FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691762

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691762
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences818277
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATACTACTCCTAAGACGTTCGGCCGAGGGACCAAGGTGGAAATCCAACGAACTGTGGCTGCACCAT40090.4899318934786142No Hit
GTTTTATACAGCTCCAACAATAAGAACTACTTAGCTTGGTACCAGCAAAA36360.4443483074802298No Hit
GTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC33340.40744148986223494No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32130.39265432121396543No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT29660.3624689438906385No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25460.3111415816404469No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22690.277289964156392No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22650.27680113213496166No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19190.2345171622812324No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19040.2326840422008684No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA18340.22412948182583647No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17550.2144750494025862No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17130.20934231317756702No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17100.2089756891614942No Hit
CTCCTAAGTTGCTCATTTACTGGGCATCTACCCGGGGATCCGGGGTCCCT16610.2029874968989719No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16350.19981008875967432No Hit
ACTTAGGAGGCTGTCCTGATTTTTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGCTGTATAAAACACT15900.19431072851858233No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14840.18135667995067684No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14230.17390199162386333No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14210.17365757561314812No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14040.17158003952206893No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13840.16913587941491695No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCG13660.16693613531848017No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13590.16608067928097697No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC13390.163636519173825No Hit
GTTTATTACTGTCAGCAATATTATACTACTCCTAAGACGTTCGGCCGAGGGACCAAGGTGGAAATCCAACGAA13310.1626588551309642No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT12790.15630403885236904No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12640.15447091877200508No Hit
CTCCTAAGACGTTCGGCCGAGGGACCAAGGTGGAAATCCAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC12170.14872714252019792No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12110.14799389448805234No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11940.14591635839697317No Hit
GTGCAGCCACAGTTCGTTGGATTTCCACCTTGGTCCCTCGGCCGAACGTC11600.1417612862148148No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA11520.140783622171954No Hit
GTCCCTGACCGATTCAGCGGCAGCGGGTCTGGGACAGATTTCACTCTCAC11160.13638413397908045No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11120.13589530195765004No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10140.12391891743260536No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10030.12257462937367175No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATA10000.12220800535759896No Hit
GTCCCAGACCCGCTGCCGCTGAATCGGTCAGGGACCCCGGATCCCCGGGT9940.12147475732545336No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9840.12025267727187737No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCGCTGAATCGGTCAGGGACCCCG9680.1182973491861558No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT9550.116708645116507No Hit
GTGCTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC9550.116708645116507No Hit
TCTTAGGAGTAGTATAATATTGCTGACAGTAATAAACTGCCACATCTTCA9550.116708645116507No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATACTACT9250.11304240495577902No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC9230.11279798894506385No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9230.11279798894506385No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9000.10998720482183906No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC8920.10900954077897827No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT8680.10607654865039588No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT8640.1055877166289655No Hit
GTCAGGGACCCCGGATCCCCGGGTAGATGCCCAGTAAATGAGCAACTTAGGAGGCTGTCCTGATTTTTGCTGGTA8520.10412122056467431No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA8470.10351018053788631No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT8440.1031435565218135No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC8390.10253251649502551No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC8290.10131043644144952No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAACT150.002547318667.6494731
GAATTAG200.00819749750.3552671
AATGGTA2000.044.8486170
GGTATCA12150.044.483941
GTGGTAT3200.043.011791
GTATCAA30850.038.4125561
TGGTATC3700.037.1857382
GTTTACG400.002399863235.0566147
TATCAAC35100.033.6534652
GTACTGG809.014366E-833.570181
TTACGAC450.004149531531.3497249
TTTACGA450.004209931531.25729448
ATCAACG37950.031.2126483
AACGCAG38350.031.058296
TCAACGC38150.031.045224
CAACGCA38800.030.5251335
CGCAGAG40700.029.2658
ACGCAGA41000.029.0508657
AGAGTAC41550.028.66982311
CAGAGTA41700.028.56494710
Produced by FastQC (version 0.11.5)