FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691738

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691738
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences930863
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCATTATGGTAGCTCACCGCGCACTTTCGGCGGAGGGA62690.6734610785905123No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT51540.5536797573864253No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGGCA43610.4684899926197518No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA36780.3951172191826295No Hit
CATCTATACTACATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGCCAGTGGGTCTGGGACAGACTTCA34750.3733094988199123No Hit
GTGTATTACTGTCAGCATTATGGTAGCTCACCGCGCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA33390.3586994004488308No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33160.35622857498901556No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC32270.3466675547314696No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28980.3113240079367211No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28550.3067046385988056No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28400.3050932306902304No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT27780.2984327446681198No Hit
CTCCTGCAGGGCCAGTCAGAGTCTTAGAAGCAGCTACTTAGCCTGGTATC26250.2819963840006532No Hit
ATACTACATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGCCAGTGGGTCTGGGACAGACTTCACTCTCA25880.27802157782616777No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGGCA25010.26867541195643185No Hit
GTATAGATGAGGAGCCTGGGAGCCTGGACAGGCTTCTGCTGATACCAGGCTAAGTAGCTGCTTCTAAGACTCTGA24930.2678159944051917No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTCTTAGAAG23160.24880138108400485No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23140.2485865266961948No Hit
GTCCCAGACCCACTGGCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT22130.23773638011178871No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21800.2341912827129234No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21750.23365414674339832No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC21660.23268730199825322No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21540.23139817567139312No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20990.22548968000661754No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20860.2240931264858524No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG20190.21689550449421666No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20140.21635836852469162No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA20140.21635836852469162No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19940.21420982464659138No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19310.20744191143057572No Hit
GCAGAAGCCTGTCCAGGCTCCCAGGCTCCTCATCTATACTACATCCAGCAGGGCCACTGGCATCCCAGACAGGTT19170.20593793071590558No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19170.20593793071590558No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18290.19648433765226464No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18100.1944432209680694No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC17800.1912204051509191No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17730.19046841479358403No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCATTATGGTAGCTCA17380.18670846300690866No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC17300.18584904545566858No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTC17300.18584904545566858No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT17150.1842376375470934No Hit
GCATTATGGTAGCTCACCGCGCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT17090.18359307438366332No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT16060.1725280734114472No Hit
ATGTAGTATAGATGAGGAGCCTGGGAGCCTGGACAGGCTTCTGCTGATACCAGGCTAAGTAGCTGCTTCTAAGA16010.17199093744192218No Hit
GTCTTAGAAGCAGCTACTTAGCCTGGTATCAGCAGAAGCCTGTCCAGGCTCCCAGGCTCCTCATCTATACTACAT15750.16919783040039188No Hit
GAGTGAAGTCTGTCCCAGACCCACTGGCACTGAACCTGTCTGGGATGCCA15720.16887554881867686No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTC15230.16361161631733134No Hit
GGCTTCTGCTGATACCAGGCTAAGTAGCTGCTTCTAAGACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCC14990.1610333636636111No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14740.1583476838159858No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14200.1525466153451152No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14180.15233176095730522No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14120.15168719779387513No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13570.14577870212909955No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13460.14459700299614442No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13080.14051476962775403No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA12920.13879593452527386No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12820.13772166258622376No Hit
GTGCCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTA12750.13696967222888867No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11700.12568981686886255No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11420.12268185543952224No Hit
GTCCAGGCTCCCAGGCTCCTCATCTATACTACATCCAGCAGGGCCACTGG11310.12150015630656714No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11170.11999617559189699No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11130.11956646681627695No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC10930.11741792293817672No Hit
CTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGACAAAGACAGGGTGCCTGG10920.11731049574427173No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC10890.11698821416255668No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC10890.11698821416255668No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10880.11688078696865166No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA10690.11483967028445648No Hit
CCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCAC10670.11462481589664644No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC10650.11440996150883644No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC10600.11387282553931137No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA10580.11365797115150135No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT10490.11269112640635624No Hit
ACCATAATGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGC10290.11054258252825604No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC10230.10989801936482597No Hit
GTTCAGTGCCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGAC10110.10860889303796585No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC10070.10817918426234581No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC10060.10807175706844079No Hit
GAGCTACCATAATGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGT9990.10731976671110571No Hit
GAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTCTTAGAAGCAGCTACTTA9980.1072123395172007No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT9920.10656777635377065No Hit
CTCCCAGGCTCCTCATCTATACTACATCCAGCAGGGCCACTGGCATCCCA9910.10646034915986562No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC9850.10581578599643557No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT9840.10570835880253056No Hit
GCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTCCAGGCACC9760.10484894125129048No Hit
CTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCA9700.1042043780878604No Hit
CCATAATGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCT9670.10388209650614537No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC9620.10334496053662032No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA9490.1019484070158552No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT9350.10044442630118502No Hit
GGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTCTTAGAAGCAGCTACTTAGCCTGGTATCAGCAGAAG9350.10044442630118502No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTACG300.002418324168.5371970
GTATCAA18100.040.7588541
CTATTTA558.438439E-636.9175261
AACTGTG25500.036.28439770
TATCAAC20800.034.643382
ATCAACG21250.034.3707283
TCAACGC21550.034.0454944
CAACGCA21600.033.9666865
AACGCAG22050.033.273496
ACGCAGA22800.032.1789677
CGCAGAG23250.031.5561498
TGCGCTA652.6645965E-531.2093775
AGAGTAC23650.031.0224311
GCAGAGT24400.030.068879
AAAACCG1404.026742E-529.37308370
TATGCCG606.1013765E-428.1751310
ATGGGAC500.00708851628.09726550
TCCGCAC855.0750514E-627.91715625
TGTTAGA12350.027.19289470
TAGTTCG500.0085350727.048137
Produced by FastQC (version 0.11.5)