FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691751

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691751
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1474007
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC52080.35332260972980456No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT42180.286158749585314No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCATATACCACATGATCACTTCCACTATCCCACAC40760.27652514540297296No Hit
ATCCCACACCTGACAATAATAGTCGGCCTCATCCCCGGCTTCGACCCTGC36830.24986312819409948No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG36590.24823491340271792No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC36380.24681022546025902No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG35420.24029736629473264No Hit
GTATATGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCC34680.23527703735463945No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCATATACCACATGATCACTT33770.22910338960398427No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT32850.22286189957035482No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC29840.20244137239511076No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG28630.19423245615522855No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC28270.19179013396815617No Hit
GCCATATACCACATGATCACTTCCACTATCCCACACCTGACAATAATAGTCGGCCTCATCCCCGGCTTCGACC27880.1891442849321611No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT27550.18690548959401143No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT27530.18676980502806295No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG27490.18649843589616605No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCCCTG27060.18358121772827402No Hit
CCCCTGTCCTGGTCGTCTATAATGATGGCGACCGGCCCTCAGGGATCCCT26730.18134242239012432No Hit
GGCCCTGTGATGTCCTACGTGCTGACTCAGGCACCCTCGGTGTCAGTGGCCCCAGGACAGACGGCCACGATTAC25720.1744903518097268No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG25550.17333703299916486No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG25380.1721837141886029No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC25290.17157313364183482No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT24660.16729906981445816No Hit
CCCCAGGACAGACGGCCACGATTACCTGTGGGGGAAACTACATTGGGAGT24660.16729906981445816No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA24420.16567085502307655No Hit
GTCCTGGTCGTCTATAATGATGGCGACCGGCCCTCAGGGATCCCTGAGCG24230.16438185164656613No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG24080.16336421740195264No Hit
CTTTTACTCCCAATGTAGTTTCCCCCACAGGTAATCGTGGCCGTCTGTCCTGGGGCCACTGACACCGAGGGTGC22850.155019616596122No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG22660.1537306132196116No Hit
GGACAGGGGCCTGGCCTGGCCTCTGCTGGTACCAGTGCACACTTTTACTC22480.1525094521260754No Hit
GAGTAAAAGTGTGCACTGGTACCAGCAGAGGCCAGGCCAGGCCCCTGTCC22400.15196671386228153No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA22260.15101692190064225No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC22120.150067129939003No Hit
GCCCTCAGGGATCCCTGAGCGAATCTCTGGCTCCAACTCTGGGAAAACGG22120.150067129939003No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC21990.14918518026033797No Hit
GGCCAGGCCAGGCCCCTGTCCTGGTCGTCTATAATGATGGCGACCGGCCC21800.14789617688382756No Hit
GTGATGTCCTACGTGCTGACTCAGGCACCCTCGGTGTCAGTGGCCCCAGG21620.14667501579029135No Hit
GTGTGGGATAGTGGAAGTGATCATGTGGTATATGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG21450.1455216969797294No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA20560.13948373379502269No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCCCTGTGATGTCCTACGTGCTGACT19560.1326995054975994No Hit
GGCCAGGCCCCTGTCCTGGTCGTCTATAATGATGGCGACCGGCCCTCAGG19270.13073207929134664No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT18980.12876465308509388No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT18920.1283575993872485No Hit
CTCACTGCACAGGCCCTGTGATGTCCTACGTGCTGACTCAGGCACCCTCGGTGTCAGTGGCCCCAGGACAGACGG18620.1263223308980215No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC18550.12584743491720188No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC18540.12577959263422764No Hit
CTATTATTGTCAGGTGTGGGATAGTGGAAGTGATCATGTGGTATATGGCG18420.12496548523853687No Hit
GCACAGGCCCTGTGATGTCCTACGTGCTGACTCAGGCACCCTCGGTGTCA18200.12347295501310375No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC18160.1232015858812068No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC18120.12293021674930986No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA17850.12109847510900558No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT17450.11838478379003628No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTC17150.11634951530080928No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCATATACCACATGATCACTTC16790.1139071931137369No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG16740.11356798169886574No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA16410.11132918636071606No Hit
CCTCTCTCACTGCACAGGCCCTGTGATGTCCTACGTGCTGACTCAGGCAC16090.10915823330554061No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT15850.10753001851415901No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT15630.10603748828872589No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC15340.10407006208247316No Hit
CAATAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGT14870.10088147478268421No Hit
TCTATAATGATGGCGACCGGCCCTCAGGGATCCCTGAGCGAATCTCTGGCTCCAACTCTGGGAAAACGGCCACC14870.10088147478268421No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT14790.10033873651889036No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATGCG250.001207472881.6527670
AGGGGGG3750.056.24968370
CGTATGC1400.046.79154641
ACTACGC3700.046.41049627
CTACGCT3750.045.84468528
GGTATCA16500.044.207981
TACGCTG4150.042.28280329
TCTCGTA1700.040.24986338
ATGCCGT2550.039.50594744
AGACTAC4350.039.42332525
ACGCTGC4500.039.0273930
GACTACG4400.038.99651726
ATCGCGT801.8226274E-938.40005537
CCCACGA4400.038.18388719
GCATCGC1250.038.09546335
ACGAGAC4450.037.75485222
AACGCTA400.001688545137.6613366
CGCGTAT557.692906E-637.42084539
CGAGACT4500.037.33535423
GTATCAA41100.037.294771
Produced by FastQC (version 0.11.5)