FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691720

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691720
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences890949
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATGGGTAGTGGCATTGTGATCTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC45720.5131606859651899No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC37190.41742007679451915No Hit
GATTATTACTGTGTGCTATATATGGGTAGTGGCATTGTGATCTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT35400.39732914005178743No Hit
CCCATATATAGCACACAGTAATAATCAGATTCATCATCTGCCTGGGCCCCCGTGATGGTGAGGGCAGCTTTGTTC34710.38958458901687976No Hit
GTAATAATCAGATTCATCATCTGCCTGGGCCCCCGTGATGGTGAGGGCAG34340.38543171382424807No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGATCACAATGCCACTACCCATATATAGCACA33900.3804931595411185No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG31310.3514230331926968No Hit
CTCATCTACAGCACAAACACTCGCTCTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCCTTGGGAACAAAG29960.3362706507330947No Hit
GTAATACATAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGAGA28590.3208937885333504No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGATCACAATGCCACTACCCATATATAGCACACAGTAATAAT28280.3174143525611455No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT27760.3115778793174469No Hit
CACTTAGCCTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCT27070.3038333282825392No Hit
GTGCTATATATGGGTAGTGGCATTGTGATCTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG25500.2862116686813723No Hit
CTCTAGTAGTTACTACCCCAGCTGGTACCAGCAGACCCCAGGCCAGGCTCCACGCACGCTCATCTACAGCACAAA24210.271732725442197No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG23810.26724313063935196No Hit
GGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATTCTCAAACTGTGGTGACCCAGGAGCCAT23590.2647738534977872No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC22970.25781498155337734No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC22140.24849907233747384No Hit
ATGTATTACTGTGCGAGATGTTATAGGGGCTACGGTGACTACGTTGACTA21970.2465909945462647No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT21590.2423258794835619No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21090.2367138859800056No Hit
GTAGATGAGCGTGCGTGGAGCCTGGCCTGGGGTCTGCTGGTACCAGCTGG20430.22930605455531128No Hit
CTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCCTTGGGAACAAAGCTGCCCTCACCATCACGGGGGCCCAGG20260.2273979767641021No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC20120.22582661858310632No Hit
GTCTCTGGTGGCTCCATCAGCGATAGTAGTTACTACTGGGGCTGGATCCG19710.22122478391019013No Hit
GTCTCTAGTAGTTACTACCCCAGCTGGTACCAGCAGACCCCAGGCCAGGC19660.2206635845598345No Hit
CTACTAGAGACTGAGCCAGAGGTCAAGCCACAAGTGAGTGTGACCGTCCCTCCAGGGGACACTGAGAACGATGG19650.2205513446897634No Hit
ATATAGCACACAGTAATAATCAGATTCATCATCTGCCTGGGCCCCCGTGA19550.21942894598905213No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG19430.2180820675481986No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT19320.2168474289774162No Hit
CCCCAGGCCAGGCTCCACGCACGCTCATCTACAGCACAAACACTCGCTCT19300.216622949237274No Hit
GGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGG18070.20281744521852543No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC17300.1941749752230487No Hit
CTTGTGGCTTGACCTCTGGCTCAGTCTCTAGTAGTTACTACCCCAGCTGGTACCAGCAGACCCCAGGCCAGGCTC17050.19136897847127052No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16580.18609370457792757No Hit
CCCCAGAAGAGCGAGTGTTTGTGCTGTAGATGAGCGTGCGTGGAGCCTGG16410.18418562678671843No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC16370.18373666730643393No Hit
CCCCTGGAGGGACGGTCACACTCACTTGTGGCTTGACCTCTGGCTCAGTC16270.18261426860572266No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA16190.18171634964515365No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG16140.181155150294798No Hit
GCCTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACT15900.17846139341309097No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG15730.17655331562188184No Hit
GATCAGGAGTGGATTCTCAAACTGTGGTGACCCAGGAGCCATCGTTCTCAGTGTCCCCTGGAGGGACGGTCACA15560.17464523783067268No Hit
CATCTACAGCACAAACACTCGCTCTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCCTTGGGAACAAAGCTGC15270.17139028159861003No Hit
GTGGTGACCCAGGAGCCATCGTTCTCAGTGTCCCCTGGAGGGACGGTCACACTCACTTGTGGCTTGACCTCTGG15250.17116580185846778No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA15150.1700434031577565No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG14990.1682475652366185No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT14610.16398245017391566No Hit
CACTTGTGGCTTGACCTCTGGCTCAGTCTCTAGTAGTTACTACCCCAGCTGGTACCAGCAGACCCCAGGCCAGG14610.16398245017391566No Hit
CAGTAATACATAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCTTCAGGGA14250.15994181485135514No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC13870.1556766997886523No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT13620.15287070303687417No Hit
GGAGTGGATTCTCAAACTGTGGTGACCCAGGAGCCATCGTTCTCAGTGTC13600.15264622329673191No Hit
