FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004691732

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691732
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1916763
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATAGTGTTCCCCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT135980.7094252132371086No Hit
CTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGTTGATGGTGAGAGTGAACTCTGTC128260.6691489766862153No Hit
CTTTTATACAGCTCCAATAGTAAGAACTACTTAGCTTGGTACCAGCAGAA101190.5279212923037433No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT89710.4680286503860936No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC71630.3737029564948823No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC66000.3443305197356168No Hit
GTATAAAAGACTCTGGCTGGACTTGCAGTTGAAGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA61150.3190274436641358No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC60780.31709710590198165No Hit
GAGTTCACTCTCACCATCAACAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAGTGTT54730.2855334749262168No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC52580.27431664738937467No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT52160.27212545317287534No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG51760.270038601538114No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGGGT48470.25287424684220217No Hit
ACTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTACTATTGGAGCTGTATAAAAGACT48260.25177864973395253No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA47360.24708323355573952No Hit
CTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT45740.23863148443495621No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT43290.22584951817204318No Hit
GAGTGAACTCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCG43130.22501477751813867No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG42860.22360615266467476No Hit
GTTTATTACTGTCAGCAATATTATAGTGTTCCCCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA42130.21979764843123537No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT41860.2183890235777715No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGTTGATGGTGAGAGTGAACTCTGTCCCAGACCCGCTGCCA41600.21703257001517662No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC41400.21598914419779597No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT40900.21338057965434432No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG39050.20372889084357323No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG39020.20357237697096617No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC38980.20336369180749No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT38680.20179855308141903No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG38660.20169421049968098No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG38650.20164203920881194No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG37780.19710313690320608No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG37030.19319029008802863No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG37020.19313811879715959No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA35940.18750361938330404No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT35360.18447768451290014No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCTTCAACTGCAAGTCCAGCCAGAGTCTTTTATA35330.18432117064029302No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGAGTTCACTCTCAC32680.17049577855999934No Hit
AACCAGGACAGCCTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGAA31960.16673944561742898No Hit
GCTCCAATAGTAAGAACTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGTTGCTCATTTACTGGG31810.16595687625439348No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAACAGCCTGCAGGCTGAAGATG31370.16366133945615605No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA30680.16006152038619276No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG29640.15463570613581334No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA29610.15447919226320625No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC29260.1526531970827901No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG28980.15119240093845718No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG28600.14920989188543393No Hit
GTAATATACGGCTGTGTCCTCGGTTTTCAGGCTGTTCATTTGTAGATACA28320.147749095741101No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27870.14540138765199453No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCTTCAACTGCAAGTCCA27850.14529704507025648No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA27190.14185373987290031No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA27050.14112334180073383No Hit
GTCAGGGACCCCGGATTCCCGGGTAGATGCCCAGTAAATGAGCAACTTAGGAGGCTGTCCTGGTTTCTGCTGGTA26690.13924517532944866No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT26580.1386712911298893No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT25410.13256725009821244No Hit
GTTGATGGTGAGAGTGAACTCTGTCCCAGACCCGCTGCCACTGAATCGGT25280.13188902331691504No Hit
TCTTCAGCCTGCAGGCTGTTGATGGTGAGAGTGAACTCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC25030.13058474104518922No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT24810.1294369726460705No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC24660.12865440328303498No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA24600.1283413755378208No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTACTATTGGAGCTG24540.1280283477926066No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC24320.12688057939348787No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT24290.12672406552088078No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA24220.12635886648479755No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCTTCAACTGCAAGTCCAGC23670.12348944548700073No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC23540.12281121870570333No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC23430.12223733450614396No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC23340.12176779288832265No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGAGTTCACTCTCACCATCAACAGCC23280.12145476514310845No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT23080.1204113393257278No Hit
CACCATCAACAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAGTGTTCCCCTCACTTT23060.12030699674398974No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT22760.11874185801791874No Hit
GTCTCTGGGCGAGAGGGCCACCTTCAACTGCAAGTCCAGCCAGAGTCTTTTATACAGCTCCAATAGTAAGAACTA22540.11759408961880002No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGTTGATGGTGAGAGTGAACTCTGTCCCAGACCCGCTGCCA22430.11702020541924066No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC22400.11686369154663358No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC22140.11550723798403871No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC21750.11347255764014644No Hit
ATTATAGTGTTCCCCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAA21570.11253347440450384No Hit
GGATCTCTGGTGCCTGCGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG21050.10982056727931414No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC21000.10955971082496896No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA20790.10846411371671928No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT20690.10794240080802896No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT20630.10762937306281475No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA20610.1075250304810767No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC20500.10695114628151733No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC20390.10637726208195797No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA20370.1062729195002199No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT20230.10554252142805343No Hit
GGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAGTGTTCCCC19800.10329915592068503No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA19730.1029339568846018No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT19670.1026209291393876No Hit
CTATTGGAGCTGTATAAAAGACTCTGGCTGGACTTGCAGTTGAAGGTGGC19500.10173401719461406No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG19490.101681845903745No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG19430.10136881815853083No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC19400.10121230428592372No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT19380.10110796170418564No Hit
ACCTTCAACTGCAAGTCCAGCCAGAGTCTTTTATACAGCTCCAATAGTAA19250.10042973492288823No Hit
CTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGAATC19220.10027322105028112No Hit
GAACTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGTTGC19190.10011670717767403No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)