FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004691747

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691747
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences779777
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATACTCCTCCCCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT45160.5791399335963999No Hit
GTATAATATTGTTGACAGTAATAAACTGCCACATCTTCAGCCTGAAGGCTGCTGATGGAGAGAGTGAAATCTGTC31960.4098607678862034No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25860.33163327464133974No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT25310.3245799760700816No Hit
CTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT23830.3056001908237868No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG20070.25738127695482166No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19030.24404413056553345No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18660.23929918425395977No Hit
GCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGGTCTTGCTGTTGGAGCTATATGAAAGACT18080.2318611603060875No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17860.22903984087758425No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17370.2227559930595542No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16510.21172719892995048No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15550.19941598687829984No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15340.19672290924200123No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14440.18518114794357873No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13770.1765889478658642No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13070.16761202241153558No Hit
CTTTCATATAGCTCCAACAGCAAGACCTACTTAGCTTGGTACCAGCAGAA12760.16363652685319008No Hit
GTAATAAACTGCCACATCTTCAGCCTGAAGGCTGCTGATGGAGAGAGTGA12560.16107169100909619No Hit
GTTTATTACTGTCAACAATATTATACTCCTCCCCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA12450.15966103129484455No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT12040.15440311781445207No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11810.1514535565937441No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11650.149401687918469No Hit
CTCCATCAGCAGCCTTCAGGCTGAAGATGTGGCAGTTTATTACTGTCAACAATATTATACTCCTCCCCTCACTTT11630.14914520433405962No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11350.14555443415232816No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG10680.13696223407461364No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG10560.1354233325681573No Hit
AACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAA10380.1331149803084728No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10250.13144783700981177No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9690.12426629664634889No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG9670.1240098130619395No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9600.12311212051650665No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9570.12272739513989256No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG9460.12131673542564092No Hit
GTCCCTGACCGATTCATTGGCAGCGGGTCTGGGACAGATTTCACTCTCTC9440.12106025184123154No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9360.12003431750359397No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT9290.11913662495816112No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC8960.11490464581540619No Hit
GATTTCACTCTCTCCATCAGCAGCCTTCAGGCTGAAGATGTGGCAGTTTATTACTGTCAACAATATTATACTCCT8770.112468051763517No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8610.11041618308824189No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA8600.11028794129603721No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC8520.10926200695839965No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT8450.10836431441296679No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC8430.1081078308285574No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCTTCAGCTGCAAGTCCAGTCAGAGTCTTTCATATAGCTCCAA8400.10772310545194332No Hit
GTACAACGTGTCCCTGGTCATGTCCGACACAGCTGGCACCTGCTACTGAC8390.10759486365973861No Hit
GCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCATTGGCAGCGGGTCTGGGACAGATTT8340.10695365469871514No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8290.10631244573769166No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG8270.10605596215328228No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCTTCAGCT8050.103234642724779No Hit
ATATGAAAGACTCTGACTGGACTTGCAGCTGAAGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA8040.1031064009325743No Hit
GAGTACGGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACAT8000.10259343376375553No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC7940.10182398301052738No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)