FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691716

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691716
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1713273
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT84210.49151536270051527No Hit
GAGTTACAGTAGCCCTCGGACGTTCGGCCAGGGGACCAAGGTGGAGATCAGACGAACTGTGGCTGCACCAT65830.3842353203488294No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC60120.3509072984865809No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC55970.3266846556269783No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC51640.3014113921132242No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT48970.2858271857433112No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG45280.26428946233320666No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG44480.25962003720364474No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG42270.24672075028322982No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC42050.24543665837260029No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA39920.2330043139651416No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGATCGCTGATGGTGAGAGTGAAATCTGTC38610.22535813031548385No Hit
CTCCTGATCTCTGGTGCATCGACTTTGCATACTGGGGTCCCATCAAGGTT37400.21829562480702144No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT35630.2079645217078656No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT35540.20743921138078988No Hit
CCATTACATGCCGGGCAAGTCAGGCCATTAGTTATTTCTTACATTGGTATCAGCAGAAACCAGGGAAGGCCCCTA34840.20335346439242316No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT34620.20206937248179363No Hit
GTCTCCACCCTCCCTGTCTGCGTATGTTGGAGACAGCGTCACCATTACAT34370.20061017712880552No Hit
GTGTAGGGGCCTTCCCTGGTTTCTGCTGATACCAATGTAAGAAATAACTA33710.19675790139691687No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT33470.19535707385804832No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG33070.19302236129326733No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC32980.19249705096619163No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG32890.19197174063911587No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG32260.18829456834958586No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG32230.18811946490722728No Hit
GTGCAGCCACAGTTCGTCTGATCTCCACCTTGGTCCCCTGGCCGAACGTC32030.1869521086248368No Hit
CCCCTACACTCCTGATCTCTGGTGCATCGACTTTGCATACTGGGGTCCCA31610.18450066043181676No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT30540.17825530432102762No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG30290.17679610896803954No Hit
GTTGGAGACAGCGTCACCATTACATGCCGGGCAAGTCAGGCCATTAGTTATTTCTTACATTGGTATCAGCAGAAA30180.17615406301272477No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC30050.17539528142917094No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA29870.1743446607750195No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT28370.16558948865709083No Hit
GATTTCACTCTCACCATCAGCGATCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTAGC28330.16535601740061276No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGATCGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA27490.1604531210145727No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATCTCCACCTTGGTCCCCTGGCCGAACGTCCGAGGGCTA27130.15835187970626982No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT26530.15484981085909832No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG26070.1521648914096002No Hit
ACTTACTACTGTCAACAGAGTTACAGTAGCCCTCGGACGTTCGGCCAGGGGACCAAGGTGGAGATCAGACGAA25690.1499469144730583No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA25490.14877955819066782No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG24980.14580279967057205No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC24920.1454525927858549No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG24530.14317624803519344No Hit
GATCAGGAGTGTAGGGGCCTTCCCTGGTTTCTGCTGATACCAATGTAAGA23210.13547169657141622No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC22870.1334871908913524No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCA22490.13126921395481048No Hit
CACCATCAGCGATCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTAGCCCTCGGACGTT22460.1310941105124519No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT22190.12951817953122474No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA21650.12636631756877043No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21390.12484875440166279No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT21230.1239148693757504No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT21200.12373976593339181No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCACCCTCC21040.12280588090747943No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA20690.12076300741329607No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA20260.11825319140615653No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA19820.11568500758489746No Hit
ATTACATGCCGGGCAAGTCAGGCCATTAGTTATTTCTTACATTGGTATCAGCAGAAACCAGGGAAGGCCCCTACA19790.11550990414253887No Hit
CTCCTGTGCAGTCTCTGGGCTCACTTTCAATAGCGCCTGGATGAATTGGGTCCGCCAGGCTCCAGGGCAGGGG19770.11539316851429983No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT18890.11025680087178168No Hit
CTACACTCCTGATCTCTGGTGCATCGACTTTGCATACTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGA18810.10978985835882547No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC18740.10938128365998881No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC18640.10879760551879357No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT18460.10774698486464211No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT18230.10640452513989306No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC18170.1060543182551759No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC18140.10587921481281734No Hit
GTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCGATCTGCAACCTGAAGATTTTGCAACTTA18130.10582084699869782No Hit
CCCTTAGACTCTCCTGTGCAGTCTCTGGGCTCACTTTCAATAGCGCCTGG18070.10547064011398066No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC18040.10529553667162209No Hit
GGAGTGTAGGGGCCTTCCCTGGTTTCTGCTGATACCAATGTAAGAAATAA17780.10377797350451445No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC17450.10185183563857014No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT17380.10144326093973349No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA17360.10132652531149443No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC17310.10103468624089681No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAATT300.002445044268.35248670
AATGGTA10400.036.14795370
GTATCAA39300.035.760291
TATCAAC40950.033.979642
GTGGTAT4350.033.8460241
ATCAACG42900.032.3533633
TCAACGC43050.032.2396934
AACGCAG43500.032.060886
CAACGCA43500.031.9071085
TACGTAT1103.4413594E-431.06931370
CGCAGAG47000.029.6733678
AGAGTAC46700.029.64913711
GCAGAGT47350.029.3816789
TGTTAGA22150.028.0816170
CTATAGT500.008979795526.7691271
CGTCTAC801.17722986E-425.0960581
ACGGGGT3900.024.88010816
GATTCAC2850.024.6557751
GAGTACG36750.024.48211312
AGTACGG36800.024.35938513
Produced by FastQC (version 0.11.5)