FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691741

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691741
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences716073
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT26710.3730066627285207No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC25740.35946055779229213No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT22900.31979979694807653No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT21540.30080731992408594No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG15300.21366536651989393No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG15220.21254816198907092No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC13410.18727140947920115No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT11060.15445352638627627No Hit
GAAGACCATCGACCGCTTGGCGGGTAAACCCACCCATGTCAATGTGTCTGTTGTCATGGCGGAGGTGGACGGCAC10230.14286252937898788No Hit
CCAGCATACTGCGCGTGGCAGCCGAGGACTGGAAGAAGGGGGACACCTTCTCCTGCATGGTGGGCCACGAGGCC9870.1378351089902845No Hit
CTCCTGCATGGTGGGCCACGAGGCCCTGCCGCTGGCCTTCACACAGAAGACCATCGACCGCTTGGCGGGTAAACC9510.13280768860158113No Hit
CCTTCACACAGAAGACCATCGACCGCTTGGCGGGTAAACCCACCCATGTC8770.1224735466914686No Hit
GGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAA8710.12163564329335137No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8510.11884263196629394No Hit
GCATGGAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGT8390.11716682517005947No Hit
ATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGCCCACCATGCA8270.11549101837382501No Hit
GGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCTCCGCC8250.11521171724111928No Hit
GCATACTGCGCGTGGCAGCCGAGGACTGGAAGAAGGGGGACACCTTCTCCTGCATGGTGGGCCACGAGGCCCTGC7790.10878779118888716No Hit
GACCAGCATACTGCGCGTGGCAGCCGAGGACTGGAAGAAGGGGGACACCTTCTCCTGCATGGTGGGCCACGAGGC7470.10431897306559527No Hit
GGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCTCCGCCAT7410.10348106966747803No Hit
GTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCG7280.10166561230489071No Hit
CCTTCGCTGTGACCAGCATACTGCGCGTGGCAGCCGAGGACTGGAAGAAG7180.10026910664136199No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAT150.002434924268.4225839
CGCTTAT253.5651692E-451.6454476
TCGTATG4300.045.68802640
CGTATGC4500.044.0019841
CTCGTAT4150.043.6915239
ACCGTAC4500.041.88997727
GCCGTCT4950.041.8776146
TATACAC4650.041.6495553
GTACTAG4700.041.21335230
GTATGCC4850.041.1326442
ACGAGAC4600.040.71592322
CCCACGA4600.040.70170619
ATGCCGT5000.040.30149544
TAGATCT4500.039.94766634
TCTCCGA4950.039.77465411
CGTCTTC5200.039.66152248
ATCTCCG4750.039.4081310
ATCTCGT4650.039.1725337
GACCGTA4850.038.77972426
AAAGGGG4600.038.56543768
Produced by FastQC (version 0.11.5)