FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004691736

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691736
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1889382
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG84530.44739496830180453No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT73450.3887514541791972No Hit
GTATTAGTACCTGGTTGGCCTGGTATCAACAGAAAGCAGGGAAAGCCCCT73250.38769290699286857No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC71600.3789598927056572No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC64140.3394760826555985No Hit
GTACTAATACTCTGGCTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCAGACAGGGTGGAAGGGGA60140.31830513892902546No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC57550.3045969528660694No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC54760.28983021961678473No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA51210.27104100705945117No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC47580.25182837562758614No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT46780.24759418688227158No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG44850.23737920653420008No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG43790.2317689064466582No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT43160.22843448280972295No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC42890.22700544410817927No Hit
GAGTATTAGTACCTGGTTGGCCTGGTATCAACAGAAAGCAGGGAAAGCCCCTAAGTTCCTGATCTATAAGGCGT42670.22584104220321777No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT42420.22451785822030695No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGCTGGGACCCCA41990.22224198176970036No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTCTGCAACTTATTACTGCCAGCAGTATTTTAATTAC41430.21927804964798014No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG40530.21451458730950118No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG40500.2143558052315519No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG39440.20874550514401008No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG38050.20138860219902593No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT37410.19800125120277423No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT37070.19620172098601552No Hit
CTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGG36700.19424340869130752No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG35320.18693943310563985No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT34960.18503404817024827No Hit
GGGTGGAAGGGGACTGGGTCATCTGGATGTCACATTTGGCACCTGGGAGCCAGAGCAGCAGGAGCCCCAGGAG34670.1834991547500717No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGCCAGA34250.18127620565878155No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT34010.18000594903518716No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT33100.1751895593373918No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG32930.17428979422901247No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG32480.171908063059773No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGCCAGAGTATTAGTAC32360.17127293474797578No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA29940.1584645137933991No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG29510.1561886373427925No Hit
CTAATACTCTGGCTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGA29320.15518301751578029No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA28850.15269543162790797No Hit
CCTTATAGATCAGGAACTTAGGGGCTTTCCCTGCTTTCTGTTGATACCAGGCCAACCAGGTACTAATACTCTGG28580.1512663929263643No Hit
GTATTTTAATTACCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT28330.14994320894345348No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG28050.14846124288259335No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA27710.14666171266583464No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCCCCTTCCACC26470.140098720110597No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG25970.13745235214477539No Hit
ACTTATTACTGCCAGCAGTATTTTAATTACCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA25500.13496476625690304No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC25430.13459427474168803No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA24710.13078350487090487No Hit
GGTATCAACAGAAAGCAGGGAAAGCCCCTAAGTTCCTGATCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCCCAG24570.13004252184047482No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC24510.12972495768457623No Hit
GTCCCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGCCAGAGTATTAGTAC24310.12866641049824756No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT24230.1282429916237161No Hit
GTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCC23950.12676102556285598No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC23800.12596711517310952No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC23610.1249614953460973No Hit
GTCTCCTACAGATGCAGACAGGGTGGAAGGGGACTGGGTCATCTGGATGTCACATTTGGCACCTGGGAGCCAGAG23550.1246439311901987No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA23490.1243263670343001No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT23290.12326781984797144No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22790.12062145188214983No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG22780.1205685245228334No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC22610.11966875941445403No Hit
GTAATAAGTTGCAGAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG22590.11956290469582118No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT22230.1176575197604296No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC22170.11733995560453102No Hit
CTCCTGTACAGTTTCTGGATTCACTATTGGTGATTATGCTATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGG21810.11543457066913944No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA21670.11469358763870938No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC21230.11236478382878634No Hit
TCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCCCAGCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT21200.11220600175083704No Hit
CACCATCAGCAGCCTGCAGCCTGATGATTCTGCAACTTATTACTGCCAGCAGTATTTTAATTACCCGCTCACTTT21180.1121001470322042No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT21070.11151794607972342No Hit
GTTGATACCAGGCCAACCAGGTACTAATACTCTGGCTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATG21050.11141209136109056No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT20890.11056525361202764No Hit
CTACTACAGTAATACACGGCTGTGTCCTCGGTTTTCAGGCTATTCATTTG20560.10881865075458536No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC20390.10791888564620601No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA20290.10738961205304168No Hit
ACCTTGCTGGGACCCCACTTTCTAAACTAGACGCCTTATAGATCAGGAAC20200.1069132658191938No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGCGGGTAATT20010.10590764599218157No Hit
GTCCCAGATCCACTGCCGCTGAACCTTGCTGGGACCCCACTTTCTAAACT19870.10516666296175152No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC19790.10474324408722005No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT19630.10389640633815714No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT19620.10384347897884069No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC19600.10373762426020784No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT19430.10283785915182848No Hit
GCACTGAACACAGACCACCAACCATGGAGTTTGGGCTTAGCTGGGTTTTC19100.1010912562943862No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)