FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691748

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691748
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences779777
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATACTCCTCCCCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT41590.5333576137793241No Hit
GTATAATATTGTTGACAGTAATAAACTGCCACATCTTCAGCCTGAAGGCTGCTGATGGAGAGAGTGAAATCTGTC31090.398703731964395No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27770.3561274569524364No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT24180.3100886535509511No Hit
CTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT22750.2917500772656798No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21360.2739244681492273No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19150.2455830320719898No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18110.2322458856827016No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17280.22160181692971195No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG17140.21980643183884624No Hit
GCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGGTCTTGCTGTTGGAGCTATATGAAAGACT15880.20364796602105475No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15700.20133961376137022No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15440.19800532716404817No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14660.188002467372082No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14440.18518114794357873No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13770.1765889478658642No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13250.1699203746712201No Hit
GTAATAAACTGCCACATCTTCAGCCTGAAGGCTGCTGATGGAGAGAGTGA12950.16607312090507925No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12110.15530081035988494No Hit
GTTTATTACTGTCAACAATATTATACTCCTCCCCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA12060.15465960139886145No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11440.14670861028217042No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11370.14581091773673754No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11250.1442720162302812No Hit
CTTTCATATAGCTCCAACAGCAAGACCTACTTAGCTTGGTACCAGCAGAA11230.14401553264587183No Hit
CTCCATCAGCAGCCTTCAGGCTGAAGATGTGGCAGTTTATTACTGTCAACAATATTATACTCCTCCCCTCACTTT11110.1424766311394155No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG10710.13734695945122774No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10380.1331149803084728No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG10350.13273025493185872No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10330.13247377134744934No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10030.1286265175813085No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA9970.12785706682808035No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9580.12285563693209725No Hit
GTACAACGTGTCCCTGGTCATGTCCGACACAGCTGGCACCTGCTACTGAC9400.12054728467241277No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC9360.12003431750359397No Hit
AACCAGGACAGCCTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAA9250.11862365778934235No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG9220.11823893241272826No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9100.11670003090627193No Hit
GAGTACGGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACAT8870.11375046968556395No Hit
GATTTCACTCTCTCCATCAGCAGCCTTCAGGCTGAAGATGTGGCAGTTTATTACTGTCAACAATATTATACTCCT8860.11362222789335924No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCAATGAATCGGTCAGGGACCCCG8660.11105739204926536No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG8580.11003145771162781No Hit
GTCCCTGACCGATTCATTGGCAGCGGGTCTGGGACAGATTTCACTCTCTC8470.10862079799737617No Hit
GTCCCAGACCCGCTGCCAATGAATCGGTCAGGGACCCCGGATTCCCGGGT8370.10733838007532923No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCTTCAGCTGCAAGTCCAGTCAGAGTCTTTCATATAGCTCCAA8290.10631244573769166No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCTTCAGCT8020.10284991734816491No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC7900.10131101584170858No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG7900.10131101584170858No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC7890.1011827740495039No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA7820.10028508150407103No Hit
GTATCAACGCAGAGTACGGGTGACCACGGGTGACGGGGAATCAGGGTTCG7810.10015683971186634No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTACG150.002713336666.5803220
GCGTATC150.002716101266.5632251
GGTATCA12950.047.2881551
GTGGTAT2600.042.2420431
TGGTATC2900.040.1571462
GTATCAA34150.039.1777651
TATCAAC41350.032.3476942
ATCAACG42150.031.7194863
TCAACGC42450.031.5736664
TCCGAGA1751.6516424E-731.32398870
AACGCAG42850.031.276926
CGGTGAG450.00428983931.1372145
CAACGCA43100.031.0974965
CGAACTA553.8827318E-430.43581229
AGCGAAC553.9240686E-430.38086327
ACGCAGA45000.029.7825837
CGCAGAG45050.029.6757058
AGAGTAC45800.029.1152711
CGAAACC954.6102672E-528.79773969
CAGAGTA46600.028.61543710
Produced by FastQC (version 0.11.5)