FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691714

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691714
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences799745
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT43650.5457989734227785No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC41430.5180401252899361No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC38480.48115336763593397No Hit
ACATAAACCAGGGAAAGCCCCTAGGCTCCTGATATATGATTCATCCACTTTAGAATCTGACGTCCCGTCAAGGTT37930.4742761755309505No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC37780.4724005776841368No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30940.38687331586943335No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA30180.37737028677891077No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC27920.3491112792202515No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27670.34598528280889534No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT27520.34410968496208166No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT27050.33823281170873215No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG25990.3249785869245822No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24150.30197125333700114No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG23860.29834509749982807No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22110.27646312262033523No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21900.2738372856347961No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21570.269710970371806No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT21070.26345897754909375No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19860.24832915491813015No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19820.2478289954923132No Hit
GGTTTATGTTGATACCAATTTAAATAATTACTAATGGTCTGACTTGCCCG19510.2439527599422316No Hit
CCCTTGGACGTTCGGCCAAGGGACCCAGGTGGAAATCAAACGAACTGTGG18740.23432469099525477No Hit
CTTTAGAATCTGACGTCCCGTCAAGGTTCAGTGGCAGTGGATCTGGGACAACTTTCACTCTCACCATCAGCAGT18570.2321990134355326No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTGGGTCCCTTGGCCGAACGTC17990.22494670176118636No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17940.22432150247891514No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17640.2205703067852878No Hit
CTTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAATTTATTACTGTCAACAAAGTTACATGTCCC17090.21369311468030436No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16950.2119425566899449No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA16820.21031703855603975No Hit
CCCCTAGGCTCCTGATATATGATTCATCCACTTTAGAATCTGACGTCCCG16590.20744112185759211No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG16290.20368992616396475No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16190.20243952759942233No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16170.20218944788651383No Hit
GTTCAGTGGCAGTGGATCTGGGACAACTTTCACTCTCACCATCAGCAGTC15980.19981369061388315No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15540.1943119369298964No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA15420.19281145865244548No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAATC15400.19256137893953698No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT15400.19256137893953698No Hit
GAGTGAAAGTTGTCCCAGATCCACTGCCACTGAACCTTGACGGGACGTCA15320.19156106008790302No Hit
CACCATCAGCAGTCTGCAACCTGAAGATTTTGCAATTTATTACTGTCAACAAAGTTACATGTCCCCTTGGACGTT15270.1909358608056318No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC15050.18818498396363842No Hit
CCTGATATATGATTCATCCACTTTAGAATCTGACGTCCCGTCAAGGTTCAGTGGCAGTGGATCTGGGACAACTTT14980.1873097049684587No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC14850.1856841868345535No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC14550.18193299114092615No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTGGGTCC14460.18080763243283798No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14440.1805575527199295No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT14380.179807313581204No Hit
ATTTATTACTGTCAACAAAGTTACATGTCCCCTTGGACGTTCGGCCAAGGGACCCAGGTGGAAATCAAACGAA14350.17943219401184127No Hit
GTCCAAGGGGACATGTAACTTTGTTGACAGTAATAAATTGCAAAATCTTC14030.17543091860530544No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14010.17518083889239694No Hit
GTCTGCAACCTGAAGATTTTGCAATTTATTACTGTCAACAAAGTTACATGTCCCCTTGGACGTTCGGCCAAGGGA13860.17330524104558329No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC13740.17180476276813234No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT13730.1716797229116781No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13560.16955404535195592No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT13340.16680316850996255No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC12940.16180157425179276No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT12500.15629982056780597No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12390.15492438214680931No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT12220.15279870458708714No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC12130.15167334587899892No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC11660.14579647262564943No Hit
