FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004691755

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691755
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences704029
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCTGAGGCCTCCTCTGAGCTGACACAGGCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCAC47920.6806537798869081No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC36950.5248363348668875No Hit
GCCTTGGGCTGACCTAGGACGCTCAGCCTGGTCCCTCCTCCGAACACCCA35510.5043826319654445No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG32260.45821976083371563No Hit
GACCTAGGACGCTCAGCCTGGTCCCTCCTCCGAACACCCAATGATTACCA29060.4127670877193979No Hit
GTGTTGGTCATGTACGAGGACAGCAAACGACCCTCCGGGATCCCTGAGAG28160.3999835234059961No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG27180.3860636422647363No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT23000.32669108800915875No Hit
GTACTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG22820.32413437514647836No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGCTCAGCCTGGTCCCTC21280.30226027621021295No Hit
CTACTACTGTTACTCAACAGACAGTAGTGGTAATCATTGGGTGTTCGGAG19360.2749886723416223No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT19120.27157972185804846No Hit
CCTCTGAGCTGACACAGGCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAAATA18970.2694491278058148No Hit
CCTCAGGGACAATGGCCACCTTGACCATCAGTGGGGCCCAGATGGAAGATGAAGCTGACTACTACTGTTACTCAA18970.2694491278058148No Hit
CTTTCTGCACAGTCTCTGAGGCCTCCTCTGAGCTGACACAGGCACCCTCGGTGTCAGTGTCCCCAGGACAAACGG18920.26873892978840364No Hit
GTAATAAACAGCCGTGTCCTCCGCTCTCAGGCTGTTCATTTGCAGAAACA18570.2637675436665251No Hit
GTCTTCTCCACGGTACTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT17830.25325661300883917No Hit
GTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAAATAC17580.24970562292178305No Hit
GACTACTACTGTTACTCAACAGACAGTAGTGGTAATCATTGGGTGTTCGG17090.24274568235115318No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG16920.240331009091955No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGATCTAGTCTCC16590.235643702177041No Hit
GGTGTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA16150.22939395962382234No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA16100.2286837616064111No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG15560.22101362301837No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC15400.21874098936265407No Hit
CAGTAGTAGTCAGCTTCATCTTCCATCTGGGCCCCACTGATGGTCAAGGT15140.2150479596721158No Hit
CCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAAATACATTGGCAAAA14990.21291736561988212No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC14910.2117810487920242No Hit
GTTTATTACTGTGCGAGAGGAGTTACATACAGTGATGCTGCGGTGACAAC14810.21036065275720178No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT14000.19885544487514012No Hit
GTAGTAGTCAGCTTCATCTTCCATCTGGGCCCCACTGATGGTCAAGGTGG13990.19871340527165782No Hit
CCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCC13980.1985713656681756No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA13970.19842932606469335No Hit
ACACAGGGGCCAGGCCTGACTTCTGCTGATACCAATAAGCATATTTTTTT13950.19814524685772889No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA13080.18578780135477374No Hit
CCCCTGTGTTGGTCATGTACGAGGACAGCAAACGACCCTCCGGGATCCCT13000.1846514845269158No Hit
GAGCTGACACAGGCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAG12780.18152661325030645No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC12530.17797562316325038No Hit
AGTCAGGCCTGGCCCCTGTGTTGGTCATGTACGAGGACAGCAAACGACCC12510.1776915439562859No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA12150.17257811823092514No Hit
TATTTATTGAGGGTGTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG12040.17101568259262048No Hit
GACCAACACAGGGGCCAGGCCTGACTTCTGCTGATACCAATAAGCATATT12040.17101568259262048No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC12000.1704475241786915No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGCTCAGCCTGGTCCCTCCTCCGAACAC11950.1697373261612803No Hit
CCACTACTGTCTGTTGAGTAACAGTAGTAGTCAGCTTCATCTTCCATCTG11890.16888508854038684No Hit
CAGTAGTGGTAATCATTGGGTGTTCGGAGGAGGGACCAGGCTGAGCGTCC11640.16533409845333077No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGATCTAGT11350.1612149499523457No Hit
GCCTGGTCCCTCCTCCGAACACCCAATGATTACCACTACTGTCTGTTGAGTAACAGTAGTAGTCAGCTTCATCTT11220.15936843510707654No Hit
CCCCTGGGATCCTGCAGATCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTACGAACATTCTGTAGGGGC11120.15794803907225413No Hit
GATCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTACGAACATTC11110.15780599946877188No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG11060.15709580145136065No Hit
GGGTGTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG11010.15638560343394944No Hit
GGATCACCTGCTCTGGAAATACATTGGCAAAAAAATATGCTTATTGGTATCAGCAGAAGTCAGGCCTGGCCC10950.155533365813056No Hit
GTAGTGGTAATCATTGGGTGTTCGGAGGAGGGACCAGGCTGAGCGTCCTA10840.15397093017475133No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT10830.15382889057126908No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG10700.15198237572599993No Hit
