FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691728

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691728
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1896232
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG86690.4571697977884563No Hit
GTAATAGATAGCCGTGTCGTCGACTCTCAGGCTGTTCAATTCCAGATAAA69700.36757105670614143No Hit
ATCTATTACTGCGCGAGAGATCCCCCGGGGGTGGGAACTTCTGACTTCTGGGGCCAGGGAACCCTGGTCACCGT59240.3124090301186775No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT53240.28076733226735967No Hit
ACCAAATACTACGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGA42700.22518341637521147No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC41350.21806403435866498No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCG41310.21785308970632286No Hit
GTATTTGGTAGCGCCACCAGCATTAATGTATGAAAGCCACTCCAGCCCCT39100.2061983976644208No Hit
CTCCTGTAGGGCCAGTCAGAGTGTTCGCGGAGGTTATTTAGCCTGGTTCC38180.2013466706605521No Hit
GTCTGGGACAGACTTCACTCTCTCCATCAGCAGACTGGAGCCTGAAGATT36770.19391087166549242No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC34140.18004126077399812No Hit
GTCTGGGGGTGGCTTGGTGCAGCCTGGAGGGTCCCACAGACTCTCCTGTG33290.1755586869117281No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC32780.1728691425943661No Hit
TCTATGGAGTATCCCACAGGCCCACTGGCGTCCCAGACAGGTTCAGTGCCAGAGGGTCTGGGACAGACTTCACT32240.1700213897877475No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC30850.16269106311885886No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30480.16073982508469428No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30220.1593686848444705No Hit
GTTTTACTGTCAGCAGTATGCTACGCCGCCATTCACTTTCGGCGGAGGGA30060.15852490623510204No Hit
GTCCCAGACCCTCTGGCACTGAACCTGTCTGGGACGCCAGTGGGCCTGTG29730.15678461285327958No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA27820.1467120057039434No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT27510.14507718464829197No Hit
GTGCTGGGTTTTGCTTGTTGCTATTTTAGAAGGTGTCCAATGTGAGGTGA27500.14502444848520646No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27270.14381151673423928No Hit
CTCCTGTGCAGCCTCTGGCTTGACCTTCAGTGAATATGATGTAAATTGGGTCCGCCAGGCTCCTGGGAAGGGG26790.14128018090613384No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA26770.14117470857996278No Hit
GTGCCAGAGGGTCTGGGACAGACTTCACTCTCTCCATCAGCAGACTGGAGCCTGAAGATTTTGCAATGTTTTA25540.13468816052044263No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC25380.13384438191107417No Hit
GTGCAGCCACAGTTCGCTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG25270.13326428411713334No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG25250.13315881179096228No Hit
GACTTCACTCTCTCCATCAGCAGACTGGAGCCTGAAGATTTTGCAATGTTTTACTGTCAGCAGTATGCTACGCCG25120.1324732416708504No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT24430.12883444641794886No Hit
GCACTGAACACAGAGGACTCACCATGGACCTGGGGCTGTGCTGGGTTTTG24320.12825434862400803No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA24290.12809614013475143No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24240.1278324593193238No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG24030.12672499989452768No Hit
GAGTGAAGTCTGTCCCAGACCCTCTGGCACTGAACCTGTCTGGGACGCCA23970.12640858291601448No Hit
CTCCATAGATGAGGAGCCTGGGAGCCTGGCCACTCTTCTTCTGGAACCAGGCTAAATAACCTCCGCGAACACT23920.12614490210058685No Hit
CCCCCAGACTGCGTCAGCTTCACCTCACATTGGACACCTTCTAAAATAGC23840.12572301279590262No Hit
GCCTGAGAGTCGACGACACGGCTATCTATTACTGCGCGAGAGATCCCCCG23700.12498470651270518No Hit
GTCTCCAGGCACCCTGGCTTTGTCGCCAGGGGACAGCGCCACCCTCTCCT23610.12451008104493544No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23140.12203148137991554No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22760.12002750718266542No Hit
CTCCAGGCACCCTGGCTTTGTCGCCAGGGGACAGCGCCACCCTCTCCTGT22670.11955288171489566No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC22410.11818174147467186No Hit
CAGTAAAACATTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGAGAGAGTGAAGTCTGTCCCAGACCCTCTGGCA22270.11744343519147446No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22210.11712701821296129No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22200.11707428204987574No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22120.11665239274519153No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21920.11559766948348092No Hit
CTCCATCAGCAGACTGGAGCCTGAAGATTTTGCAATGTTTTACTGTCAGCAGTATGCTACGCCGCCATTCACTTT21890.11543946099422434No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT21610.11396284842782951No Hit
GTATGCTACGCCGCCATTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAGCGAACTGTGGCTGCACCAT20760.10948027456555949No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC20450.10784545350990807No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT19930.10510317302946054No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATCTCCACCTTGGTCC19620.10346835197380912No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC19530.10299372650603934No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19460.10262457336444064No Hit
GTCAGAGTGTTCGCGGAGGTTATTTAGCCTGGTTCCAGAAGAAGAGTGGC19410.102360892549013No Hit
CTCCAGAGACGACGCCAAGAACTCGGTTTATCTGGAATTGAACAGCCTGAGAGTCGACGACACGGCTATCTATTA19250.10151711393964452No Hit
CTCTCAGGCTGTTCAATTCCAGATAAACCGAGTTCTTGGCGTCGTCTCTG19240.10146437777655899No Hit
CATTAATGCTGGTGGCGCTACCAAATACTACGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACGACGC19220.10135890545038793No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG19010.10025144602559181No Hit
GCATTAATGTATGAAAGCCACTCCAGCCCCTTCCCAGGAGCCTGGCGGAC18970.10004050137324969No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15300.036.8260541
ATAGCGA400.002108236335.99713555
GTATCAA37900.035.7502821
GTGGTAT5550.033.8401571
TCTGGAG26550.033.49397370
TATCAAC41800.032.1570432
ATCAACG43000.031.1709883
TCAACGC43450.030.9260944
CAACGCA44000.030.5411325
AACGCAG44800.029.993386
AATGGTA11200.029.15419470
TCACCGT20250.028.97206768
TAGCGAG500.006192734528.88695356
TCGTCTC20200.028.62970565
AGAGTAC46700.028.55481711
CGCAGAG47550.028.1875328
CGTCGTC20900.028.09875163
TGGTATC6450.028.0635152
ACTAGTG500.007161942328.03954147
GTCGTCT20950.027.8111564
Produced by FastQC (version 0.11.5)