FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004691760

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691760
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1417614
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40360.28470373458501397No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC40300.2842804882005962No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC33460.23603040037697143No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC33370.23539553080034478No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA31180.21994703776909652No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29590.20873100858202587No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC29440.20767289262098146No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29390.2073201873006333No Hit
CTCCATCAACAGTCTGCAACTTGAAGATTTTGCAACTTACTTCTGTCAAC29240.2062620713395889No Hit
GAGTTACAGTCGCCCATACAGTTTTGGCCAGGGGACCAAACTGGAGATCAAAGGAACTGTGGCTGCACCAT28440.20061878621401877No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT26720.18848572319404294No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT26720.18848572319404294No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG25060.1767759065584849No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24340.17169694994547177No Hit
GTTTTGGCCAGGGGACCAAACTGGAGATCAAAGGAACTGTGGCTGCACCA23810.16795827354978154No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22820.16097470820688847No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT22540.15899955841293892No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC21870.15427330712027393No Hit
GTTGATGGAGAGACTGAAGTCAGTCCCAGATCCACTGCCACTGAACCTTG21740.15335627328736878No Hit
CCATTGTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCTCTTGCCG21460.15138112349341923No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20890.1473602828414505No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20780.1465843311366846No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20590.14524405091936168No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT19450.13720236961542423No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT18990.13395748066822138No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG18670.13170016661799333No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18090.12760878490195499No Hit
GTAGGAGACAGAGTCACCATCTCTTGCCGGGCGAGTCAGAGCGTTGTCACCTATTTAAATTGGTATCAGCAGAAA17870.1260568814924232No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17420.12288253360928998No Hit
CTCCTAATCTACGCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACTGA17260.12175387658417594No Hit
GGCCAAAACTGTATGGGCGACTGTAACTCTGTTGACAGAAGTAAGTTGCA16900.11921439827766939No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16730.11801520018848571No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA16730.11801520018848571No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC16530.11660437890709319No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16200.11427652379279549No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG16110.11364165421616886No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG16080.11343003102395999No Hit
GGTCACAGGAGCTGCCCCGCGAGAAGTACCTGACTTGGGCATCCCGGCAG16070.11335948995989036No Hit
CTGTAACTCTGTTGACAGAAGTAAGTTGCAAAATCTTCAAGTTGCAGACTGTTGATGGAGAGACTGAAGTCAGTC15710.11082001165338379No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15510.10940919037199125No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15350.10828053334687722No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC15020.10595267823257953No Hit
CCAGCATACTGCGCGTGGCAGCCGAGGACTGGAAGAAGGGGGACACCTTCTCCTGCATGGTGGGCCACGAGGCC14870.10489456227153512No Hit
GTGCAGCCACAGTTCCTTTGATCTCCAGTTTGGTCCCCTGGCCAAAACTG14790.10433023375897811No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCCTTTGATCTCCAGTTTGGTCCCCTGGCCAAAACTGTATGGGCGA14570.10277833034944633No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14360.10129696800398416No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14350.10122642693991453No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)