FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691727

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691727
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1896232
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGATAGCCGTGTCGTCGACTCTCAGGCTGTTCAATTCCAGATAAA78120.4119749060241574No Hit
ATCTATTACTGCGCGAGAGATCCCCCGGGGGTGGGAACTTCTGACTTCTGGGGCCAGGGAACCCTGGTCACCGT62140.32770251741348105No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT53120.2801344983103333No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT49000.2584071991190951No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC47570.2508659277978644No Hit
ACCAAATACTACGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGA46660.24606693695708118No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCG43600.22992967105290915No Hit
GTATTTGGTAGCGCCACCAGCATTAATGTATGAAAGCCACTCCAGCCCCT40860.21547996236747402No Hit
CTCCTGTAGGGCCAGTCAGAGTGTTCGCGGAGGTTATTTAGCCTGGTTCC37950.2001337389095849No Hit
TCTATGGAGTATCCCACAGGCCCACTGGCGTCCCAGACAGGTTCAGTGCCAGAGGGTCTGGGACAGACTTCACT37250.19644220749359784No Hit
GTCTGGGACAGACTTCACTCTCTCCATCAGCAGACTGGAGCCTGAAGATT35700.18826810221534074No Hit
GTCTGGGGGTGGCTTGGTGCAGCCTGGAGGGTCCCACAGACTCTCCTGTG33340.17582236772715576No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC33230.17524226993321493No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32720.17255272561585291No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC32630.17207810014808314No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA31930.16838656873209606No Hit
GTCCCAGACCCTCTGGCACTGAACCTGTCTGGGACGCCAGTGGGCCTGTG31670.16701542849187231No Hit
GTTTTACTGTCAGCAGTATGCTACGCCGCCATTCACTTTCGGCGGAGGGA31340.16527513511004982No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA28310.14929607769513437No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT28180.14861050757502245No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27590.14549907395297623No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG27330.14412793371275245No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG27150.1431786827772129No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC26960.14217669567858784No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26790.14128018090613384No Hit
CTCCTGTGCAGCCTCTGGCTTGACCTTCAGTGAATATGATGTAAATTGGGTCCGCCAGGCTCCTGGGAAGGGG26220.13827421961025865No Hit
CCCCCAGACTGCGTCAGCTTCACCTCACATTGGACACCTTCTAAAATAGC26080.13753591332706125No Hit
CTCCATAGATGAGGAGCCTGGGAGCCTGGCCACTCTTCTTCTGGAACCAGGCTAAATAACCTCCGCGAACACT25920.13669213471769276No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG25750.13579561994523878No Hit
GCACTGAACACAGAGGACTCACCATGGACCTGGGGCTGTGCTGGGTTTTG25620.1351100498251269No Hit
GTGCCAGAGGGTCTGGGACAGACTTCACTCTCTCCATCAGCAGACTGGAGCCTGAAGATTTTGCAATGTTTTA25600.13500457749895584No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC25510.13452995203118606No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG25460.1342662712157584No Hit
GACTTCACTCTCTCCATCAGCAGACTGGAGCCTGAAGATTTTGCAATGTTTTACTGTCAGCAGTATGCTACGCCG25420.1340553265634163No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG25180.1327896586493636No Hit
GCCTGAGAGTCGACGACACGGCTATCTATTACTGCGCGAGAGATCCCCCG24890.13126030991988322No Hit
GTGCAGCCACAGTTCGCTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG24710.13031105898434367No Hit
GTGCTGGGTTTTGCTTGTTGCTATTTTAGAAGGTGTCCAATGTGAGGTGA24670.13010011433200158No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24430.12883444641794886No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA24230.12777972315623826No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC24080.1269886807099553No Hit
CTCCAGGCACCCTGGCTTTGTCGCCAGGGGACAGCGCCACCCTCTCCTGT23680.12487923418653414No Hit
CAGTAAAACATTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGAGAGAGTGAAGTCTGTCCCAGACCCTCTGGCA23550.12419366406642225No Hit
GAGTGAAGTCTGTCCCAGACCCTCTGGCACTGAACCTGTCTGGGACGCCA23360.1231916769677972No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23000.12129317509671812No Hit
CTCCATCAGCAGACTGGAGCCTGAAGATTTTGCAATGTTTTACTGTCAGCAGTATGCTACGCCGCCATTCACTTT22910.12081854962894835No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22790.120185715671922No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22450.118392686127014No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC21880.11538672483113879No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC21720.11454294622177033No Hit
GTATGCTACGCCGCCATTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAGCGAACTGTGGCTGCACCAT21600.11391011226474397No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21170.11164245725206619No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21160.11158972108898067No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATCTCCACCTTGGTCC21130.11143151259972409No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT20740.10937480223938843No Hit
CATCTATGGAGTATCCCACAGGCCCACTGGCGTCCCAGACAGGTTCAGTGCCAGAGGGTCTGGGACAGACTTCA20710.10921659375013183No Hit
CTCTCAGGCTGTTCAATTCCAGATAAACCGAGTTCTTGGCGTCGTCTCTG20640.10884744060853313No Hit
CATTAATGCTGGTGGCGCTACCAAATACTACGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACGACGC20450.10784545350990807No Hit
CCATGGACCTGGGGCTGTGCTGGGTTTTGCTTGTTGCTATTTTAGAAGGT20330.10721261955288172No Hit
GTCAGAGTGTTCGCGGAGGTTATTTAGCCTGGTTCCAGAAGAAGAGTGGC19960.10526138151871713No Hit
CTCCAGAGACGACGCCAAGAACTCGGTTTATCTGGAATTGAACAGCCTGAGAGTCGACGACACGGCTATCTATTA19540.10304646266912489No Hit
GCATTAATGTATGAAAGCCACTCCAGCCCCTTCCCAGGAGCCTGGCGGAC19440.10251910103826957No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT19430.10246636487518405No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG19320.10188626708124322No Hit
CAGTAATAGATAGCCGTGTCGTCGACTCTCAGGCTGTTCAATTCCAGATA19310.10183353091815771No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC19130.10088427998261816No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG8300.050.57740440
CGTATGC8500.049.9394141
ACATGCG8350.048.3209727
GCCGTCT8850.048.202246
ATGCCGT8800.048.19520644
CCGTCTT9100.047.0240447
GATCTCG8100.046.6723336
CATGCGC8750.046.5356428
ATCTCGT8150.046.496837
ATGCGCA8800.046.3263929
GTATGCC9300.046.14986442
CTTATAC8950.046.1257061
TATACAC9300.045.4460873
CACGAGA9200.044.4886921
CGCAGAT9050.044.10620532
TCTCCGA9300.043.9998711
ATCTCCG9300.043.9987110
ACGAGAC9350.043.7784322
CCCACGA9450.042.95219419
CGAGACA9550.042.8683923
Produced by FastQC (version 0.11.5)