GGCCTGGCCCCTGTGTTGGTCATGTACGAGGACAGCAAACGACCCTCCGG10570.15013586088073078No Hit
CTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCCTCTGA10560.14999382127724853No Hit
GGACCAGGCTGAGCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG10490.1489995440528728No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA10180.1445963163449233No Hit
CTCTGAGGCCTCCTCTGAGCTGACACAGGCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTG10020.1423236826892074No Hit
GTCCCTGAGGTGGAGCCAGAGAATCTCTCAGGGATCCCGGAGGGTCGTTT9830.13962493022304479No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA9820.13948289061956257No Hit
CCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCTCTGG9600.1363580193429532No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC9490.13479558370464853No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC9400.13351722727330834No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA9350.13280702925589713No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG9220.13096051441062798No Hit
GCAAACGACCCTCCGGGATCCCTGAGAGATTCTCTGGCTCCACCTCAGGG9180.130392355996699No Hit
GGCCTGGACCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTG9140.12982419758277003No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGCTCAGCCTGGTCCCTCCTCCGAACACCCAATGATTAC9140.12982419758277003No Hit
GTATGTAACTCCTCTCGCACAGTAATAAACAGCCGTGTCCTCCGCTCTCA9030.12826176194446537No Hit
CCTACGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTACTCCCTTCA9030.12826176194446537No Hit
GGAAATACATTGGCAAAAAAATATGCTTATTGGTATCAGCAGAAGTCAGG8940.12698340551312517No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC8840.12556300947830273No Hit
GAGGAGGGACCAGGCTGAGCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCG8730.12400057383999807No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTACTCCCTTCATGCGTGACCTGG8710.12371649463303357No Hit
GGCCTGGACCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCCTCTGAGCTGACACAGG8620.1224381382016934No Hit
GTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGAC8610.12229609859821114No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTACTCCCTTCATGCGTGACC8440.11988142533901301No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG8430.11973938573553078No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC8400.11931326692508405No Hit
GTACGAGGACAGCAAACGACCCTCCGGGATCCCTGAGAGATTCTCTGGCT8350.11860306890767285No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC8250.11718267287285042No Hit
GCTCAGGAGGCAGAGCTCTGGGAATCTCACCATGGCCTGGACCCCTCTCC8230.11689859366588592No Hit
CCCAATGATTACCACTACTGTCTGTTGAGTAACAGTAGTAGTCAGCTTCA8230.11689859366588592No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC8210.11661451445892144No Hit
GATCAGCACTGAGCACCGAGGATTCACCATGGACCTGGGGCTCCGCTGGG8000.11363168278579433No Hit
GGCCAGGCCTGACTTCTGCTGATACCAATAAGCATATTTTTTTGCCAATGTATTTCCAGAGCAGGTGATCCTGGC7990.1134896431823121No Hit
CCTTGACCATCAGTGGGGCCCAGATGGAAGATGAAGCTGACTACTACTGT7970.1132055639753476No Hit
GACACAGGCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCA7920.1124953659579364No Hit
GCTCTGGGAATCTCACCATGGCCTGGACCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCT7890.11206924714748966No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT7780.110506811509185No Hit
GGCCTGACTTCTGCTGATACCAATAAGCATATTTTTTTGCCAATGTATTT7700.10937049468132704No Hit
CATTCTGTAGGGGCCACTGTCTTCTCCACGGTACTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGA7680.10908641547436257No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC7640.1085182570604336No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG7520.10681378181864667No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGTA7510.10667174221516444No Hit
GACCATCAGTGGGGCCCAGATGGAAGATGAAGCTGACTACTACTGTTACT7460.10596154419775321No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC7440.10567746499078874No Hit
TGTACGAGGACAGCAAACGACCCTCCGGGATCCCTGAGAGATTCTCTGGC7440.10567746499078874No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT7390.10496726697337752No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG7380.10482522736989527No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG7330.10411502935248405No Hit
CCCCTCCCCTGGGATCCTGCAGATCTAGTCTCCCGTGGTGGGGGGTGAGG7320.10397298974900182No Hit
CAGGAGGCAGAGCTCTGGGAATCTCACCATGGCCTGGACCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGT7260.10312075212810835No Hit
CTCCTGTGCAGCCTCTGGATTCATGTTCACTAGCCATAGTATGAGTTGGATCCGCCAGGCTCCAGGGAAGGGG7170.10184239569676819No Hit
CTGCAGATCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTACGAACATTCTGTAGGGGCCACTGTCTTCT7150.1015583164898037No Hit
CCACGGTACTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGAC7120.10113219767935695No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC7120.10113219767935695No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC7120.10113219767935695No Hit
ATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCC7110.10099015807587472No Hit
GGCCTCCTCTGAGCTGACACAGGCACCCTCGGTGTCAGTGTCCCCAGGAC7080.10056403926542799No Hit
GTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTT7060.1002799600584635No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)