FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691721

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691721
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences888980
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCAC20990.23611329838691533No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAAT19040.2141780467502081No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT14880.167382843258566No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA14430.16232086211163355No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13910.15647146167517828No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC13660.15365924992688249No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATGGACTCTAGAATAGGATTGCG13010.14634749938131342No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12900.14511012621206326No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG12840.1444351953924723No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA11720.1318364867601071No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC11650.13104906747058429No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC11050.12429975927467435No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCT11020.12396229386487886No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT10720.1205876397669239No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACC10430.11732547413890075No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTC9780.11001372359333168No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG9630.1083263965443542No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTT9610.10810141960449055No Hit
GGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGGACGATGGGCATGAAACTGTGGTTTG9350.10517671938626291No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA9280.10438930009674008No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCA9180.10326441539742176No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC9130.1027019730477626No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC3950.051.631472
GTGGTAT4050.050.3679471
GGTATCA16350.048.0343971
CGTAATA306.7735434E-445.3439122
TCGTATG901.8189894E-1242.25729440
CTCGTAT901.8189894E-1242.17284439
GTATCAA47500.040.5117381
CGTATGC1003.6379788E-1034.64948341
ATCTCGT1102.910383E-1134.39115537
CGAGCCC1400.033.9907215
ATCAACG57750.033.0195583
CGTAGAG1350.032.8241228
TCAACGC58250.032.7361264
CAACGCA59250.032.2983555
AACGCAG60150.031.9281066
CGATTGT3400.031.8737370
CGCATCT652.56924E-531.37604715
TATCAAC62050.030.8408972
ACGCAGA64050.029.982327
CGCAGAG64250.029.8889888
Produced by FastQC (version 0.11.5)