FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004691735

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691735
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1889382
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC82970.43913830024844097No Hit
GTATTAGTACCTGGTTGGCCTGGTATCAACAGAAAGCAGGGAAAGCCCCT79600.42130178015880326No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT75900.4017186572117232No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC65520.3467800582412662No Hit
GTACTAATACTCTGGCTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCAGACAGGGTGGAAGGGGA61270.32428593053178234No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC59570.3152882794479888No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA54990.2910475488810627No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC54300.28739556108822883No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG49590.2624667748501891No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG49440.26167286446044263No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG48700.2577562398710266No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT47140.24949957181766313No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC46900.24822931519406874No Hit
GAGTATTAGTACCTGGTTGGCCTGGTATCAACAGAAAGCAGGGAAAGCCCCTAAGTTCCTGATCTATAAGGCGT45200.23923166411027522No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTCTGCAACTTATTACTGCCAGCAGTATTTTAATTAC45130.23886117259506018No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT44120.23351550930410048No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG43070.22795813657587508No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG43060.22790520921655866No Hit
CTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGG42410.22446493086099054No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT42240.22356516575261115No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG41040.21721388263463925No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT40530.21451458730950118No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC40400.2138265316383876No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT38720.20493473527322692No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG38480.20366447864963252No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGCCAGAGTATTAGTAC36760.1945609728472061No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG36130.19122654921027085No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT35760.18926823691556288No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT35530.18805090765128493No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGCCAGA35520.1879979802919685No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG34860.18450477457708395No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGCTGGGACCCCA34210.18106449622151582No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT33400.1767773801168848No Hit
CTAATACTCTGGCTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA31470.1665623997688133No Hit
CCTTATAGATCAGGAACTTAGGGGCTTTCCCTGCTTTCTGTTGATACCAGGCCAACCAGGTACTAATACTCTGG31360.16598019881633252No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG31230.16529214314521892No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG29910.15830573171544982No Hit
GTATTTTAATTACCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT28830.1525895769092751No Hit
ACTTATTACTGCCAGCAGTATTTTAATTACCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA28820.15253664954995866No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA28800.1524307948313258No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCCCCTTCCACC28320.14989028158413703No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28080.14862002496054266No Hit
GGGTGGAAGGGGACTGGGTCATCTGGATGTCACATTTGGCACCTGGGAGCCAGAGCAGCAGGAGCCCCAGGAG27690.14655585794720177No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG27300.1444916909338609No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC27300.1444916909338609No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA26680.1412101946562421No Hit
GGTATCAACAGAAAGCAGGGAAAGCCCCTAAGTTCCTGATCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCCCAG26530.1404162842664956No Hit
GTCCCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGCCAGAGTATTAGTAC26360.13951651915811625No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT25860.13687015119229462No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA25380.13432963794510586No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT25330.13406500114852368No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG24880.13168326997928423No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC24690.13067765015227203No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC24340.12882519257619687No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC24190.12803128218645038No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT24110.12760786331191892No Hit
GTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCC24060.12734322651533678No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT23690.12538491422062875No Hit
ACCCAGTCCCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCAT23590.12485564062746442No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC23370.12369123872250291No Hit
CTACTACAGTAATACACGGCTGTGTCCTCGGTTTTCAGGCTATTCATTTG23310.12337367456660432No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT23280.12321489248865501No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA23230.12295025569207287No Hit
CACCATCAGCAGCCTGCAGCCTGATGATTCTGCAACTTATTACTGCCAGCAGTATTTTAATTACCCGCTCACTTT23200.12279147361412356No Hit
GTAATAAGTTGCAGAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG23200.12279147361412356No Hit
GTCTCCTACAGATGCAGACAGGGTGGAAGGGGACTGGGTCATCTGGATGTCACATTTGGCACCTGGGAGCCAGAG22980.12162707170916204No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC22880.12109779811599773No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT22830.12083316131941556No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC22660.1199333962110362No Hit
TCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCCCAGCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT22580.11950997733650474No Hit
CTCCTGTACAGTTTCTGGATTCACTATTGGTGATTATGCTATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGG22170.11733995560453102No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT22160.11728702824521457No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA21870.11575213482503803No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT21740.1150640791539244No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA21680.11474651499802581No Hit
GTTGATACCAGGCCAACCAGGTACTAATACTCTGGCTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATG21230.11236478382878634No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC21150.11194136495425487No Hit
ACCTTGCTGGGACCCCACTTTCTAAACTAGACGCCTTATAGATCAGGAAC20900.11061818097134407No Hit
GCACTGAACACAGACCACCAACCATGGAGTTTGGGCTTAGCTGGGTTTTC20760.10987719794091401No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG20610.10908328755116753No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT20430.10813059508347174No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA20110.1064369195853459No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG19910.10537837239901725No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT19850.10506080824311864No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA19730.10442567993132144No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA19400.10267907707387919No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC19290.10209687612139844No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG19270.10199102140276556No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC18930.10019149118600686No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC18900.10003270910805755No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)