FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691766

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691766
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1024588
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG47250.4611609739719771No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG39840.38883922122843523No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG34500.3367207111541419No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC33080.32286148188344976No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG27070.2642037579983369No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG24990.24390291512295675No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC24230.23648529945695246No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC22410.21872206194099483No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT21320.20808363947264655No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT21150.20642443596840873No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT21110.20603403514388224No Hit
GTACAACGTGTCCCTGGTCATGTCCGACACAGCTGGCACCTGCTACTGAC20780.20281322834153825No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC19200.18739239577273986No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC18410.17968197948834067No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT18120.17685157351052325No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG17980.17548517062468036No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG17870.17441156835723237No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT17120.16709155289735972No Hit
ATGCAACATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGG17090.16679875227896482No Hit
CCCCGAGCCTGTGGGCAGGCCAGCAGGGTCAGTAGCAGGTGCCAGCTGTG16990.16582275021764847No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT16880.1647491479502005No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC16860.1645539475379372No Hit
CACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGGGTCA16660.16260194341530448No Hit
CGGTTAGTTTGCATGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGG16050.15664833084127475No Hit
ACCTACACCTGCGTGGTGGCCCATGAGGCCCTGCCCAACAGGGTCACCGA15300.1493283153814021No Hit
GGCCAGCAGGGTCAGTAGCAGGTGCCAGCTGTGTCGGACATGACCAGGGA15280.14913311496913884No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG15170.14805951270169082No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15140.14776671208329592No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC15010.14649790940358465No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG14760.14405790425029377No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC14660.14308190218897743No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCCAC14250.13908029373758038No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGT14070.13732349002721092No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG13660.1333218815758139No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG13600.13273628033902407No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13280.12961307374281175No Hit
GGTCAGTAGCAGGTGCCAGCTGTGTCGGACATGACCAGGGACACGTTGTA12920.12609946632207286No Hit
GAATATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC12780.12473306343622997No Hit
GGCCGGTACTTCGCCCACAGCATCCTGACCGTGTCCGAAGAGGAATGGAA12610.12307385993199217No Hit
GTTCATAGGTTCTCAACCCTCACCCCCCACCACGGGAGACTAGAGCTGCAGGATCCCAGGGGAGGGGTCTCTCCT12320.12024345395417475No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC12310.12014585374804311No Hit
GACCTAGGACGGTCAACTTGGTCCCCCCGCCGAATATCAAATTGTCGTTGCCTGCATATGCGCTGCAGTAATAAT12290.11995065333577984No Hit
GTGTCGGACATGACCAGGGACACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACC12230.11936505209899004No Hit
GCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCCCCGCCGAATATCAAATTGTCGTTGCCTGCATATGCGCTG11990.11702264715183078No Hit
GTTAGTTTGCATGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGGGTCAGTAGCAGGTG11910.1162418455027777No Hit
CACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGGGT11830.11546104385372462No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC11830.11546104385372462No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA11800.1151682432353297No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA11770.11487544261693482No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA11660.11380184034948682No Hit
GGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTG11440.11165463581459086No Hit
GACCTACACCTGCGTGGTGGCCCATGAGGCCCTGCCCAACAGGGTCACCG11430.11155703560845921No Hit
ACCCTGTACAACGTGTCCCTGGTCATGTCCGACACAGCTGGCACCTGCTACTGACCCTGCTGGCCTGCCCACAGG11320.11048343334101121No Hit
CATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCCACCA11050.10784822777545706No Hit
GGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACC10980.1071650263325356No Hit
GCCCAACAGGGTCACCGAGAGGACCGTGGACAAGTCCACCGGTAAACCCA10970.10706742612640399No Hit
CCGAGAGGACCGTGGACAAGTCCACCGGTAAACCCACCCTGTACAACGTG10950.10687222571414072No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC10890.10628662447735089No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG10850.10589622365282435No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAAC10830.10570102324056108No Hit
CGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCAT10740.10482262138537636No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA10600.10345621849953347No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT10430.10179701499529567No Hit
CCTGTGGGCAGGCCAGCAGGGTCAGTAGCAGGTGCCAGCTGTGTCGGACA10430.10179701499529567No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC10370.10121141375850586No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATACG150.002406141768.6305238
GGTATCA17250.044.5730441
GTGGTAT4550.043.5378071
TGGTATC4550.043.510172
GTATCAA47350.040.8440171
ACACGAG400.001807372637.1422564
TATCAAC55900.034.6949422
TAACCGG400.002554383834.6143341
ATCAACG57700.033.6608963
TCAACGC57900.033.6604964
CAACGCA59250.033.0083855
AACGCAG60000.032.5973746
ACGCAGA62950.031.0697787
CGCAGAG63300.030.791998
AGAGTAC64350.030.0810211
GCAGAGT64900.029.928029
CAGAGTA64800.029.91951610
ACGGAAC656.0750335E-428.1960161
CGTAAGA500.007313957427.91790244
CGAACTA500.008834220526.8579924
Produced by FastQC (version 0.11.5)