FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004691740

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691740
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1180928
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGACCCCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGCCCTGACACTGGCAGGTGTGCAGC42580.3605638955126816No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC41860.3544669954476479No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG41600.35226533709083024No Hit
GCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCGCGCTGGTACCACCATAGTAGAGCAGG38150.3230510242792109No Hit
GACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCGCGCTGGTACCACCATAGTAGAGCAGGCAGTAATACT37200.31500650336006936No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG37160.31466778668978973No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCA36930.3127201658356818No Hit
GAGTATTACTGCCTGCTCTACTATGGTGGTACCAGCGCGGTGTTCGGCGGAGGGACCAAGTTGACCGTCCTAGGT35330.29917149902449597No Hit
GTAATACACAGCCGTGTCCTCAACTCTCAGACTACTGATCTGAAGATACA33970.28765513223498806No Hit
GTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGACAGTCAC33960.2875704530674182No Hit
GTCCAGGAGTGTTTGCTGCTTGTACCATAAATCAGTGCCCTGGGTGCTTG33960.2875704530674182No Hit
CCATAGTAGAGCAGGCAGTAATACTCAGCCTCGTCCTCAGGCTGCACACCTGCCAGTGTCAGGGCAGCTTTGCCC31630.2678402070236289No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCT31080.26318285280728376No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC30270.25632384023412097No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG29830.2525979568610449No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT29690.2514124485150661No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT28470.24108159007153696No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA28470.24108159007153696No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG28210.23887993171471927No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT28120.2381178192065901No Hit
GTATTACTGCCTGCTCTACTATGGTGGTACCAGCGCGGTGTTCGGCGGAG27130.22973458161716886No Hit
CTACTATGGTGGTACCAGCGCGGTGTTCGGCGGAGGGACCAAGTTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC26900.2277869607630609No Hit
GTAATACTCAGCCTCGTCCTCAGGCTGCACACCTGCCAGTGTCAGGGCAG26740.22643209408194234No Hit
GATTTATGGTACAAGCAGCAAACACTCCTGGACCCCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGC26540.22473851073054413No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC26360.2232142857142857No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTC25130.21279874810318664No Hit
GTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTGCCCTGACACTGGCAGGTG23690.20060494797311945No Hit
GTGTATTACTGTGTGAAAGATCGCGGCTCTGTACGTAGAGACTTTGACTA23690.20060494797311945No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG23010.19484676457836547No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC22890.19383061456752657No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22830.1933225395621071No Hit
CAGTAATACTCAGCCTCGTCCTCAGGCTGCACACCTGCCAGTGTCAGGGCAGCTTTGCCCCCAAGGAGGGAGC22650.1917983145458487No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG22170.18773371450249296No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA22100.1871409603295036No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG21560.18256828528072838No Hit
CTCACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGCTGCCAGCACTGGAGCAGTCACCAGTGATTA21090.17858836440494255No Hit
GTCCAATTCTCAGACTGTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCAC19480.16495501842618687No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA19430.1645316225883373No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19410.1643622642531975No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACAC19370.16402354758291784No Hit
CACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGCTGCCAGC19260.16309207673964882No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA18920.16021298504227185No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC18860.15970491003685236No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT18750.15877343919358336No Hit
GTGTTCGGCGGAGGGACCAAGTTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACC18610.1575879308476046No Hit
GCCTGAGGACGAGGCTGAGTATTACTGCCTGCTCTACTATGGTGGTACCA18280.15479351831779753No Hit
CACCTGTGCTGCCAGCACTGGAGCAGTCACCAGTGATTACTATCCAAACT18010.1525071807934099No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA17440.147680468241925No Hit
CAGTAATACACAGCCGTGTCCTCAACTCTCAGACTACTGATCTGAAGATA17420.14751110990678518No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC17390.14725707240407543No Hit
GCACTGGAGCAGTCACCAGTGATTACTATCCAAACTGGTTCCAGCAGAAA17150.14522477238239756No Hit
TCCTCAGGCTGCACACCTGCCAGTGTCAGGGCAGCTTTGCCCCCAAGGAGGGAGCCTGAGAACCGGGCAGGGGTC16940.14344650986342944No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC16740.14175292651203122No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC16630.1408214556687622No Hit
CTCTCACCTGTGCTGCCAGCACTGGAGCAGTCACCAGTGATTACTATCCA16520.13988998482549317No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT16370.13861979731194451No Hit
CCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCAATTCTCAGAC16340.13836575980923477No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA16060.13599474311727727No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT15930.1348939139388684No Hit
GTGTGCAGCCTGAGGACGAGGCTGAGTATTACTGCCTGCTCTACTATGGT15490.13116803056579232No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC15350.12998252221981357No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC14900.12617195967916756No Hit
ACTATGGTGGTACCAGCGCGGTGTTCGGCGGAGGGACCAAGTTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCT14880.12600260134402774No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT14870.12591792217645784No Hit
CCATAAATCAGTGCCCTGGGTGCTTGTCCAGGTTTCTGCTGGAACCAGTTTGGATAGTAATCACTGGTGACTGCT14710.12456305549533926No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC14590.12354690548450033No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC14480.1226154346412313No Hit
GTACCTATGCTATGCACTGGGTCCGCCAGGCTCCAGGGAAGGGACTGGAA14300.12109120962497291No Hit
AGTACATACTACGCAGACTCCGTGAAGGGCAGATTCACCATCTCCAGAGACAATTCCAAGAACACGCTGTATCTT14200.12024441794927379No Hit
ATCCAAACTGGTTCCAGCAGAAACCTGGACAAGCACCCAGGGCACTGATTTATGGTACAAGCAGCAAACACTC14150.11982102211142423No Hit
GGCTGCACACCTGCCAGTGTCAGGGCAGCTTTGCCCCCAAGGAGGGAGCC14090.11931294710600478No Hit
ACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCGCGCTGGTACCAC13600.11516366789507912No Hit
GGTACCACCATAGTAGAGCAGGCAGTAATACTCAGCCTCGTCCTCAGGCTGCACACCTGCCAGTGTCAGGGCAG13460.11397815954910036No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT13430.11372412204639065No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG13380.11330072620854108No Hit
CCTGAGAACCGGGCAGGGGTCCAGGAGTGTTTGCTGCTTGTACCATAAAT13120.1110990678517234No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT12910.10932080533275525No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA12890.10915144699761543No Hit
GTATGTACTACCCCCATTACCACCAATACCTGAAACATATTCCAGTCCCTTCCCTGGAGCCTGGCGGACCCAGTG12870.1089820886624756No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC12770.10813529698677649No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12540.10618767613266855No Hit
GTATTACTGTGTGAAAGATCGCGGCTCTGTACGTAGAGACTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGT12520.10601831779752872No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG12410.1050868469542597No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG12400.1050021677866898No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG12350.10457877194884023No Hit
GCCGAACACCGCGCTGGTACCACCATAGTAGAGCAGGCAGTAATACTCAGCCTCGTCCTCAGGCTGCACACCTGC12230.10356262193800131No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12000.10161500108389335No Hit
TTCTCAGACTGTGGTGACTCAGGAGCCCTCACTGACTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGCTGC11870.10051417190548449No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)