FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691770

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691770
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences288026
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21180.7353502808774208No Hit
GTATCAACGCAGAGTACGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCC18420.6395255983834793No Hit
ATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCT14620.5075930645150091No Hit
CTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCG12570.4364189344017554No Hit
GTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCA12210.42392006277211086No Hit
GGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCA10510.3648976134099005No Hit
TCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACCCGAT8680.30136168262587404No Hit
GCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGAC8600.29858415559706414No Hit
GTATCAACGCAGAGTACGGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCA8540.2965010103254567No Hit
GAGTACGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGG8170.28365494781721096No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTC7780.2701145035517627No Hit
ATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC7750.26907293091595896No Hit
TCAATATGGTGACCTCCCGGGAGCGGGGGACCACCAGGTTGCCTAAGGAG7670.26629540388714906No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAG7450.25865720455792185No Hit
TGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCG7100.24650552380687854No Hit
GGTATCAACGCAGAGTACGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCC6940.24095046974925874No Hit
GTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGC6750.23435384305583526No Hit
CCATATTGATGCCGAACTTAGTGCGGACACCCGATCGGCATAGCGCACTA6690.2322706977842278No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6650.23088193426982284No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6520.22636845284800677No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC6150.213522390339761No Hit
ACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACCCGATCGGCATAGCG6000.20831452716074242No Hit
GCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAAC5730.19894037343850904No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT5680.19720441904550284No Hit
ATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCACCAGGTTGC5680.19720441904550284No Hit
GTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCAC5480.1902606014734781No Hit
GTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCT5310.18435835653725705No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4990.17324824842201747No Hit
GTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCC4950.17185948490761252No Hit
GGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGAC4740.16456847645698652No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACC4740.16456847645698652No Hit
CCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTC4660.16179094942817662No Hit
GCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCG4580.15901342239936672No Hit
GGCTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGC4470.15519432273475312No Hit
GTACGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGT4320.14998645955573456No Hit
TATCAACGCAGAGTACGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCA4280.1485976960413296No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT4140.1437370237409123No Hit
TCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAA4080.14165387846930486No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3910.1357516335330838No Hit
CCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACCCGATCGGCATAGCGCACTACAGCCCAGAACTC3790.13158534298986896No Hit
GTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGT3780.13123815211126774No Hit
CCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGC3730.12950219771826155No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3730.12950219771826155No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3720.1291550068396603No Hit
ATATGGTGACCTCCCGGGAGCGGGGGACCACCAGGTTGCCTAAGGAGGGG3720.1291550068396603No Hit
ATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCAC3690.1281134342038566No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3630.12603028893224918No Hit
GGGGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCT3610.1253359071750467No Hit
CCTCCCGGGAGCGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGC3580.12429433453924298No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3550.12325276190343927No Hit
GTATCAACGCAGAGTACGGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCA3350.11630894433141453No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3210.1114482720309972No Hit
CTACAGCCCAGAACTCCTGGACTCAAGCGATCCTCCAGCCTCAGCCTCCCGAGTAGCTGGGACTACAGGCACGCG3200.11110108115239598No Hit
GGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCG3200.11110108115239598No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3190.11075389027379473No Hit
GAGTACGGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG3130.10867074500218729No Hit
GGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAAC3100.1076291723663836No Hit
GTATCAACGCAGAGTACGGGTCTGGTGCAACGTGGGGGCTGGAGGGTGGG3100.1076291723663836No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3100.1076291723663836No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3090.10728198148778234No Hit
GATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTC3080.10693479060918112No Hit
GGTATCAACGCAGAGTACGGGGTCTCGCTATGTTGCCCAGGCTGGAGTGC3050.10589321797337739No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG3040.10554602709477616No Hit
ACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGT2930.10172692743016255No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA2920.10137973655156131No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCGA150.003677544661.6524772
CTAGGGT200.00437096859.01696454
GAAATTA301.9647523E-458.2467465
TCCCTAG200.004734972557.8368851
GATTTGA400.00112929840.857959
ACAACAC350.001407217939.07276537
CAGGTAT409.6663076E-538.552891
TTAGGGT350.002340028535.229992
CTCTAGA350.002340028535.229992
GGGTACC350.00234402235.217755
CTCTGTC501.82542E-434.60210838
GACTCTG400.002843665733.85591536
GTTGACA950.003075561333.30220470
ATTGCGC450.004282636631.14007239
GGTACCG400.00450890930.8155296
TACAGAT500.00500864830.15809448
TTTTAAG550.005145509729.99112360
ATTGTCA500.005551140829.53058645
CAGTGTT550.006259911728.81237255
TGTTGAC905.36534E-428.7845669
Produced by FastQC (version 0.11.5)