FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691725

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691725
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1515336
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC72260.4768579377774962No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT53460.3527930439189724No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC47630.3143197284298664No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACAGTGCTGATGCTTGTATAAGAGCTG47280.31201000966122366No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACAGTGCTGATGCTTGTATAAGAGCTGCAGTAATAAT45800.30224319886810586No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG44400.2930043237935349No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG43230.28528326390978637No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT42600.28112577012622947No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG42560.280861802266956No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTT37900.2501095466615985No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG36410.24027674390366227No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT36370.2400127760443888No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC36370.2400127760443888No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC35320.23308361973846062No Hit
GAATACCACAGTGCTGATGCTTGTATAAGAGCTGCAGTAATAATCAGCTT34890.23024596525127097No Hit
GTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCG34570.22813422237708333No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC33810.2231188330508877No Hit
CATCAGCACTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA33520.22120506607115514No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT33490.22100709017670006No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG32740.21605769281532278No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC32560.21486983744859223No Hit
GTGCTGATGCTTGTATAAGAGCTGCAGTAATAATCAGCTTCGTCCTCAGC32350.2134840061874066No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC31510.20794068114266406No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG31490.20780869721302733No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTTTGTCTCCTGGTACCAACAGCACCCAGGCAAAGC31420.2073467534592988No Hit
CAGTAATACAGAGCCGTGTCCGCGGCGGTCACAGAGCTCAGCTCTAGGGA31230.20609290612774986No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT30840.20351921949983368No Hit
GATTATTACTGCAGCTCTTATACAAGCATCAGCACTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT30810.2033212436053786No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG30000.19797589445509112No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTTTGTCTCCTGGTACCAACAGCA29530.19487427210862807No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG29460.19441232835489952No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA28540.18834106759161004No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG27870.18391960594877968No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC27710.18286373451168586No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA27610.18220381486350223No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC26300.17355886747229657No Hit
GTAATAATCAGCTTCGTCCTCAGCCTGGAGCCCAGAGATTGTCAGGGAGG25770.1700612933369233No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG24990.16491392008109093No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC24860.1640560245384522No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA24470.161482337910536No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT24310.1604264664734422No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT24190.15963456289562183No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC23650.15607099679543018No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA23100.15244143873042018No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC22740.1500657279969591No Hit
GTTTGGGGGCTTTGCCTGGGTGCTGTTGGTACCAGGAGACAAAGTTATAA22610.14920783245432037No Hit
CCACAGTGCTGATGCTTGTATAAGAGCTGCAGTAATAATCAGCTTCGTCC21950.14485236277630836No Hit
GGTTATAACTTTGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATCATTTTTGATGTCAGTGAT21080.13911106183711072No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT20960.13831915825929036No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACAGTGCTGATGC20910.13798919843519855No Hit
GCACTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC20600.13594344752582926No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA20350.13429364840537017No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG20090.13257785732009272No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC19860.13106004212927036No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT19610.12941024300881127No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT19560.12908028318471945No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC19430.12822238764208072No Hit
GTATTACTGTGCGCGTCAACCCTACTACTATCGTTCGGGGAGTTATTATAATCGGTATTACTTTGACTACTGGGG19230.12690254834571343No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG19120.12617663673271143No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG18920.12485679743634415No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC18900.12472481350670742No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC18750.12373493403443196No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT18690.12333898224552177No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCCAC18620.12287703849179323No Hit
GCCCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAGTCTGGCAACACGG18430.12162319116024432No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC18330.12096327151206068No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG17820.11759768130632413No Hit
GGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATCATTTTTGATGTC17580.1160138741506834No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG17200.11350617948758558No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC17200.11350617948758558No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC16860.11126245268376121No Hit
ACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT16790.11080050893003267No Hit
GTTATAACCACCAACGTCACTGCTGGTTCCAGTGCAGGAGATGGTGATCG16690.11014058928184903No Hit
CGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCAT16660.10994261338739394No Hit
GCCTGGAGCCCAGAGATTGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC16560.1092826937392103No Hit
GTAATACAGAGCCGTGTCCGCGGCGGTCACAGAGCTCAGCTCTAGGGAGA16520.10901872587993686No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC16480.1087547580206634No Hit
CCCCTGAGGGCCGATCACTGACATCAAAAATGATGAGTTTGGGGGCTTTGCCTGGGTGCTGTTGGTACCAGGAGA16470.10868876605584503No Hit
GTCCAGGAGACCCAGACACGGAGGCAGGCTGAGTCAGGGCAGACTGGGCC16390.10816083033729812No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA16090.10618107139274721No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT16060.10598309549829212No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA16050.10591710353347375No Hit
GAATATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC15970.10538916781492684No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG15940.10519119192047176No Hit
GTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATCATTTTTGATGTCA15890.10486123209637993No Hit
CCTGAGGGCCGATCACTGACATCAAAAATGATGAGTTTGGGGGCTTTGCC15880.10479524013156158No Hit
GTCTTATAGTGGGAGCACCTACGACAACCCGTCCCTCAAGAGTCGAGTCACCACATCCGTAGACACGTCGAAGAA15630.10314544101110248No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC15590.10288147315182902No Hit
CAACAGCACCCAGGCAAAGCCCCCAAACTCATCATTTTTGATGTCAGTGATCGGCCCTCAGGGGTTTCTAATCG15540.10255151332773721No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA15200.10030778652391284No Hit
GTGCAGGAGATGGTGATCGACTGTCCAGGAGACCCAGACACGGAGGCAGG15170.10010981062945774No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG3250.047.6466140
GGTATCA10750.047.581641
CGTATGC3150.047.09517341
TCTACGG10350.046.2566770
TATCTCG3000.045.50557336
TCTCGTA3200.043.880838
ATCTCGT3250.043.1202437
AGGGGGG4400.042.57716470
ACGAGAC3800.041.6078422
CGTGCAA701.46828825E-841.25619557
ATGCCGT3750.040.76830744
GTATCAA32750.040.5872881
TGCCGTC3700.040.48502745
GCCTATC3450.039.3474433
TATACAC4350.038.663283
GCCGTCT3900.038.49833346
CTATCTC3650.038.26898635
GACCGGA4050.038.25048426
ACCGGAG4100.037.801527
GGATGGG8450.037.7717270
Produced by FastQC (version 0.11.5)