FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004691719

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691719
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences890949
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATGGGTAGTGGCATTGTGATCTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC46850.5258437912832272No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC40220.45142875742607036No Hit
CCCATATATAGCACACAGTAATAATCAGATTCATCATCTGCCTGGGCCCCCGTGATGGTGAGGGCAGCTTTGTTC38700.4343682971752592No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGATCACAATGCCACTACCCATATATAGCACA38080.42740942523084935No Hit
GATTATTACTGTGTGCTATATATGGGTAGTGGCATTGTGATCTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT38030.4268482258804937No Hit
GTAATAATCAGATTCATCATCTGCCTGGGCCCCCGTGATGGTGAGGGCAG37860.42494014808928454No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGATCACAATGCCACTACCCATATATAGCACACAGTAATAAT30810.34581103968914045No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT30600.3434540024176468No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG30120.3380664886542327No Hit
GTAATACATAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGAGA29640.33267897489081866No Hit
CTCATCTACAGCACAAACACTCGCTCTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCCTTGGGAACAAAG29610.3323422552806053No Hit
CACTTAGCCTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCT28470.31954691009249686No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC27020.3032721289321835No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG26630.2988947739994096No Hit
CTCTAGTAGTTACTACCCCAGCTGGTACCAGCAGACCCCAGGCCAGGCTCCACGCACGCTCATCTACAGCACAAA24590.27599784050489984No Hit
GGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATTCTCAAACTGTGGTGACCCAGGAGCCAT23830.26746761037949424No Hit
GTAGATGAGCGTGCGTGGAGCCTGGCCTGGGGTCTGCTGGTACCAGCTGG23390.2625290560963647No Hit
GTGCTATATATGGGTAGTGGCATTGTGATCTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG23020.258376180903733No Hit
ATGTATTACTGTGCGAGATGTTATAGGGGCTACGGTGACTACGTTGACTA22890.2569170625928084No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT22480.25231522791989214No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC22310.25040715012868303No Hit
CTACTAGAGACTGAGCCAGAGGTCAAGCCACAAGTGAGTGTGACCGTCCCTCCAGGGGACACTGAGAACGATGG22230.24950923116811402No Hit
ATATAGCACACAGTAATAATCAGATTCATCATCTGCCTGGGCCCCCGTGA21590.2423258794835619No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC21130.2371628454602901No Hit
GTCTCTGGTGGCTCCATCAGCGATAGTAGTTACTACTGGGGCTGGATCCG20280.22762245650424437No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT19870.22302062183132818No Hit
CTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCCTTGGGAACAAAGCTGCCCTCACCATCACGGGGGCCCAGG19770.2218982231306169No Hit
CCCCAGGCCAGGCTCCACGCACGCTCATCTACAGCACAAACACTCGCTCT19160.2150515910562782No Hit
GTCTCTAGTAGTTACTACCCCAGCTGGTACCAGCAGACCCCAGGCCAGGC19080.21415367209570915No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18370.20618464132065922No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG18240.20472552300973457No Hit
CCCCAGAAGAGCGAGTGTTTGTGCTGTAGATGAGCGTGCGTGGAGCCTGG18200.20427656352945003No Hit
GGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGG18090.20304192495866769No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG17840.2002359282068895No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA17710.19877680989596486No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC17510.19653201249454233No Hit
CTTGTGGCTTGACCTCTGGCTCAGTCTCTAGTAGTTACTACCCCAGCTGGTACCAGCAGACCCCAGGCCAGGCTC17320.19439945496319094No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG17280.1939504954829064No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC16760.18811402223920787No Hit
GATCAGGAGTGGATTCTCAAACTGTGGTGACCCAGGAGCCATCGTTCTCAGTGTCCCCTGGAGGGACGGTCACA16600.18631818431806985No Hit
CCCCTGGAGGGACGGTCACACTCACTTGTGGCTTGACCTCTGGCTCAGTC16430.18441010652686068No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT16270.18261426860572266No Hit
GCCTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACT16200.18182858951522476No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA16180.1816041097750825No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT16140.181155150294798No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15800.1773389947123797No Hit
CATCTACAGCACAAACACTCGCTCTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCCTTGGGAACAAAGCTGC15610.1752064371810283No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA15570.17475747770074382No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG15540.17442075809053043No Hit
CAGTAATACATAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGA15540.17442075809053043No Hit
CACTTGTGGCTTGACCTCTGGCTCAGTCTCTAGTAGTTACTACCCCAGCTGGTACCAGCAGACCCCAGGCCAGG15250.17116580185846778No Hit
ACTAGAGACTGAGCCAGAGGTCAAGCCACAAGTGAGTGTGACCGTCCCTCCAGGGGACACTGAGAACGATGGCTC15110.16959444367747198No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC14800.16611500770526708No Hit
