FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004691723

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691723
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2215905
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC118740.5358532969599329No Hit
GCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCAC117130.528587642520776No Hit
CTCCTGCACTGGGAGCAGGTCAAACATCGGGGCAGGCTATGATGTACACTGGTACCAGCACCTTCCAGGAACAGC70880.31986930847667205No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT69070.31170108826867576No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG66310.2992456806586925No Hit
GTCATAGGACTGGCAGTAATAATCACCCTCATCCTCAGCCTGGAGCCCAG63920.28846001972106206No Hit
GATGTACACTGGTACCAGCACCTTCCAGGAACAGCCCCCAGACTCCTCAT62290.28110410870502117No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTC59450.26828767478750215No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC51470.2322753006108114No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT50990.2301091427655969No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC47780.21562296217572502No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG47560.21463013983000173No Hit
GTGCAGGAGATGGTGACCCTCTGCCCTGGGGCCCCAGACACTGAGGGCGG47220.2130957780229748No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT46540.21002705440892094No Hit
GTGATGGCCAGGGAGGCTGAGGTGGCAGACTTGGAGCCAGAGAAACGATT45360.2047019163727687No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT45010.20312242627729982No Hit
GGCTATGATGTACACTGGTACCAGCACCTTCCAGGAACAGCCCCCAGACTCCTCATCTATGGTAACATCAATCGG44730.20185883420092468No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT44200.19946703491350035No Hit
GTCCTATGACAGCAGCCTGACTGCTGATGTGGTGTTCGGCGGAGGGACCA44180.1993767783366164No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG43190.19490907778086153No Hit
GTGTACATCATAGCCTGCCCCGATGTTTGACCTGCTCCCAGTGCAGGAGA42700.19269779164720507No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG40440.1824987984593202No Hit
GGCCATCACTGGGCTCCAGGCTGAGGATGAGGGTGATTATTACTGCCAGT40370.18218290044022645No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC40310.18191213070957463No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC40100.1809644366522933No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG39370.17767007159602963No Hit
GTCTGGGGGCTGTTCCTGGAAGGTGCTGGTACCAGTGTACATCATAGCCT38780.17500750257795347No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA38740.17482698942418562No Hit
ACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCACATCAGCAGTCA36970.1668392823699572No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT36920.16661364092774736No Hit
GACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCACATCAGCAGTC36730.16575620344734995No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC36720.16571107515890798No Hit
CCCTCATCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGGC36350.16404132848655517No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG36320.16390594362122923No Hit
GCCCCCAGACTCCTCATCTATGGTAACATCAATCGGCCCTCAGGAGTCTCTAATCGTTTCTCTGGCTCCAAGT35970.16232645352576036No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA35660.16092747658405934No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA35540.16038593712275573No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG35520.16029568054587176No Hit
GGATGAGGGTGATTATTACTGCCAGTCCTATGACAGCAGCCTGACTGCTG34950.15772336810467957No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT33820.15262387151073714No Hit
GTTCCTGGAAGGTGCTGGTACCAGTGTACATCATAGCCTGCCCCGATGTT33600.15163104916501385No Hit
TCCAGGAACAGCCCCCAGACTCCTCATCTATGGTAACATCAATCGGCCCTCAGGAGTCTCTAATCGTTTCTCTGG33590.15158592087657188No Hit
GGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGGTCAAACATCGGGGCAGGCTATGATGTACACTGGTACCAG33070.14923924987758952No Hit
GTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTG33050.14914899330070558No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTC32380.1461253979750937No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCACAT32000.1444105230142989No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT31940.1441397532836471No Hit
ACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGC31940.1441397532836471No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC31930.14409462499520512No Hit
