FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004691759

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691759
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1417614
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC48730.34374660521129163No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT41880.29542597632359724No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC35330.24922157935799166No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA32540.2295406224825658No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC31830.22453220693362227No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC31000.21867729861584323No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC31000.21867729861584323No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG30850.21761918265479885No Hit
GAGTTACAGTCGCCCATACAGTTTTGGCCAGGGGACCAAACTGGAGATCAAAGGAACTGTGGCTGCACCAT30360.21416267051538712No Hit
CTCCATCAACAGTCTGCAACTTGAAGATTTTGCAACTTACTTCTGTCAAC29790.2101418298634184No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT29160.2056977428270319No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG26920.1898965444754355No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT26380.18608732701567565No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC24760.1746596746363961No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24010.16936909483117407No Hit
GTTTTGGCCAGGGGACCAAACTGGAGATCAAAGGAACTGTGGCTGCACCA23910.1686636841904778No Hit
GTTGATGGAGAGACTGAAGTCAGTCCCAGATCCACTGCCACTGAACCTTG23080.16280877587269876No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22770.16062200288654033No Hit
CCATTGTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCTCTTGCCG21880.15434384818434355No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT21850.15413222499213466No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21360.15067571285272294No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT20960.14785407028993788No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20860.14714865964924162No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG20040.14136429239553222No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19760.13938914260158267No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG19680.13882481408902564No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC18480.13035988640067042No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG18390.12972501682404378No Hit
GTAGGAGACAGAGTCACCATCTCTTGCCGGGCGAGTCAGAGCGTTGTCACCTATTTAAATTGGTATCAGCAGAAA18200.12838473660672087No Hit
GGCCAAAACTGTATGGGCGACTGTAACTCTGTTGACAGAAGTAAGTTGCA18150.12803203128637272No Hit
CTGTAACTCTGTTGACAGAAGTAAGTTGCAAAATCTTCAAGTTGCAGACTGTTGATGGAGAGACTGAAGTCAGTC18110.12774986703009422No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17990.1269033742612587No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17410.12281199254522035No Hit
CCAGCATACTGCGCGTGGCAGCCGAGGACTGGAAGAAGGGGGACACCTTCTCCTGCATGGTGGGCCACGAGGCC16620.11723924848371983No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16420.11582842720232729No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT16320.11512301656163101No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16230.11448814698500438No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15980.11272462038326371No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG15830.11166650442221931No Hit
GGTCACAGGAGCTGCCCCGCGAGAAGTACCTGACTTGGGCATCCCGGCAG15590.10997351888454826No Hit
GTGCAGCCACAGTTCCTTTGATCTCCAGTTTGGTCCCCTGGCCAAAACTG15590.10997351888454826No Hit
ATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGCCCACCATGCA15440.10891540292350387No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG15230.10743404057804169No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCCTTTGATCTCCAGTTTGGTCCCCTGGCCAAAACTGTATGGGCGA15160.10694025312955431No Hit
CTCCTAATCTACGCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACTGA15020.10595267823257953No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC14840.10468293907932626No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14820.10454185695118699No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14750.10404806950269961No Hit
GATTAGGAGTTGAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAAT14570.10277833034944633No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA14380.10143805013212341No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14320.10101480374770566No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)