ATATATGATTCATCCACTTTAGAATCTGACGTCCCGTCAAGGTTCAGTGGCAGTGGATCTGGGACAACTTTCACT11650.14567143276919517No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT11410.1426704762142933No Hit
AAGTTACATGTCCCCTTGGACGTTCGGCCAAGGGACCCAGGTGGAAATCAAACGAACTGTGGCTGCACCAT11360.14204527693202207No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA11320.1415451175062051No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11180.13979455951584568No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG11160.1395444798029372No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA11030.137918961669032No Hit
GTAATAAATTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAGTTGTCCCAGATCCACTGCCA11030.137918961669032No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT11030.137918961669032No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA10920.13654352324803531No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10900.13629344353512682No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT10780.13479296525767587No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG10690.1336676065495877No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAATCTCCATCCCCT10570.13216712827213675No Hit
CCATCAGCAGTCTGCAACCTGAAGATTTTGCAATTTATTACTGTCAACAA10410.1301664905688688No Hit
GCCTAGGGGCTTTCCCTGGTTTATGTTGATACCAATTTAAATAATTACTA10310.12891609200432638No Hit
CTGCTGATGGTGAGAGTGAAAGTTGTCCCAGATCCACTGCCACTGAACCT10090.126165215162333No Hit
GCCAAGGGACCCAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTC10050.125665055736516No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT9970.12466473688488207No Hit
GGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTG9950.12441465717197356No Hit
GTTACATGTCCCCTTGGACGTTCGGCCAAGGGACCCAGGTGGAAATCAAA9830.12291417889452262No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG9720.12153874047352592No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA9690.1211636209041632No Hit
ATCATATATCAGGAGCCTAGGGGCTTTCCCTGGTTTATGTTGATACCAAT9620.12028834190898349No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC9360.11703730564117311No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG9340.11678722592826463No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC9300.11628706650244766No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG9240.1155368273637222No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG9180.11478658822499671No Hit
GCCCCTAGGCTCCTGATATATGATTCATCCACTTTAGAATCTGACGTCCCGTCAAGGTTCAGTGGCAGTGGAT9080.11353618966045427No Hit
GCTGATGGTGAGAGTGAAAGTTGTCCCAGATCCACTGCCACTGAACCTTG9080.11353618966045427No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA9000.11253587080882033No Hit
GGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCT8980.11228579109591182No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC8850.11066027296200663No Hit
GGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAG8800.11003507367973542No Hit
ACTTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAATTTATTACTGTCAACAAAGTTACATGTCC8610.10765931640710476No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT8590.10740923669419628No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC8490.10615883812965382No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT8480.10603379827319957No Hit
GATATATGATTCATCCACTTTAGAATCTGACGTCCCGTCAAGGTTCAGTGGCAGTGGATCTGGGACAACTTTCA8460.10578371856029109No Hit
GTCTGCGTCTGTAGGCGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA8380.10478339970865713No Hit
ATTCTAAAGTGGATGAATCATATATCAGGAGCCTAGGGGCTTTCCCTGGT8290.10365804100056894No Hit
CCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAAC8230.10290780186184346No Hit
CAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTA8180.10228260257957225No Hit
ATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAG8120.10153236344084676No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT8090.10115724387148403No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA8080.1010322040150298No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT8040.10053204458921282No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG8030.10040700473275856No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC8000.10003188516339583No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGCGT200.00785347850.904321
GTGGTAT2050.041.349351
ATTCACT2100.035.5055052
GACAGGT1650.034.9339331
CTATTTA400.00282639233.9064671
TGTTAGA13400.033.281470
ATCAACG20900.033.2388153
TCAACGC21150.032.8438644
CAACGCA21250.032.5318835
GTGCTAG1057.730705E-1032.291871
GAGTTTA1800.032.022771
AACGCAG21750.031.7840256
ATACATC450.00420937231.25809551
TCACTTT2450.030.4275784
TCGCCTA11900.030.41573770
AATGGTA4700.030.25395270
GAGTTGC450.005006659330.1692922
AGAGTAC22950.029.97445511
CGCAGAG23000.029.9092948
GCAGAGT23050.029.8444149
Produced by FastQC (version 0.11.5)