ACTAGAGACTGAGCCAGAGGTCAAGCCACAAGTGAGTGTGACCGTCCCTCCAGGGGACACTGAGAACGATGGCTC13530.15186054420623402No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT13450.15096262524566503No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT13300.14927902719459812No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCGATAGTAGTTACTACTGGG13240.14860558797417134No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA13130.14737094940338896No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12690.1424323951202594No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC12660.14209567551004604No Hit
CTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATT12650.1419834356399749No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA12540.14074879706919252No Hit
GTATTACTGTGCGAGATGTTATAGGGGCTACGGTGACTACGTTGACTACTGGGGCCAGGGAACCCTGGTCACCGT12460.1398508781086235No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGATCACAATGCCACTAC12220.13715712122691648No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC12180.13670816174663197No Hit
CAGTAATAATCAGATTCATCATCTGCCTGGGCCCCCGTGATGGTGAGGGC12090.13569800291599182No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC11940.13401440486492494No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11840.13289200616421365No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT11790.13233080681385803No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA11650.13075944863286226No Hit
GGCCTGGGGTCTGCTGGTACCAGCTGGGGTAGTAACTACTAGAGACTGAG11410.12806569175115523No Hit
GTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGG11270.12649433357015946No Hit
GTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATTCTCAAA11210.1258208943497327No Hit
CACCATCACGGGGGCCCAGGCAGATGATGAATCTGATTATTACTGTGTGCTATATATGGGTAGTGGCATTGTGAT11200.12570865447966156No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG11190.12559641460959045No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG11160.12525969499937706No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC11160.12525969499937706No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC11000.12346385707823905No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC10930.12267817798774117No Hit
ACCCATATATAGCACACAGTAATAATCAGATTCATCATCTGCCTGGGCCCCCGTGATGGTGAGGGCAGCTTTGTT10690.11998442110603413No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGATCACAATGCCA10500.11785186357468273No Hit
CTACAACCCGTCCCTCAAGAGTCGAGTCTCCATATCCGTGGACACGTCCAAGAACCAATTCTCCCTGAAGCTGAG10460.11740290409439821No Hit
GTGATGGTGAGGGCAGCTTTGTTCCCAAGGATGGAGCCAGAGAAGCGATC10260.11515810669297569No Hit
CTCCATATCCGTGGACACGTCCAAGAACCAATTCTCCCTGAAGCTGAGCT10250.11504586682290457No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC10210.11459690734262006No Hit
ACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATTCTCAAACTGTGGTGACC10160.11403570799226442No Hit
GTCGCTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC10160.11403570799226442No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT10100.11336226877183768No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT10090.11325002890176655No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC10050.11280106942148205No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA9980.11201539033098415No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9920.11134195111055739No Hit
GCCTGGGCCCCCGTGATGGTGAGGGCAGCTTTGTTCCCAAGGATGGAGCC9890.11100523150034403No Hit
ATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATTCTCAAACTGTGGTGACCCAGGAGCCATCGTT9820.11021955240984613No Hit
GTATCAACGCAGAGTACGGGAGCTGGGAAGCCTGAGAACACTTAGCCTTC9560.10730131578799684No Hit
CTATTATAGTGGGAGCACCCACTACAACCCGTCCCTCAAGAGTCGAGTCTCCATATCCGTGGACACGTCCAAGAA9550.10718907591792573No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC9440.10595443734714331No Hit
GTCTATTATAGTGGGAGCACCCACTACAACCCGTCCCTCAAGAGTCGAGT9440.10595443734714331No Hit
AGCCACAAGTGAGTGTGACCGTCCCTCCAGGGGACACTGAGAACGATGGCTCCTGGGTCACCACAGTTTGAGAAT9390.10539323799678768No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC9390.10539323799678768No Hit
TTATTACTGTGTGCTATATATGGGTAGTGGCATTGTGATCTTCGGCGGAG9370.10516875825664544No Hit
GTCACACTCACTTGTGGCTTGACCTCTGGCTCAGTCTCTAGTAGTTACTA9330.10471979877636094No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG9240.10370963994572079No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGAT9050.10157708241436939No Hit
GCTTGGTCCCTCCGCCGAAGATCACAATGCCACTACCCATATATAGCACACAGTAATAATCAGATTCATCATCTG9040.10146484254429826No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT204.740024E-4103.3081870
GTGGTAT1600.059.92111
CACGGAT6850.058.31507570
CGAAAGA200.00624580853.9402166
CGCGTCT200.006455418753.49196664
TGGTATC1800.053.2243272
CGATTCA200.00667634853.03880360
GGTATCA6250.041.0887571
TAAGTCA1351.6107151E-840.81310770
GTATCAA16200.039.5246771
GACAGTA702.3388566E-839.132151
ACAGGTA801.7662387E-938.5207061
CCACGGA7200.034.57214469
GTATAAG807.577728E-834.2406271
TATAAGA701.0383683E-634.215642
ATAGTAC501.9557582E-434.2098773
TATCAAC18550.033.9389652
GAGTTTA2300.032.7519071
ATCAACG19850.031.3662323
TCAACGC19950.031.209014
Produced by FastQC (version 0.11.5)