FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691717

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691717
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1364741
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT59930.43913094132879427No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC53690.3934079799756877No Hit
GGGTGCACACTGGCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT40960.30013020785628924No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC39240.28752708389357395No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC38880.284889220738587No Hit
GTGTGCACCCTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACCC36780.26950168566783No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG35570.2606355345080129No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTA35270.25843731521219043No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG34220.25074354767681195No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA34070.24964443802890074No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC33240.24356269797712535No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT32400.23740768394882253No Hit
CGTATATAGTGATGGAAACACCTACTTGAATTGGTTTCAGCAGAGGCCAG31810.23308451933370505No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG29910.21916246379349635No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG28600.20956357286840507No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG28480.2086842851500761No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC26710.19571479130472375No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT26440.19373639393848358No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT26230.19219764043140786No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25800.18904685944072905No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24770.18149963985840536No Hit
GTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCACTGATTTCACACTGAA24240.17761611910245242No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACCCTACTAATTTTCAGTGTGAAATCAGTGCCTGACCCACTGCCG23270.17050854337929322No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC23150.16962925566096423No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC21810.15981054280629073No Hit
GGCCAGGCCAATCTCCAAGGCGCCTAATTTATAAGGTTTCTAACCGGGAC21630.15849161122879726No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG21420.15695285772172157No Hit
GTGCACACTGGCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA21380.1566597618156119No Hit
GTCTGGGACCCCAGAGTCCCGGTTAGAAACCTTATAAATTAGGCGCCTTGGAGATTGGCCTGGCCTCTGCTGAAA21050.15424172059020722No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20940.15343570684840566No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG20510.15028492585772685No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19680.14420318580595146No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC19610.14369026797025955No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18710.13709561008279225No Hit
CTATATACGAGGCTTTGACTAGACCTGCAGGAGATGGAGGCCGGCTGTCC18500.13555685657571656No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18340.13438447295127792No Hit
GTTTATTACTGCATGCAGGGTGCACACTGGCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA17940.13145351389018137No Hit
GGCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC17910.1312336919605991No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17670.12947511652394117No Hit
GCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGT17270.1265441574628446No Hit
GTCCAAGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAACATCC17060.1250054039557689No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT17030.12478558202618664No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16950.12419939021396734No Hit
GTCTCCACTCTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCT16820.12324682851911095No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC16800.12310028056605613No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT16770.12288045863647389No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA16590.12156152705898042No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA16570.1214149791059256No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA16540.12119515717634334No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16260.11914348583357574No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGTCTCCACTCTCC16040.11753145834997264No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC16000.11723836244386296No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT15970.11701854051428073No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA15680.11489359519498571No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT15620.11445395133582123No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC15380.11269537589916327No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG15220.11152299227472466No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGA14960.10961786888501188No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG14630.1071998276596072No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA14610.10705327970655237No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14560.10668690982391532No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC14460.10595417005864116No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC13960.10229047123227046No Hit
TTATTACTGCATGCAGGGTGCACACTGGCCGTGGACGTTCGGCCAAGGGA13900.10185082737310595No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG13800.10111808760783182No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG13780.10097153965477698No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC13750.10075171772519474No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13740.10067844374866733No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG2500.045.64871640
CGTATGC2650.043.11619641
GGTATCA12450.041.8505821
GTATCAA36350.040.5660671
CGAACTA702.401066E-839.0185829
CCGTCTT3150.038.79024547
ACGAGAC3050.037.9586522
GCCGTCT3200.037.03192546
ATCTCGT2900.036.7906437
CACGAGA3200.036.17801321
AATCTCG2950.036.13226336
CGAGACT3250.035.63187423
GCTAATC3000.035.42122333
TATGCCG3250.035.25616543
TATCAAC41850.034.7274632
CTTATAC3750.033.6008221
GTGGTAT4100.033.224281
ATCAACG45350.032.346313
TCAACGC45500.032.164874
CAACGCA45850.031.9193365
Produced by FastQC (version 0.11.5)