FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691715

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691715
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1713273
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT84970.4959513165735992No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC70680.4125437101967988No Hit
GAGTTACAGTAGCCCTCGGACGTTCGGCCAGGGGACCAAGGTGGAGATCAGACGAACTGTGGCTGCACCAT68680.4008701473728939No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC52200.3046799897039176No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC52060.3038628403062443No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT46710.27263605975229865No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG45430.2651649795449995No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG43750.25535918677291947No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA42410.24753789968090315No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG41470.24205132515366787No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC40660.23732353220998637No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGATCGCTGATGGTGAGAGTGAAATCTGTC40560.23673985406879114No Hit
CCATTACATGCCGGGCAAGTCAGGCCATTAGTTATTTCTTACATTGGTATCAGCAGAAACCAGGGAAGGCCCCTA39360.22973571637444823No Hit
CTCCTGATCTCTGGTGCATCGACTTTGCATACTGGGGTCCCATCAAGGTT38370.2239573027766153No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT37840.22086380862828048No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG37840.22086380862828048No Hit
GTCTCCACCCTCCCTGTCTGCGTATGTTGGAGACAGCGTCACCATTACAT36390.21240047558094946No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG36090.21064944115736373No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT35350.20633022291251893No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC35080.20475429193129174No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG34400.20078528057116407No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG34310.20025997024408834No Hit
CCCCTACACTCCTGATCTCTGGTGCATCGACTTTGCATACTGGGGTCCCA34120.1991509817758174No Hit
GTGTAGGGGCCTTCCCTGGTTTCTGCTGATACCAATGTAAGAAATAACTA33020.1927305222226697No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT32540.18992886714493254No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT32530.18987049933081301No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT32300.18852803960606393No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT31760.1853761776436096No Hit
GTGCAGCCACAGTTCGTCTGATCTCCACCTTGGTCCCCTGGCCGAACGTC31720.1851427063871315No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC30790.17971449967401576No Hit
GTTGGAGACAGCGTCACCATTACATGCCGGGCAAGTCAGGCCATTAGTTATTTCTTACATTGGTATCAGCAGAAA30420.17755489055159335No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG29620.1728854654220314No Hit
GATTTCACTCTCACCATCAGCGATCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTAGC28790.16804093685011087No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATCTCCACCTTGGTCCCCTGGCCGAACGTCCGAGGGCTA28500.16634827024064466No Hit
ACTTACTACTGTCAACAGAGTTACAGTAGCCCTCGGACGTTCGGCCAGGGGACCAAGGTGGAGATCAGACGAA28010.16348824734878795No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGATCGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA26590.15520001774381548No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT26490.15461633960262025No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA26400.15409102927554452No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC25310.14772893753651636No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25100.14650321344000636No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG25080.1463864778117673No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT24830.14492728245877917No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC24360.14218399519516153No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA24290.14177542049632486No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG24220.1413668457974882No Hit
GATCAGGAGTGTAGGGGCCTTCCCTGGTTTCTGCTGATACCAATGTAAGA23510.13722273099500196No Hit
CACCATCAGCGATCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTAGCCCTCGGACGTT23000.13424597247490622No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA22900.133662294333711No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCACCCTCC22050.1287010301335514No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT21870.12765040947939996No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCA21670.12648305319700948No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC21370.12473201877342373No Hit
ATTACATGCCGGGCAAGTCAGGCCATTAGTTATTTCTTACATTGGTATCAGCAGAAACCAGGGAAGGCCCCTACA21050.12286424872159896No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT20720.12093811085565465No Hit
CCCTTAGACTCTCCTGTGCAGTCTCTGGGCTCACTTTCAATAGCGCCTGG20500.11965401894502511No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT20350.11877850173323225No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT20330.11866176610499318No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT19880.11603521446961458No Hit
CTACACTCCTGATCTCTGGTGCATCGACTTTGCATACTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGA19840.1158017432131365No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT19800.11556827195665839No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA19400.11323355939187742No Hit
CTCCTGTGCAGTCTCTGGGCTCACTTTCAATAGCGCCTGGATGAATTGGGTCCGCCAGGCTCCAGGGCAGGGG19360.11300008813539932No Hit
GTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCGATCTGCAACCTGAAGATTTTGCAACTTA19350.11294172032127979No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC19270.1124747778083236No Hit
GCCACAGTTCGTCTGATCTCCACCTTGGTCCCCTGGCCGAACGTCCGAGG19200.11206620310948692No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA19150.1117743640388893No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC18720.10926454803174976No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG18680.10903107677527166No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT18640.10879760551879357No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA18490.1079220883070007No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC18460.10774698486464211No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC18430.10757188142228355No Hit
CATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCGATCTGCAACCTGAAGATTTTG18420.10751351360816401No Hit
CTCCCTGTCTGCGTATGTTGGAGACAGCGTCACCATTACATGCCGGGCAA18100.10564574355633924No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA18060.10541227229986115No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG18030.10523716885750256No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT18020.10517880104338304No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA17770.10371960569039494No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA17770.10371960569039494No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC17700.10331103099155826No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC17680.1031942953633192No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC17590.10266898503624348No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC17350.1012681574973749No Hit
GCATACTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATT17320.10109305405501634No Hit
CTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGA17220.10050937591382109No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGTAC200.00604751254.3841457
TCGTATG5600.046.8189840
CTTATAC5850.046.3665471
TATACAC6000.045.749473
CGTATGC5900.045.14527541
ACGAGAC5950.043.9129622
CGAGACG5850.043.53364623
GACGGAC5900.043.23039226
TATCTCG5650.042.87329536
CTCCTAT5850.042.24596832
ACGTAGC350.001037039641.5880358
CACGAGA6300.041.4685121
ATCTCGT6100.040.93627537
TCCTATC5800.040.92791433
CCTATCT5900.040.89362734
ATGCCGT6500.040.76054444
TCTCCGA6550.040.37351611
CCCACGA6600.040.08169619
ATCTCCG6600.040.06648610
GTATGCC6700.039.8654142
Produced by FastQC (version 0.11.5)