FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004691737

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691737
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences930863
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCATTATGGTAGCTCACCGCGCACTTTCGGCGGAGGGA65940.7083749166096407No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT52320.5620590785110161No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGGCA47410.5093123263036559No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC41610.44700455383874965No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA38100.40929760877809085No Hit
CATCTATACTACATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGCCAGTGGGTCTGGGACAGACTTCA36020.38695275244584865No Hit
GTGTATTACTGTCAGCATTATGGTAGCTCACCGCGCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA33200.3566582837646356No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC31610.3395773599337389No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG31260.3358174081470635No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28750.30885318247690585No Hit
ATACTACATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGCCAGTGGGTCTGGGACAGACTTCACTCTCA27930.3000441525766949No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT27800.29864759905592986No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27080.29091284109476906No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGGCA26580.2855414813995185No Hit
CTCCTGCAGGGCCAGTCAGAGTCTTAGAAGCAGCTACTTAGCCTGGTATC25840.2775918690505477No Hit
GTATAGATGAGGAGCCTGGGAGCCTGGACAGGCTTCTGCTGATACCAGGCTAAGTAGCTGCTTCTAAGACTCTGA25780.27694730588711763No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25080.2694274023137669No Hit
GTCCCAGACCCACTGGCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT24740.26577487772099656No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24150.2594366732806009No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23880.25653613904516565No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23300.25030536179867496No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTCTTAGAAG22940.24643798281809462No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22400.24063691434722403No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC21810.23429870990682838No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21780.23397642832511337No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21750.23365414674339832No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20830.22377084490413732No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG20810.22355599051632732No Hit
GCAGAAGCCTGTCCAGGCTCCCAGGCTCCTCATCTATACTACATCCAGCAGGGCCACTGGCATCCCAGACAGGTT20320.2182920580149818No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC19680.21141671760506112No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19610.21066472724772606No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC19410.20851618336962582No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19150.20572307632809556No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA18860.20260768770485024No Hit
GCATTATGGTAGCTCACCGCGCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT18590.19970715346941492No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18550.1992774446937949No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT17560.18864215249719882No Hit
ATGTAGTATAGATGAGGAGCCTGGGAGCCTGGACAGGCTTCTGCTGATACCAGGCTAAGTAGCTGCTTCTAAGA17190.18466734632271345No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCATTATGGTAGCTCA17090.18359307438366332No Hit
GAGTGAAGTCTGTCCCAGACCCACTGGCACTGAACCTGTCTGGGATGCCA16420.1763954523920276No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16290.17499889887126246No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16040.1723132190236372No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15420.16565273300152655No Hit
GGCTTCTGCTGATACCAGGCTAAGTAGCTGCTTCTAAGACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCC15090.16210763560266117No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTC15020.1613556452453261No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14700.15791797504036575No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14510.15587685835617054No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14500.15576943116226555No Hit
GTCTTAGAAGCAGCTACTTAGCCTGGTATCAGCAGAAGCCTGTCCAGGCTCCCAGGCTCCTCATCTATACTACAT14250.1530837513146403No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTC13990.15029064427311No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13890.1492163723340599No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13160.1413741871789941No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13050.140192488046039No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12990.13954792488260892No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT12700.13643253625936363No Hit
GTGCCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTA12560.13492855554469346No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12520.13449884676907342No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12390.1331022932483083No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA12070.12966462304334794No Hit
CCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCAC11850.12730122477743772No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11520.12375612737857236No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11320.12160758350047214No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT11310.12150015630656714No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC11200.12031845717361202No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC11110.11935161242846691No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA11090.1191367580406569No Hit
GTCCAGGCTCCCAGGCTCCTCATCTATACTACATCCAGCAGGGCCACTGG11060.11881447645894186No Hit
ACCATAATGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGC10950.11763277732598675No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC10820.11623622380522161No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG10770.11569908783569656No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC10720.1151619518661715No Hit
CTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGACAAAGACAGGGTGCCTGG10690.11483967028445648No Hit
CTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCA10680.11473224309055145No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC10660.11451738870274145No Hit
GAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTCTTAGAAGCAGCTACTTA10530.11312083518197628No Hit
CTCCCAGGCTCCTCATCTATACTACATCCAGCAGGGCCACTGGCATCCCA10500.11279855360026127No Hit
CCATAATGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCT10440.11215399043683119No Hit
GCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTCCAGGCACC10390.11161685446730615No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC10370.11140200007949612No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG10340.1110797184977811No Hit
GAGCTACCATAATGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGT10250.110112873752636No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA10250.110112873752636No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT10250.110112873752636No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT10210.10968316497701595No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT10210.10968316497701595No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA10040.10785690268063078No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC10040.10785690268063078No Hit
GTTCAGTGCCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGAC10000.10742719390501074No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC9980.1072123395172007No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC9770.10495636844519549No Hit
CACTGGCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCTGGATGTAGTATAGATGAGGAGCCTGGGAGCCTGGA9740.10463408686348044No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC9720.10441923247567042No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC9610.10323753334271532No Hit
GGTTCAGTGCCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGA9420.10119641665852011No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC9350.10044442630118502No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)