FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004691726

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691726
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1515336
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC68770.4538267420558873No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT49620.32745212942872076No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG48120.3175533347059662No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACAGTGCTGATGCTTGTATAAGAGCTG44960.29669987382336327No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC44910.2963699139992715No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG43980.29023266127116365No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG43550.287395006783974No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACAGTGCTGATGCTTGTATAAGAGCTGCAGTAATAAT42470.2802678745835907No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG38800.25604882349525127No Hit
GTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCG38770.2558508476007961No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC38740.25565287170634104No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG38020.25090145023941884No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT37130.24502816537058444No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTT35440.23387552331628098No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG34960.23070790900499955No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT34310.2264184312918059No Hit
GTGCTGATGCTTGTATAAGAGCTGCAGTAATAATCAGCTTCGTCCTCAGC33900.22371276073425297No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG33430.2206111383877899No Hit
GAATACCACAGTGCTGATGCTTGTATAAGAGCTGCAGTAATAATCAGCTT31830.2100524240168517No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC31630.20873258472048442No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC31630.20873258472048442No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTTTGTCTCCTGGTACCAACAGCACCCAGGCAAAGC31410.2072807614944804No Hit
CATCAGCACTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA31130.20543298647956623No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC31090.20516901862029277No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT30590.20186942037937458No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG30200.1992957337514584No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC30040.19823986231436458No Hit
GATTATTACTGCAGCTCTTATACAAGCATCAGCACTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT29590.19527022389753823No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC28650.18906697920461205No Hit
CAGTAATACAGAGCCGTGTCCGCGGCGGTCACAGAGCTCAGCTCTAGGGA28420.18754916401378968No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC28260.18649329257669586No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA27900.18411758184323476No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA27270.17996008805967786No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC27160.17923417644667586No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT27130.17903620055222078No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTTTGTCTCCTGGTACCAACAGCA26960.17791433715030858No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG26910.17758437732621676No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG24980.16484792811627258No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA24740.16326412096063184No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT24540.16194428166426456No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT24450.16135035398089928No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC23660.15613698876024856No Hit
GTAATAATCAGCTTCGTCCTCAGCCTGGAGCCCAGAGATTGTCAGGGAGG23470.15488314142869966No Hit
GTTTGGGGGCTTTGCCTGGGTGCTGTTGGTACCAGGAGACAAAGTTATAA22810.15052767175068765No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC22450.14815196101722655No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA21970.1449843467059451No Hit
GGTTATAACTTTGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATCATTTTTGATGTCAGTGAT21590.14247665204284726No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG21560.14227867614839218No Hit
GCCCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAGTCTGGCAACACGG20050.13231388946081926No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC20020.13211591356636418No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG19800.13066409034036017No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT19770.13046611444590508No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT19460.1284203635365358No Hit
CCACAGTGCTGATGCTTGTATAAGAGCTGCAGTAATAATCAGCTTCGTCC19330.12756246799389706No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT19230.12690254834571343No Hit
GCACTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC18820.1241968777881605No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC18740.1236689420696136No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACAGTGCTGATGC18470.12188715901951777No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA18340.12102926347687905No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC18300.12076529561760559No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC18140.11970942418051177No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC18090.11937946435641995No Hit
GTATTACTGTGCGCGTCAACCCTACTACTATCGTTCGGGGAGTTATTATAATCGGTATTACTTTGACTACTGGGG18000.11878553667305468No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG17840.11772966523596086No Hit
GGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATCATTTTTGATGTC17410.11489201074877121No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA17390.1147600268191345No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG17380.11469403485431613No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC17100.11284625983940194No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAAC17050.11251630001531013No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC16930.11172439643748976No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCCAC16870.11132844464857958No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT16800.11086650089485103No Hit
ACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT16760.11060253303557757No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG16740.11047054910594085No Hit
CCCCTGAGGGCCGATCACTGACATCAAAAATGATGAGTTTGGGGGCTTTGCCTGGGTGCTGTTGGTACCAGGAGA16260.1073029347946594No Hit
GTTATAACCACCAACGTCACTGCTGGTTCCAGTGCAGGAGATGGTGATCG15870.10472924816674321No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCG15840.10453127227228812No Hit
GCCTGGAGCCCAGAGATTGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC15840.10453127227228812No Hit
GTAATACAGAGCCGTGTCCGCGGCGGTCACAGAGCTCAGCTCTAGGGAGA15800.10426730441301466No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG15700.10360738476483103No Hit
GTCTTATAGTGGGAGCACCTACGACAACCCGTCCCTCAAGAGTCGAGTCACCACATCCGTAGACACGTCGAAGAA15660.10334341690555757No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA15230.10050576241836794No Hit
CGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCAT15190.10024179455909447No Hit
GTGCAGGAGATGGTGATCGACTGTCCAGGAGACCCAGACACGGAGGCAGG15160.10004381866463938No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)