATCTGGGACAACTTTCA9250.11566186722017643No Hit
GCTGATGGTGAGAGTGAAAGTTGTCCCAGATCCACTGCCACTGAACCTTG9240.1155368273637222No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA9120.11403634908627126No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT9110.113911309229817No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC9080.11353618966045427No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC9050.11316107009109154No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT9010.11266091066527456No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT8930.1116605918136406No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC8750.10940987439746418No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC8530.10665899755547081No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG8490.10615883812965382No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC8480.10603379827319957No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC8450.10565867870383686No Hit
CTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAAT8330.1041582004263859No Hit
CCATATACGAGTGGGACCACAGCTGTGTAGCACTTGTTTCTGTCATAAGT8310.10390812071347742No Hit
ACTTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAATTTATTACTGTCAACAAAGTTACATGTCC8240.1030328417182977No Hit
ATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAG8160.10203252286666376No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA8050.10065708444566707No Hit
CTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTG8030.10040700473275856No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)