FastQCFastQC Report
Wed 8 May 2024
EGAF00004691724

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691724
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2215905
Total Bases160.6 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC126000.5686164343688019No Hit
GCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCAC116120.5240296853881371No Hit
CTCCTGCACTGGGAGCAGGTCAAACATCGGGGCAGGCTATGATGTACACT80850.3648622120533146No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG75110.33895857448762473No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG74340.3354836962775931No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT69910.3154918644978011No Hit
GTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCC68570.30944467384657737No Hit
GATGTACACTGGTACCAGCACCTTCCAGGAACAGCCCCCAGACTCCTCAT68280.30813595348176026No Hit
GTCATAGGACTGGCAGTAATAATCACCCTCATCCTCAGCCTGGAGCCCAG65620.2961318287561967No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC60200.27167229642064983No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA57740.26057073746392556No Hit
GTCCTATGACAGCAGCCTGACTGCTGATGTGGTGTTCGGCGGAGGGACCA55070.24852148444992No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA54570.24626507002782158No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG53780.24269993524090608No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCT53460.2412558300107631No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA52830.23841274783891908No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC52800.23827736297359317No Hit
GTGCAGGAGATGGTGACCCTCTGCCCTGGGGCCCCAGACACTGAGGGCGG52730.2379614649544994No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT50420.2275368303244047No Hit
GTGTACATCATAGCCTGCCCCGATGTTTGACCTGCTCCCAGTGCAGGAGA48060.21688655425210016No Hit
GGCTATGATGTACACTGGTACCAGCACCTTCCAGGAACAGCCCCCAGACT47640.2149911661375375No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT46460.2096660281013852No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA46040.20777063998682252No Hit
GTGATGGCCAGGGAGGCTGAGGTGGCAGACTTGGAGCCAGAGAAACGATT46020.20768038340993863No Hit
GGCCATCACTGGGCTCCAGGCTGAGGATGAGGGTGATTATTACTGCCAGT45250.20420550519990704No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTG44900.20262601510443812No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT44380.2002793441054558No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT43390.19581164354970093No Hit
GTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCAC42680.19260753507032116No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT42190.1903962489366647No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA41850.18886188712963778No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGT41810.1886813739758699No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA41530.1874177818994948No Hit
GGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGGTCAAACATCGGGGC41340.18656034441909738No Hit
GACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCACATCAGCAGTC40810.18416854513167305No Hit
GTCTGGGGGCTGTTCCTGGAAGGTGCTGGTACCAGTGTACATCATAGCCT40150.18119007809450316No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGC39760.17943007484526638No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC39390.17776032817291354No Hit
CTGCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGT39210.1769480189809581No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT38810.17514288744327938No Hit
GGATGAGGGTGATTATTACTGCCAGTCCTATGACAGCAGCCTGACTGCTG38180.17229980527143537No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC37960.17130698292571206No Hit
CCCTCATCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGGC37880.17094595661817633No Hit
GCCCCCAGACTCCTCATCTATGGTAACATCAATCGGCCCTCAGGAGTCTC37720.17022390400310483No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA37710.17017877571466286No Hit
ACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCACATCAGCAGTCA37490.16918595336893957No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT37330.16846390075386805No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA36630.16530492056293025No Hit
GCCCTCAGGAGTCTCTAATCGTTTCTCTGGCTCCAAGTCTGCCACCTCAG36390.16422184164032302No Hit
TCCAGGAACAGCCCCCAGACTCCTCATCTATGGTAACATCAATCGGCCCT36200.16336440415992562No Hit
GCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCT35710.16115311802626917No Hit
ACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCC35480.1601151673921039No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT34950.15772336810467957No Hit
GTTCCTGGAAGGTGCTGGTACCAGTGTACATCATAGCCTGCCCCGATGTT34210.15438387475997392No Hit
GGTCAAACATCGGGGCAGGCTATGATGTACACTGGTACCAGCACCTTCCA34070.15375207872178634No Hit
