FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691771

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691771
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1186788
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC64600.5443263666299288No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT45930.3870109910110314No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG44690.37656262112525574No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG40220.3388979329079836No Hit
GCCTTGGGCTGACCTAGGACGGTCAACTTCGTCCCCCCGCCGAAAATCACGTGAGTGCTGGAGTCGCTATATGAG39330.3313986996835155No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC33030.27831423977997755No Hit
GACCTAGGACGGTCAACTTCGTCCCCCCGCCGAAAATCACGTGAGTGCTG32600.2746910147389424No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT32010.26971961293845237No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT31610.26634917103981504No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT31600.2662649099923491No Hit
TTTCTGGACTCCAGCCTGAGGACGAGGCTGATTATCACTGCAGCTCATAT30630.25809158838815355No Hit
GATTATCACTGCAGCTCATATAGCGACTCCAGCACTCACGTGATTTTCGG30530.2572489779134942No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC30410.25623784534390304No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG27480.2315493584363846No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC27060.2280103944428154No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT26940.2269992618732242No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTCGTCCCCC26730.2252297798764396No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC25790.21730924141464186No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG24540.2067766104814002No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC23770.20028850982652335No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA23750.20011998773159148No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG23730.1999514656366596No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG23150.19506432488363548No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA22650.19085127251033884No Hit
GTCCAGGAGACCCAGACACGGAGGCAGGCTGAGTCAGGGCAGACTGGGCC22450.18916605156102018No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG22190.1869752643269059No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC21750.18326777823840484No Hit
GGATTATAGTTGGTGGCCCCACTGCTATAGATTCGACCAATCCACTCCAGTCCCTTCCCGGCGGGCTGCCGGATC21710.1829307340485411No Hit
GGTCAGTAGTCGACCCTCAGGAGTTTCTGATCGCTTCTCTGGCTCCAAGT21350.1798973363397675No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT21130.17804359329551697No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20920.17627411129873236No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT20460.17239810311529946No Hit
GTAGTCGACCCTCAGGAGTTTCTGATCGCTTCTCTGGCTCCAAGTCTGGC19330.162876604751649No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA19030.160348773327671No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA18940.1595904239004776No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC18310.1542819779101238No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG18080.15234397381840734No Hit
GAATAAATGTCACTGATGGTTCCACTGCAGGCGATGGTGATCGACTGTCCAGGAGACCCAGACACGGAGGCAGG17990.15158562439121392No Hit
CTATAATCCCTCCCTCAAGAGGCGAGTCATCATATCACTGGACACGTCCA17390.14652996154325795No Hit
CTCCAGCACTCACGTGATTTTCGGCGGGGGGACGAAGTTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGT17290.14568735106859862No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT17240.14526604583126893No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT16570.13962055565105141No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA16530.13928351146118767No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT16080.1354917643252207No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC15820.13330097709110642No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC15820.13330097709110642No Hit
CATATAGCGACTCCAGCACTCACGTGATTTTCGGCGGGGGGACGAAGTTGACCGTCCTAGGTCAGCCCAAGGCTG15740.13262688871137895No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG15520.13077314566712842No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAAC15460.13026757938233283No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC15430.130014796239935No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC15320.12908792471780975No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCGCCTG15250.1284980973855482No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA15210.12816105319568447No Hit
ACCTAGGACGGTCAACTTCGTCCCCCCGCCGAAAATCACGTGAGTGCTGG15120.12740270376849108No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC15090.1271499206260933No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC15050.12681287643622954No Hit
CACCCAGGCAGAGCCCCCAAACTCATCATTTATGAGGTCAGTAGTCGACCCTCAGGAGTTTCTGATCGCTTCT14950.12597026596157024No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG14780.12453782815464935No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCCAC14550.12259982406293288No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT14550.12259982406293288No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC14510.12226277987306916No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14300.12049329787628457No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC14270.12024051473388676No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG13680.11526911293339669No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG13570.11434224141127142No Hit
CCTCAGGAGTTTCTGATCGCTTCTCTGGCTCCAAGTCTGGCTACACGGCCTCCCTGACCATTTCTGGACTCCAGC13490.11366815303154396No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC13470.1134996309366121No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC13350.11248849836702089No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA13270.11181440998729343No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA13260.1117301489398275No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT13080.1102134500854407No Hit
CGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCAT12830.10810692389879237No Hit
GTGATCGACTGTCCAGGAGACCCAGACACGGAGGCAGGCTGAGTCAGGGCAGACTGGGCCCAGGACCCTGTGCC12710.10709579132920116No Hit
GTTCCACTGCAGGCGATGGTGATCGACTGTCCAGGAGACCCAGACACGGA12700.10701153028173523No Hit
ATCCACTCCAGTCCCTTCCCGGCGGGCTGCCGGATCCAGCTCCAGAAGTA12550.10574761456974624No Hit
TTTATGAGGTCAGTAGTCGACCCTCAGGAGTTTCTGATCGCTTCTCTGGCTCCAAGTCTGGCTACACGGCCTCC12530.10557909247481438No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT12480.1051577872374847No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC12250.10321978314576823No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG12220.10296700000337045No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA12220.10296700000337045No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGGTA100.0076498776141.3603470
TACGTAT652.0008883E-1186.9909770
AGGGGGG3150.078.53351670
GTATAAA252.9901843E-453.5214541
GTACGTA851.0617441E-842.7955169
CTTATAC5100.041.9776081
TATACAC5150.041.550793
GGTACGT801.7515049E-840.43703568
TTATACA5300.040.3765262
GGTATCA5850.040.0267221
AATGGTA6250.039.5808970
CGTATGC5000.039.2816841
GTATCAA15400.039.098461
CTTGAAA5450.038.77537557
AAGTCGG350.001555760938.29418627
TATTAGA350.001571968438.2134972
ATTAGAG350.001572293938.2118873
CTAGCTC451.1100703E-437.6695135
TATCTCG4900.037.44038836
TCGTATG5150.037.35006740
Produced by FastQC (version 0.11.5)