FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691739

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691739
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1180928
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC44210.37436659982657705No Hit
CTCCTGGACCCCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGCCCTGACACTGGCAGGTGTGCAGC42530.360140499674832No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG42270.3579388413180143No Hit
GACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCGCGCTGGTACCACCATAGTAGAGCAGGCAGTAATACT40430.34235787448515065No Hit
GCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCGCGCTGGTACCACCATAGTAGAGCAGG39870.3376158411012356No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG39020.3304181118577932No Hit
GTAATACACAGCCGTGTCCTCAACTCTCAGACTACTGATCTGAAGATACA36590.3098410741383048No Hit
GTCCAGGAGTGTTTGCTGCTTGTACCATAAATCAGTGCCCTGGGTGCTTG35830.3034054574029916No Hit
CCATAGTAGAGCAGGCAGTAATACTCAGCCTCGTCCTCAGGCTGCACACCTGCCAGTGTCAGGGCAGCTTTGCCC35730.30255866572729245No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCA35490.3005263657056146No Hit
GAGTATTACTGCCTGCTCTACTATGGTGGTACCAGCGCGGTGTTCGGCGGAGGGACCAAGTTGACCGTCCTAGGT33560.28418328636462176No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT32950.27901785714285715No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCT32680.27673151961846953No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA31330.2652998319965315No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC30900.26165862779102533No Hit
GTAATACTCAGCCTCGTCCTCAGGCTGCACACCTGCCAGTGTCAGGGCAG30480.2581021027530891No Hit
GTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGACAGTCAC30250.25615448189898116No Hit
CTACTATGGTGGTACCAGCGCGGTGTTCGGCGGAGGGACCAAGTTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC29420.24912611099067852No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT29130.24667041513115107No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT29080.24624701929330156No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG28470.24108159007153696No Hit
GTATTACTGCCTGCTCTACTATGGTGGTACCAGCGCGGTGTTCGGCGGAG28140.2382871775417299No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTC27880.2360855191849122No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC27640.23405321916323435No Hit
GATTTATGGTACAAGCAGCAAACACTCCTGGACCCCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGC26770.22668613158465206No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG25320.21440765228701494No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG25300.21423829395187513No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA24860.21051241057879908No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG24470.20720992304357252No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC24310.20585505636245396No Hit
GTGTATTACTGTGTGAAAGATCGCGGCTCTGTACGTAGAGACTTTGACTA24210.20500826468675482No Hit
CAGTAATACTCAGCCTCGTCCTCAGGCTGCACACCTGCCAGTGTCAGGGCAGCTTTGCCCCCAAGGAGGGAGC23900.2023832104920876No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG23020.1949314437459354No Hit
GTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGCCCTGACACTGGCAGGTG22910.19399997290266638No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC22360.18934261868632127No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21880.18527801864296553No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT21480.18189085194016907No Hit
CTCACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGCTGCCAGCACTGGAGCAGTCACCAGTGATTA21280.18019726858877086No Hit
TCCTCAGGCTGCACACCTGCCAGTGTCAGGGCAGCTTTGCCCCCAAGGAGGGAGCCTGAGAACCGGGCAGGGGTC21030.17808028939952308No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA20420.1729148601777585No Hit
CACCTGTGCTGCCAGCACTGGAGCAGTCACCAGTGATTACTATCCAAACT19740.16715667678300455No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA19600.16597116843702578No Hit
GCACTGGAGCAGTCACCAGTGATTACTATCCAAACTGGTTCCAGCAGAAA19400.1642775850856276No Hit
GCCTGAGGACGAGGCTGAGTATTACTGCCTGCTCTACTATGGTGGTACCA19140.16207592672880988No Hit
GTCCAATTCTCAGACTGTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCAC18810.15928151419900283No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC18360.15547095165835684No Hit
GTGTTCGGCGGAGGGACCAAGTTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACC18340.155301593323217No Hit
CACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGCTGCCAGC18280.15479351831779753No Hit
CAGTAATACACAGCCGTGTCCTCAACTCTCAGACTACTGATCTGAAGATA17950.15199910578799047No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC17710.1499668057663126No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA17500.14818854324734448No Hit
CCATAAATCAGTGCCCTGGGTGCTTGTCCAGGTTTCTGCTGGAACCAGTTTGGATAGTAATCACTGGTGACTGCT17140.14514009321482765No Hit
CCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCAATTCTCAGAC17090.14471669737697812No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT16440.1392125514849339No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC16320.13819640147409495No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT16320.13819640147409495No Hit
ACTATGGTGGTACCAGCGCGGTGTTCGGCGGAGGGACCAAGTTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCT16320.13819640147409495No Hit
AGTACATACTACGCAGACTCCGTGAAGGGCAGATTCACCATCTCCAGAGACAATTCCAAGAACACGCTGTATCTT16010.13557134727942768No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA16010.13557134727942768No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT15890.13455519726858878No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC15810.1338777639280295No Hit
ATCCAAACTGGTTCCAGCAGAAACCTGGACAAGCACCCAGGGCACTGATTTATGGTACAAGCAGCAAACACTC15570.13184546390635163No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACAC15270.1293050888792543No Hit
ACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCGCGCTGGTACCAC15230.12896637220897464No Hit
GGTACCACCATAGTAGAGCAGGCAGTAATACTCAGCCTCGTCCTCAGGCTGCACACCTGCCAGTGTCAGGGCAG14970.12676471385215693No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC14810.12540984717103837No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14770.12507113050075871No Hit
CTCTCACCTGTGCTGCCAGCACTGGAGCAGTCACCAGTGATTACTATCCA14590.12354690548450033No Hit
GTACCTATGCTATGCACTGGGTCCGCCAGGCTCCAGGGAAGGGACTGGAA14550.12320818881422069No Hit
CCTGAGAACCGGGCAGGGGTCCAGGAGTGTTTGCTGCTTGTACCATAAAT14510.12286947214394103No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT14460.12244607630609147No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA14390.1218533221331021No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC14260.12075249295469327No Hit
GTGTGCAGCCTGAGGACGAGGCTGAGTATTACTGCCTGCTCTACTATGGT14150.11982102211142423No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG14140.11973634294385432No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG14060.11905890960329504No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG14000.11855083459787556No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC13940.1180427595924561No Hit
GTATGTACTACCCCCATTACCACCAATACCTGAAACATATTCCAGTCCCTTCCCTGGAGCCTGGCGGACCCAGTG13780.11668789291133753No Hit
GCCGAACACCGCGCTGGTACCACCATAGTAGAGCAGGCAGTAATACTCAGCCTCGTCCTCAGGCTGCACACCTGC13500.11431687621938003No Hit
GGCTGCACACCTGCCAGTGTCAGGGCAGCTTTGCCCCCAAGGAGGGAGCC13260.11228457619770216No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC13220.11194585952742249No Hit
GTATTACTGTGTGAAAGATCGCGGCTCTGTACGTAGAGACTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGT13210.11186118035985258No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT13050.11050631367873402No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC12740.10788125948406678No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT12670.10728850531107738No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC12470.10559492195967918No Hit
TTCTCAGACTGTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGCTGC12280.10398601777585086No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG12240.10364730110557122No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC11910.10085288857576413No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10600.047.8052441
TAGGGGT306.561847E-445.6383254
TCTGGAG13850.040.0047570
GTATCAA28500.037.6027151
GACAGGT1650.037.351521
GTGGTAT2550.036.252941
TACACAT853.2068783E-936.24225
TGGTATC2600.035.5452352
TATCAAC31100.034.4488682
CTGTACT601.4171454E-534.2287454
TCAACGC32150.033.4302524
ATCAACG32200.033.378343
CAACGCA32800.032.8721165
AACGCAG33050.032.6234636
ACGAGAC851.2947021E-732.21665222
ATCTCCG851.295175E-732.2152910
GAGTTTA2900.031.877591
CGCAGAG34500.031.1531168
CACGAGA902.1374035E-730.42683821
AGAGTAC35300.030.25316811
Produced by FastQC (version 0.11.5)