FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691746

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691746
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1525245
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC45320.2971325918131186No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG36350.23832236788188127No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG33910.22232493796078664No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG33850.22193155853649743No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG33760.2213414894000636No Hit
GTGCTGGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC31790.2084255316359011No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCCAGCACTCAGGCTGGTCTCCCATGAT30420.19944336811463076No Hit
GTAATACATGGCCGTGTCCGCAGCGGTCACAGAGGTCAGCTTCAGGGAGA30350.19898442545296002No Hit
GTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAGGGTCACCATCTCCTG30150.19767316070532934No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC30080.19721421804365855No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT29880.19590295329602786No Hit
CCCCAGCACTCAGGCTGGTCTCCCATGATCCGCAGTAATAATCGGCCTCGTCCCCTGTCTGGAGTCCGGTGATGC29460.19314929732600336No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA27520.1804300292739855No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG27110.17774193654134254No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCCAGCACTCAGG26490.17367701582368733No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT25720.16862864654530912No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG25650.1681697038836384No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC25620.16797301417149377No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT24880.16312133460526013No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA24480.16049880510999873No Hit
GTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAGGGTCAC23110.15151664158872838No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG22770.14928749151775617No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22680.14869742238132233No Hit
GTCTCTGGTGGCTCCATCAGTAGTTACTACTGGAGTTGGGTCCGGCAGCT22520.1476484105832178No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC22310.14627158259820555No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21680.1421410986431688No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT21670.14207553540578727No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC21220.13912518972361818No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC21160.13873181029932896No Hit
CCCAGGAACAGCCCCCAAACTCCTCATCTATGAAAATAATAAGCGACCCTCAGGGATTCCTGACCGATTCTCTGG20110.13184767037426776No Hit
CAGTAATACATGGCCGTGTCCGCAGCGGTCACAGAGGTCAGCTTCAGGGA19920.13060196886401856No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG19360.1269304275706526No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT19020.12470127749968038No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC18950.12424233483800963No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA18850.12358670246419429No Hit
GTCTGGAGTCCGGTGATGCCCAGGGTGGCTGACGTGCCAGACTTGGAGCC18660.12234100095394511No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC18610.12201318476703742No Hit
GGACAGAGGGTCACCATCTCCTGCTCTGGAAGCAGCTCCAACATTGGGATTAATTTTGTAGCCTGGTACCAGCAG18540.12155424210536668No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC18010.1180793905241453No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC18000.11801382728676377No Hit
GATTATTACTGCGGATCATGGGAGACCAGCCTGAGTGCTGGGGTGTTCGG17480.11460453894292394No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT17370.11388334333172705No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC16650.10916279024025648No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA16530.10837603139167806No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG16330.10706476664404735No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT16170.10601575484594278No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG16070.10536012247212743No Hit
ATGTATTACTGTGCGAGGCGTCACAGCACCGGAGAATTAGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTC15900.10424554743664131No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT15460.10136076499185377No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT15300.10031175319374921No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13150.043.2195051
GTATCAA37100.038.9855881
TGGTATC4150.036.882932
GTGGTAT4350.035.9879951
TATCAAC40650.035.3112342
TAATGAT11500.034.18152670
CAATAGT400.002788023634.00314
AATGGTA7650.033.96568770
ATCAACG42750.033.6451723
TCAACGC42900.033.6067964
CAACGCA43600.033.0694055
AACGCAG44550.032.364226
GGATGGG14950.032.0869670
CGCAGAG46650.030.907318
ACGCAGA46700.030.8742187
TACGTAT1103.9981207E-430.28409670
ACAGACG8150.030.24693770
AGAGTAC47650.030.11298411
GCAGAGT48150.030.0121369
CAGAGTA48050.029.9330710
Produced by FastQC (version 0.11.5)