FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004691772

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691772
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1186788
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC59920.5048921964158721No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG58670.49435956548263044No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG44410.3742033117962096No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT43880.3697374762805151No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG40110.3379710613858583No Hit
GCCTTGGGCTGACCTAGGACGGTCAACTTCGTCCCCCCGCCGAAAATCACGTGAGTGCTGGAGTCGCTATATGAG38260.32238276760466067No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC31750.2675288257043381No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC31220.26306299018864365No Hit
TTTCTGGACTCCAGCCTGAGGACGAGGCTGATTATCACTGCAGCTCATAT30730.25893419886281294No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG28650.24140790098989878No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT28500.24014398527790978No Hit
GATTATCACTGCAGCTCATATAGCGACTCCAGCACTCACGTGATTTTCGG28430.23955415794564824No Hit
GACCTAGGACGGTCAACTTCGTCCCCCCGCCGAAAATCACGTGAGTGCTG28400.23930137480325048No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT27690.23331884043316917No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC27520.23188640262624835No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG27470.23146509738891866No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT26570.22388160311698468No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTCGTCCCCC25880.21806759084183527No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC23990.20214225287077386No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC23550.19843476678227281No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG22680.1911040556527366No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA22380.18857622422875864No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22150.18663822013704215No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT21560.18166681833655213No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC21500.1811612520517565No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG21490.18107699100429056No Hit
GTCCAGGAGACCCAGACACGGAGGCAGGCTGAGTCAGGGCAGACTGGGCC20920.17627411129873236No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA20910.17618985025126646No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG20730.17467315139687964No Hit
GGTCAGTAGTCGACCCTCAGGAGTTTCTGATCGCTTCTCTGGCTCCAAGT20680.17425184615954997No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA20210.17029157692865113No Hit
GGATTATAGTTGGTGGCCCCACTGCTATAGATTCGACCAATCCACTCCAGTCCCTTCCCGGCGGGCTGCCGGATC19490.16422478151110392No Hit
GTAGTCGACCCTCAGGAGTTTCTGATCGCTTCTCTGGCTCCAAGTCTGGC18710.15765241980876113No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT18500.15588293781197654No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT17390.14652996154325795No Hit
GAATAAATGTCACTGATGGTTCCACTGCAGGCGATGGTGATCGACTGTCCAGGAGACCCAGACACGGAGGCAGG17120.14425491326167775No Hit
CTCCAGCACTCACGTGATTTTCGGCGGGGGGACGAAGTTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGT17050.1436650859294162No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA17000.14324378069208654No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG16920.14256969231235908No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC16860.14206412602756346No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC16860.14206412602756346No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC16380.13801959574919867No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT15950.13439637070816354No Hit
CTATAATCCCTCCCTCAAGAGGCGAGTCATCATATCACTGGACACGTCCA15630.1317000171892537No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA15370.12950922995513942No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC15270.12866661948048008No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC15250.1284980973855482No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCGCCTG15220.12824531424315042No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT15180.1279082700532867No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC15000.12639157119889988No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG15000.12639157119889988No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC14940.1258860049141043No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC14590.12293686825279662No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAAC14540.12251556301546697No Hit
CATATAGCGACTCCAGCACTCACGTGATTTTCGGCGGGGGGACGAAGTTGACCGTCCTAGGTCAGCCCAAGGCTG14470.12192573568320542No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT14450.12175721358827354No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA14440.12167295254080762No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC14290.12040903682881864No Hit
ACCTAGGACGGTCAACTTCGTCCCCCCGCCGAAAATCACGTGAGTGCTGG14130.11906086006936369No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT14130.11906086006936369No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG13890.11703859493018129No Hit
CACCCAGGCAGAGCCCCCAAACTCATCATTTATGAGGTCAGTAGTCGACCCTCAGGAGTTTCTGATCGCTTCT13830.1165330286453857No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA13770.11602746236059011No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC13570.11434224141127142No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA13470.1134996309366121No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCCAC13400.11290980360435057No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC13320.11223571522462311No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12820.10802266285132643No Hit
GTGATCGACTGTCCAGGAGACCCAGACACGGAGGCAGGCTGAGTCAGGGCAGACTGGGCCCAGGACCCTGTGCC12570.10591613666467811No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTCGTCCCCCCGCCGAAAATCACGTGAGTG12340.10397813257296165No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG12340.10397813257296165No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT12290.10355682733563197No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA12240.10313552209830232No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG12100.10195586743377924No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA12060.10161882324391551No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC12040.10145030114898364No Hit
GTTCCACTGCAGGCGATGGTGATCGACTGTCCAGGAGACCCAGACACGGA11970.10086047381672211No Hit
TTTATGAGGTCAGTAGTCGACCCTCAGGAGTTTCTGATCGCTTCTCTGGCTCCAAGTCTGGCTACACGGCCTCC11940.1006076906743243No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG11930.10052342962685837No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)