CAGTAATAATCAGATTCATCATCTGCCTGGGCCCCCGTGATGGTGAGGGC14160.158931656020715No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGATCACAATGCCACTAC14120.15848269654043048No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCGATAGTAGTTACTACTGGG13980.15691133835943472No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13720.15399310173758543No Hit
GTATTACTGTGCGAGATGTTATAGGGGCTACGGTGACTACGTTGACTACTGGGGCCAGGGAACCCTGGTCACCGT13580.15242174355658966No Hit
GTGGTGACCCAGGAGCCATCGTTCTCAGTGTCCCCTGGAGGGACGGTCACACTCACTTGTGGCTTGACCTCTGG13290.14916678732452698No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT13030.1462485507026777No Hit
GGAGTGGATTCTCAAACTGTGGTGACCCAGGAGCCATCGTTCTCAGTGTC12970.14557511148225094No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT12940.14523839187203758No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC12850.14422823304139745No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA12780.14344255395089955No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC12720.1427691147304728No Hit
GGCCTGGGGTCTGCTGGTACCAGCTGGGGTAGTAACTACTAGAGACTGAG12390.1390651990181256No Hit
CACCATCACGGGGGCCCAGGCAGATGATGAATCTGATTATTACTGTGTGCTATATATGGGTAGTGGCATTGTGAT12320.13827951992762774No Hit
CTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATT12230.1372693610969876No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT12070.13547352317584957No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC11880.13334096564449818No Hit
ACCCATATATAGCACACAGTAATAATCAGATTCATCATCTGCCTGGGCCCCCGTGATGGTGAGGGCAGCTTTGTT11580.1299737695423644No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC11510.1291880904518665No Hit
GTGATGGTGAGGGCAGCTTTGTTCCCAAGGATGGAGCCAGAGAAGCGATC11430.12829017149129748No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGATCACAATGCCA11420.12817793162122637No Hit
GTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGG11300.12683105318037283No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG11240.12615761395994607No Hit
GTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATTCTCAAA11240.12615761395994607No Hit
CTACAACCCGTCCCTCAAGAGTCGAGTCTCCATATCCGTGGACACGTCCAAGAACCAATTCTCCCTGAAGCTGAG10990.12335161720816792No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC10890.12222921850745666No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA10860.12189249889724327No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT10820.12144353941695876No Hit
GCCTGGGCCCCCGTGATGGTGAGGGCAGCTTTGTTCCCAAGGATGGAGCC10750.12065786032646089No Hit
CTCCATATCCGTGGACACGTCCAAGAACCAATTCTCCCTGAAGCTGAGCT10650.11953546162574963No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG10630.11931098188560738No Hit
ACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATTCTCAAACTGTGGTGACC10550.11841306292503836No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT10530.1181885831848961No Hit
AGCCACAAGTGAGTGTGACCGTCCCTCCAGGGGACACTGAGAACGATGGCTCCTGGGTCACCACAGTTTGAGAAT10460.11740290409439821No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC10450.1172906642243271No Hit
CTATTATAGTGGGAGCACCCACTACAACCCGTCCCTCAAGAGTCGAGTCTCCATATCCGTGGACACGTCCAAGAA10430.11706618448418483No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG10380.1165049851338292No Hit
GCACAAACACTCGCTCTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATC10290.11549482630318908No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC10270.11527034656304681No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG10190.11437242760247782No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA10160.11403570799226442No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC10060.11291330929155316No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC10060.11291330929155316No Hit
TTATTACTGTGTGCTATATATGGGTAGTGGCATTGTGATCTTCGGCGGAG9920.11134195111055739No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC9880.11089299163027289No Hit
GCTTGGTCCCTCCGCCGAAGATCACAATGCCACTACCCATATATAGCACACAGTAATAATCAGATTCATCATCTG9850.11055627202005952No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG9840.11044403214998837No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC9760.10954611318941937No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT9630.10808699487849473No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9620.1079747550084236No Hit
ATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATTCTCAAACTGTGGTGACCCAGGAGCCATCGTT9440.10595443734714331No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC9430.1058421974770722No Hit
GGCCCAGGCAGATGATGAATCTGATTATTACTGTGTGCTATATATGGGTA9300.10438307916614757No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCGACAGCACCCCCCAAGATGGGAAC9220.10348516020557855No Hit
GTATCAACGCAGAGTACGGGAGCTGGGAAGCCTGAGAACACTTAGCCTTC9170.10292396085522292No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC9110.10225052163479616No Hit
GTCACACTCACTTGTGGCTTGACCTCTGGCTCAGTCTCTAGTAGTTACTA9080.10191380202458278No Hit
GTCTATTATAGTGGGAGCACCCACTACAACCCGTCCCTCAAGAGTCGAGT8990.10090364319394263No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC8990.10090364319394263No Hit
GGGCAGCTTTGTTCCCAAGGATGGAGCCAGAGAAGCGATCAGGGACCCCA8970.10067916345380039No Hit
CTAGTAGTTACTACCCCAGCTGGTACCAGCAGACCCCAGGCCAGGCTCCACGCACGCTCATCTACAGCACAAACA8920.10011796410344476No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)