FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004691756

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691756
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences704029
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCTGAGGCCTCCTCTGAGCTGACACAGGCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCAC47750.6782391066277099No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC34650.4921672260659717No Hit
GCCTTGGGCTGACCTAGGACGCTCAGCCTGGTCCCTCCTCCGAACACCCA31270.44415784008897363No Hit
GTGTTGGTCATGTACGAGGACAGCAAACGACCCTCCGGGATCCCTGAGAG29320.4164601174099362No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG29070.4129091273228801No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG28400.40339247388956995No Hit
GACCTAGGACGCTCAGCCTGGTCCCTCCTCCGAACACCCAATGATTACCA24070.3418893255817587No Hit
GTACTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG22840.3244184543534428No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT20570.29217546436297365No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGCTCAGCCTGGTCCCTC19170.2722899198754597No Hit
GTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAAATAC18610.26433570208045404No Hit
CTTTCTGCACAGTCTCTGAGGCCTCCTCTGAGCTGACACAGGCACCCTCGGTGTCAGTGTCCCCAGGACAAACGG18550.2634834644595606No Hit
GGTGTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA17860.25368273181928586No Hit
CTACTACTGTTACTCAACAGACAGTAGTGGTAATCATTGGGTGTTCGGAG17520.2488533853008896No Hit
CCTCTGAGCTGACACAGGCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAAATA17320.24601259323124475No Hit
GTAATAAACAGCCGTGTCCTCCGCTCTCAGGCTGTTCATTTGCAGAAACA17200.2443081179894578No Hit
CCTCAGGGACAATGGCCACCTTGACCATCAGTGGGGCCCAGATGGAAGATGAAGCTGACTACTACTGTTACTCAA17140.24345588036856436No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT17030.2418934447302597No Hit
GTCTTCTCCACGGTACTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT16990.2413252863163307No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGATCTAGTCTCC16830.23905265266061484No Hit
GACTACTACTGTTACTCAACAGACAGTAGTGGTAATCATTGGGTGTTCGG15900.22584296953676625No Hit
GTTTATTACTGTGCGAGAGGAGTTACATACAGTGATGCTGCGGTGACAAC15670.22257605865667462No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC15540.22072954381140547No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGCTCAGCCTGGTCCCTCCTCCGAACAC15420.2190250685696186No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG15220.21618427649997374No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA14930.21206512799898866No Hit
GGGTGTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG14760.20965045473979055No Hit
CAGTAGTAGTCAGCTTCATCTTCCATCTGGGCCCCACTGATGGTCAAGGT14600.20737782108407465No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG14460.20538926663532325No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT13550.19246366271843918No Hit
CCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCC13140.1866400389756672No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA13080.18578780135477374No Hit
CCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAAATACATTGGCAAAA12930.1836572073025401No Hit
GTAGTAGTCAGCTTCATCTTCCATCTGGGCCCCACTGATGGTCAAGGTGG12830.18223681126771765No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12760.18124253404334195No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC12710.18053233602593075No Hit
GAGCTGACACAGGCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAG12470.1771233855423569No Hit
CCCCTGTGTTGGTCATGTACGAGGACAGCAAACGACCCTCCGGGATCCCT12220.17357239545530084No Hit
ACACAGGGGCCAGGCCTGACTTCTGCTGATACCAATAAGCATATTTTTTT12000.1704475241786915No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC11990.17030548457520925No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA11990.17030548457520925No Hit
GATCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTACGAACATTC11590.16462390043591954No Hit
AGTCAGGCCTGGCCCCTGTGTTGGTCATGTACGAGGACAGCAAACGACCC11440.1624933063836859No Hit
CAGTAGTGGTAATCATTGGGTGTTCGGAGGAGGGACCAGGCTGAGCGTCC11280.16022067272797003No Hit
GACCAACACAGGGGCCAGGCCTGACTTCTGCTGATACCAATAAGCATATT11250.15979455391752329No Hit
GTAGTGGTAATCATTGGGTGTTCGGAGGAGGGACCAGGCTGAGCGTCCTA11210.15922639550359433No Hit
GCCTGGTCCCTCCTCCGAACACCCAATGATTACCACTACTGTCTGTTGAGTAACAGTAGTAGTCAGCTTCATCTT11130.15809007867573638No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA11040.15681172224439618No Hit
GGATCACCTGCTCTGGAAATACATTGGCAAAAAAATATGCTTATTGGTATCAGCAGAAGTCAGGCCTGGCCC10960.15567540541653824No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT10740.1525505341399289No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA10650.1512721777085887No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGATCTAGT10620.15084605889814198No Hit
TATTTATTGAGGGTGTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG10600.1505619796911775No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG10580.150277900484213No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC10550.14985178167376628No Hit
CTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCCTCTGA10500.14914158365635508No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC10270.14587467277626348No Hit
GTATCAACGCAGAGTACGGGGTGGGCTCAGGAGGCAGAGCTCTGGGAATCTCACCATGGCCTGGACCCCTCTC10230.14530651436233452No Hit
CCCCTGGGATCCTGCAGATCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTACGAACATTCTGTAGGGGC10140.14402815793099433No Hit
GGACCAGGCTGAGCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG10030.14246572229268964No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC9980.14175552427527843No Hit
CTCTGAGGCCTCCTCTGAGCTGACACAGGCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTG9830.13962493022304479No Hit
GGCCTGGACCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCCTCTGAGCTGACACAGG9580.1360739401359887No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA9570.13593190053250648No Hit
CCACTACTGTCTGTTGAGTAACAGTAGTAGTCAGCTTCATCTTCCATCTG9500.13493762330813078No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC9370.13309110846286162No Hit
GTCCCTGAGGTGGAGCCAGAGAATCTCTCAGGGATCCCGGAGGGTCGTTT9270.13167071242803918No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGCTCAGCCTGGTCCCTCCTCCGAACACCCAATGATTAC9250.13138663322107472No Hit
GGCCTGGCCCCTGTGTTGGTCATGTACGAGGACAGCAAACGACCCTCCGG9040.1284038015479476No Hit
CCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCTCTGG9040.1284038015479476No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG8830.1254209698748205No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG8600.1221540589947289No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTACTCCCTTCATGCGTGACC8490.12059162335642425No Hit
GGAAATACATTGGCAAAAAAATATGCTTATTGGTATCAGCAGAAGTCAGG8440.11988142533901301No Hit
CCTACGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTACTCCCTTCA8430.11973938573553078No Hit
GGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGCTCAGCCTGGTCCCTCCTCCGAACACC8370.11888714811463731No Hit
GAGGAGGGACCAGGCTGAGCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCG8360.11874510851115508No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG8210.11661451445892144No Hit
GTATGTAACTCCTCTCGCACAGTAATAAACAGCCGTGTCCTCCGCTCTCA8190.11633043525195695No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT8120.11533615802758125No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC8110.11519411842409902No Hit
GCAAACGACCCTCCGGGATCCCTGAGAGATTCTCTGGCTCCACCTCAGGG8060.11448392040668778No Hit
GTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGAC7990.1134896431823121No Hit
GTACGAGGACAGCAAACGACCCTCCGGGATCCCTGAGAGATTCTCTGGCT7900.1122112867509719No Hit
GCTCTGGGAATCTCACCATGGCCTGGACCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCT7850.1115010887335607No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC7790.11064885111266723No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTACTCCCTTCATGCGTGACCTGG7750.11008069269873827No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA7690.1092284550778448No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC7520.10681378181864667No Hit
GCTCAGGAGGCAGAGCTCTGGGAATCTCACCATGGCCTGGACCCCTCTCC7490.10638766300819995No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC7440.10567746499078874No Hit
GGCCAGGCCTGACTTCTGCTGATACCAATAAGCATATTTTTTTGCCAATGTATTTCCAGAGCAGGTGATCCTGGC7410.10525134618034201No Hit
GGCTGAGCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAG7410.10525134618034201No Hit
CTCCTGTGCAGCCTCTGGATTCATGTTCACTAGCCATAGTATGAGTTGGATCCGCCAGGCTCCAGGGAAGGGG7390.10496726697337752No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG7380.10482522736989527No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGATCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAAC7380.10482522736989527No Hit
GGCCTGACTTCTGCTGATACCAATAAGCATATTTTTTTGCCAATGTATTT7330.10411502935248405No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC7300.10368891054203733No Hit
CCCAATGATTACCACTACTGTCTGTTGAGTAACAGTAGTAGTCAGCTTCA7280.10340483133507285No Hit
GTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTT7240.10283667292114387No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC7210.10241055411069715No Hit
GTCCCTCCTCCGAACACCCAATGATTACCACTACTGTCTGTTGAGTAACAGTAGTAGTCAGCTTCATCTTCCAT7190.10212647490373265No Hit
GATCAGCACTGAGCACCGAGGATTCACCATGGACCTGGGGCTCCGCTGGG7150.1015583164898037No Hit
GACACAGGCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCA7140.10141627688632145No Hit
GACCATCAGTGGGGCCCAGATGGAAGATGAAGCTGACTACTACTGTTACT7090.10070607886891023No Hit
GGCCTGGACCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTG7080.10056403926542799No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGTA7080.10056403926542799No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)