FastQCFastQC Report
Wed 8 May 2024
EGAF00004691764

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691764
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences825868
Total Bases60.9 Mbp
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT22690.2747412419418115No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20840.2523405677420605No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17370.21032416802685172No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16300.19736810240861738No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16020.19397773009730368No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15370.1861072229460398No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCAGAAGATT14860.17993190195043277No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT14070.17036620864351204No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13740.16637041270517808No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13660.1654017349019456No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTC13390.162132447316036No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13380.16201136259063192No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA13240.1603161764349751No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13110.15874207500472232No Hit
GTTTATTACTGTCAGCAGCGTGTCAACTGGCCCCTCACTTTCGGCGGAGG12800.15498844851719645No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA12090.1463914330135082No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGC11990.1451805857594676No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTC11830.14324323015300267No Hit
GTCTCCAGCCACCCTGGTTCTGTCTCCAGGGGAAAGAGCCACCCTCTCCT11650.14106370509572957No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTT11430.13839984113684026No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG11180.13537272300173878No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCAGAAGATTTTGCAGTTTA11010.13331428266986975No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC10870.1316190965142129No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10610.12847089365370737No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10200.12350641991214091No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10090.12217448793269627No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCCGCTACTTAGCCTGGTACCAAC9990.12096364067865567No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG9840.11914736979759476No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9470.11466723495764455No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAA9180.11115577792092683No Hit
GCCTAGAGCCAGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTGTCAAC9180.11115577792092683No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9170.11103469319552277No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG9060.10970276121607811No Hit
GTAATAAACTGCAAAATCTTCTGGCTCTAGGCTGCTGATGGTGAGAGTGA8950.10837082923663345No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC8750.10594913472855227No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCT8370.10134791516319798No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)