FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691744

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691744
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1730871
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGGTAGCTCACCGTGGACGTTCGGCCAAGGGA66680.38523957013549825No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT54400.3142926307044257No Hit
GTAATATACGGCCGTGTCCGCAGAGGTCACAGACTGCAGCTTCAGGGAGA53400.3085151926400061No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG46230.26709096171811764No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCTACTGCCA45700.2640289195439753No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC39280.22693776717040148No Hit
GTATGGTAGCTCACCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT36800.2126097207706409No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA36800.2126097207706409No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC35580.205561246332049No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG35300.2039435636740115No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC33990.19637511980962188No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT32870.1899043891774719No Hit
GTGTATTACTGTCAGCAGTATGGTAGCTCACCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA32490.18770896271299248No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32300.18661124948075275No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT29200.16870119148105203No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTACTTAGCCTGGTACC28690.16575469806819804No Hit
GTCTCTGGTGGCTCCATCAGTACTTACTACTGGAGCTGGATCCGGCAGCC28130.16251933275212307No Hit
GTCCCAGACCTACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT27810.1606705525715088No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27610.15951506495862489No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27360.15807070544251997No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA27340.15795515668123158No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG27320.1578396079199432No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTAGGTCTGGGACAGACTTCA26140.1510222310039281No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT25720.14859570701687186No Hit
CTGCTATACGACCCCCCTCCTCTCGCACAGTAATATACGGCCGTGTCCGC25400.14674692683625756No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG24750.14299159209438483No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC24520.14166278133956833No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCTACTGCCA23860.1378496722170514No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23610.13640531270094652No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC23570.13617421517836972No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23080.13334327052680414No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC23070.13328549614615995No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22260.12860577131398007No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21880.12641034484950062No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTTTGCTGGTACCAGGCTAAGTAGTTGCTGCTAACACTCTGA21860.12629479608821223No Hit
CTACCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC21550.12450379028824217No Hit
GGGTATATGTTTCACAATGACGGCTCCATCTACAACCCCTCCCTCAAGAG21430.12381049772051181No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21220.12259723572698371No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT21210.1225394613463395No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21170.12230836382376273No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC20880.12063290678508104No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC20440.11809083403673641No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT19890.11491324310130564No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC19820.11450882243679626No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA19770.11421995053357528No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19680.11369998110777753No Hit
GAGTGAAGTCTGTCCCAGACCTACTGCCACTGAACCTGTCTGGGATGCCA19440.11231339597231682No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT19290.11144678026265388No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT18640.10769144552078115No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGGTAGCTCA18150.10486050086921554No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17440.10075851984347765No Hit
GTGTTAGCAGCAACTACTTAGCCTGGTACCAGCAAAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCAT17400.10052742232090085No Hit
GTCAGAGTGTTAGCAGCAACTACTTAGCCTGGTACCAGCAAAAACCTGGC17360.1002963247983241No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14450.038.9631921
TGCTATA6350.037.6716042
GCTATAC6650.036.96943
CTATACG6400.036.8348774
GTATCAA36350.036.7279471
ATACGAC6500.035.7521366
CGATCGC601.5769405E-533.70000519
TATCAAC39450.033.3078882
AACTGTG30650.033.01804770
ACGACCC7300.032.2945258
TGTTAGA15000.032.16963670
CGACCCC7400.031.856279
ATCAACG41500.031.8111323
TCAACGC41200.031.797544
CAACGCA41750.031.4602265
TACGACC7400.031.4039047
TAGTCGG553.624152E-430.79849430
AACGCAG42550.030.790476
AATGGTA9350.030.10527470
GTCGTAG450.00517515529.9676951
Produced by FastQC (version 0.11.5)