GTGCTGGTACCAGTGTACATCATAGCCTGCCCCGATGTTTGACCTGCTCC33980.15334592412580864No Hit
AGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTC33960.1532556675489247No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC33830.1526689997991791No Hit
CTCTAATCGTTTCTCTGGCTCCAAGTCTGCCACCTCAGCCTCCCTGGCCA33650.15185669060722368No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA32090.1448166776102766No Hit
GCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGGCAGACTTGGAGCC31560.1424248783228523No Hit
TCACTGGGCTCCAGGCTGAGGATGAGGGTGATTATTACTGCCAGTCCTAT31550.14237975003441034No Hit
ATGATGTACACTGGTACCAGCACCTTCCAGGAACAGCCCCCAGACTCCTC31440.1418833388615487No Hit
CTCCTCATCTATGGTAACATCAATCGGCCCTCAGGAGTCTCTAATCGTTT30810.13904025668970466No Hit
GTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAG30270.13660332911383835No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGA30170.13615204622941868No Hit
GCTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGG30150.13606178965253474No Hit
CCTGAGGGCCGATTGATGTTACCATAGATGAGGAGTCTGGGGGCTGTTCC29850.1347079409992757No Hit
CTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCA29790.1344371712686239No Hit
GTGTATTACTGTGCGAGAGTCCGCGAAGGTCCGCGAAGGTGGATCGACCC29520.13321870748069073No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTC29470.13299306603848088No Hit
CCACATCAGCAGTCAGGCTGCTGTCATAGGACTGGCAGTAATAATCACCC28980.13078177990482445No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGC28960.13069152332794048No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC28440.12834485232895815No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTG28400.12816433917519027No Hit
GTAATACACAGCCGTGTCTTCAATTCTCAGGCTATTCAACTCCATGTAGG28280.12762279971388665No Hit
GATTATTACTGCCAGTCCTATGACAGCAGCCTGACTGCTGATGTGGTGTT28240.12744228656011877No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCA28000.12635920763751154No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT27900.12590792475309184No Hit
GCTTGGTCCCTCCGCCGAACACCACATCAGCAGTCAGGCTGCTGTCATAG27650.12477971754204263No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG27520.12419304979229705No Hit
GGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGC27500.12410279321541312No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC27380.1235612537541095No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA27330.12333561231189964No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT26710.1205376584284976No Hit
GCTCCAGGCTGAGGATGAGGGTGATTATTACTGCCAGTCCTATGACAGCA26640.12022176040940383No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTC26510.11963509265965823No Hit
GTAATAATCACCCTCATCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGG26440.11931919464056448No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA25570.11539303354611323No Hit
CATCAGCAGTCAGGCTGCTGTCATAGGACTGGCAGTAATAATCACCCTCA25440.11480636579636763No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACA25260.11399405660441218No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGC25030.11295610597024691No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGG24970.1126853362395951No Hit
CCTCAGGAGTCTCTAATCGTTTCTCTGGCTCCAAGTCTGCCACCTCAGCC24690.11142174416322No Hit
CGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCAT24640.11119610272101015No Hit
CGATTAGAGACTCCTGAGGGCCGATTGATGTTACCATAGATGAGGAGTCT23970.10817250739539827No Hit
ATGGTAACATCAATCGGCCCTCAGGAGTCTCTAATCGTTTCTCTGGCTCC23860.10767609622253661No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT23830.1075407113572107No Hit
CTCCTGAGGGCCGATTGATGTTACCATAGATGAGGAGTCTGGGGGCTGTT23690.10690891531902316No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC23510.10609660612706771No Hit
CTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCG23280.10505865549290244No Hit
GCCCAGGACCCTGTGCAGTGAGCGAGGAGAGTGAGGAGGAGAGGAGACCA23200.1046976291853667No Hit
GGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGC23130.10438173116627293No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC22960.10361455026275945No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA22950.10356942197431748No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC22930.10347916539743356No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAA22750.10266685620547814No Hit
GTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGG22590.10194480359040663No Hit
GTACCAGCACCTTCCAGGAACAGCCCCCAGACTCCTCATCTATGGTAACA22580.10189967530196466No Hit
ATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTG22570.1018545470135227No Hit
GGGTGATTATTACTGCCAGTCCTATGACAGCAGCCTGACTGCTGATGTGG22400.10108736611000922No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)