FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691774

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691774
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences295815
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG77762.6286699457431166No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6180.20891435525581867No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6170.20857630613728173No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT5410.18288457312847556No Hit
GTATCAACGCAGAGTACGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCC4920.16632016632016633No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT4240.14333282625965554No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4170.14096648242989707No Hit
GTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCA3370.11392255294694319No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3290.11121815999864781No Hit
ATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCT3070.1037810793908355No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2980.10073863732400319No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2960.10006253908692933No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTATAT450.00770914751.11708570
TATCAGC254.2575068E-449.8112682
ACGGGTA1153.6379788E-1232.4856116
CACGAAT652.9862608E-431.81726361
ATCCATA450.00394340331.66982841
ACTACAC400.00430342231.110993
GTTTAGG555.9043296E-428.3258171
TAAGACT555.96307E-428.2779399
ATGACAG1100.00733466327.88204670
ACTGTCT450.007658475527.6495388
GTATCAA38000.027.3045961
CGGGTAT806.123153E-627.24053817
CGAACTA608.1679755E-426.79560529
AAAGCGA609.35969E-426.17808725
AGTCTAC809.831029E-525.75888838
CGGGTGG2300.025.71777317
AGAGTAC41050.025.09008211
GCAGAGT40750.025.037259
CAGAGTA41300.024.92977310
CGCAGAG41950.024.7659448
Produced by FastQC (version 0.11.5)