CCACATCAGCAGTCAGGCTGCTGTCATAGGACTGGCAGTAATAATCACCC31630.14274077634194607No Hit
GCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGGCAGACTTGGAGCC30890.1394012829972404No Hit
AGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGT30590.13804743434398134No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA30080.13574589163344095No Hit
CCTGAGGGCCGATTGATGTTACCATAGATGAGGAGTCTGGGGGCTGTTCC30040.1355653784796731No Hit
GCCCTCAGGAGTCTCTAATCGTTTCTCTGGCTCCAAGTCTGCCACCTCAG29150.1315489608083379No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA28990.13082690819326642No Hit
TCACTGGGCTCCAGGCTGAGGATGAGGGTGATTATTACTGCCAGTCCTAT28920.13051101017417263No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC28810.130014599001311No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT28240.12744228656011877No Hit
CTCTAATCGTTTCTCTGGCTCCAAGTCTGCCACCTCAGCCTCCCTGGCCA28220.12735202998323483No Hit
GTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAG27930.12604330961841775No Hit
GTGCTGGTACCAGTGTACATCATAGCCTGCCCCGATGTTTGACCTGCTCCCAGTGCAGGAGATGGTGACCCTCTG27810.12550177015711414No Hit
GATTATTACTGCCAGTCCTATGACAGCAGCCTGACTGCTGATGTGGTGTTCGGCGGAGGGACCAAGCTGACCGTC27590.12450894781139082No Hit
ATGATGTACACTGGTACCAGCACCTTCCAGGAACAGCCCCCAGACTCCTCATCTATGGTAACATCAATCGGCCCT27520.12419304979229705No Hit
GTAATAATCACCCTCATCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGG27000.1218463787933147No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC26750.1207181715822655No Hit
GCTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCT26060.11760431967976967No Hit
CATCAGCAGTCAGGCTGCTGTCATAGGACTGGCAGTAATAATCACCCTCATCCTCAGCCTGGAGCCCAGTGATGG26060.11760431967976967No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC25590.11548329012299714No Hit
GGTCAAACATCGGGGCAGGCTATGATGTACACTGGTACCAGCACCTTCCA25170.11358790200843448No Hit
GTGTATTACTGTGCGAGAGTCCGCGAAGGTCCGCGAAGGTGGATCGACCC25140.11345251714310857No Hit
CGATTAGAGACTCCTGAGGGCCGATTGATGTTACCATAGATGAGGAGTCT25080.11318174741245675No Hit
GTAATACACAGCCGTGTCTTCAATTCTCAGGCTATTCAACTCCATGTAGG25060.11309149083557284No Hit
GCTCCAGGCTGAGGATGAGGGTGATTATTACTGCCAGTCCTATGACAGCAGCCTGACTGCTGATGTGGTGTTCGG25030.11295610597024691No Hit
CTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGTCTGTG24660.11128635929789409No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG24510.11060943497126455No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTC24490.11051917839438063No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC24450.11033866524061274No Hit
GCTTGGTCCCTCCGCCGAACACCACATCAGCAGTCAGGCTGCTGTCATAGGACTGGCAGTAATAATCACCCTCAT24430.11024840866372881No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT24380.11002276722151898No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC24200.10921045802956353No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT24110.10880430343358583No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG23820.10749558306876875No Hit
CTGCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCAC23780.10731506991500087No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC23530.10618686270395165No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA23190.10465250089692474No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA23090.10420121801250504No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCCAC23090.10420121801250504No Hit
CCTCAGGAGTCTCTAATCGTTTCTCTGGCTCCAAGTCTGCCACCTCAGCCTCCCTGGCCATCACTGGGCTCCAGG22820.10298275422457191No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT22760.1027119844939201No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG22510.1015837772828709No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC22430.10122275097533513No Hit
CGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCAT22340.10081659637935741No Hit
GCCCAGTGATGGCCAGGGAGGCTGAGGTGGCAGACTTGGAGCCAGAGAAA22240.10036531349493774No Hit
CTCCTGAGGGCCGATTGATGTTACCATAGATGAGGAGTCTGGGGGCTGTTCCTGGAAGGTGCTGGTACCAGTGTA22230.10032018520649577No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG22220.1002750569180538No Hit
TCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGGCAGACTTGGAGCCAGAGAAACGATTAGAGACT22180.10009454376428593No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)