FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691734

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691734
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences797146
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24630.3089772764336772No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT11870.14890622295037548No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC9360.11741889189684199No Hit
CTACAAACTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACG8900.11164830532926213No Hit
GAGTTTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAAT8810.11051927752256174No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC8530.1070067465683827No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9950.040.8304331
GTATCAA24950.037.9938471
GTGGTAT3300.037.9588971
TGGTATC3500.035.7673532
ATCAACG28950.032.367013
TATCAAC29000.032.3172872
TCAACGC29500.031.8802284
CAACGCA30300.031.375425
AACGCAG30900.030.766196
ACGCAGA33450.028.4207847
CGCAGAG34450.027.7939518
AGAGTAC35300.027.21541811
TATATCG500.00850768627.0654855
CAGAGTA36650.026.30688710
TCTGTCG1458.8039087E-1025.6671588
CGATTCA851.0681868E-425.45497360
GCAGAGT40100.024.2951439
GTGTAAT1401.6943886E-824.1823081
CTGTCGC1551.9135769E-924.0066939
GCGTAGA7050.022.77764370
Produced by FastQC (version 0.11.5)