FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691763

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691763
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences825868
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT21660.2622695152251934No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21060.25500443170094983No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15260.18477529096659515No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15060.18235359645851396No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14850.1798108172250287No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14500.1755728518358866No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCAGAAGATT14300.17315115732780542No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13890.16818668358623898No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA13480.1632222098446725No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12630.15293000818532743No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12540.15184024565669088No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12480.15111373730426653No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12120.1467546871897204No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11760.14239563707517422No Hit
GTTTATTACTGTCAGCAGCGTGTCAACTGGCCCCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA11350.13743116333360778No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTT11260.13634140080497126No Hit
GTCTCCAGCCACCCTGGTTCTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCCG11060.13391970629689007No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT10300.12471726716618153No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCAGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTGTCAACTGG10080.12205340320729222No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9970.12072147122784754No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9920.12011604760082725No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9670.11708892946572576No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG9640.11672567528951358No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9540.11551482803547299No Hit
GCCTAGAGCCAGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTGTCAACTGGCCCCTCACTTTCGGCGGAGGGA9080.10994493066688624No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9050.10958167649067405No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC9010.1090973375890578No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC8950.10837082923663345No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCCGCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCT8930.10812865978582535No Hit
GTAATAAACTGCAAAATCTTCTGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA8560.10364852494587513No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8500.10292201659345077No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12050.045.080751
CGGGATA350.001456751738.80869317
GTATCAA30750.038.423091
CCCACGA1500.036.2214519
TATCAAC35950.033.0522542
TCGTATG1600.032.44437440
ATCAACG36550.032.3239023
TCAACGC37100.031.8447044
CGAGACA1850.031.21941223
CAACGCA37900.031.172525
AACGCAG38250.031.0647956
GGCAATC1550.030.93177433
CGTACCA553.5783288E-430.86307514
CACGAGA1800.030.18819621
GCCCACG1800.030.1845418
CGCAGAG40150.029.5929378
ACGCAGA40750.029.3238287
AGAGTAC40700.029.27644311
ATCTCCG1650.028.80379310
GAGTTTA2950.028.7724061
Produced by FastQC (version 0.11.5)