FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691776

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691776
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1408280
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG53330.37868889709432785No Hit
GACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACGAGGGTGCCAGTGCCCCAGGTCTGACAGTAATAGTCAG48320.34311358536654646No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC47580.3378589485045587No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG47510.3373618882608572No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC47120.3345925526173772No Hit
GGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCAC41140.2921294060840174No Hit
CCTCTGAGGAGCTTCAAGCCAACACGGCCACACTGGTGTGTCTCATAAGT40720.2891470446218082No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG39900.2833243389098759No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA38730.27501633197943587No Hit
GCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACGAG38060.27025875536114974No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG37910.2691936262675036No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACACGGCCACACTGGTGT37730.2679154713551282No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT36730.2608146107308206No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36460.2588973783622575No Hit
GACTATTACTGTCAGACCTGGGGCACTGGCACCCTCGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAAGTCAG36170.2568381287812083No Hit
GCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTCTCT36090.25627005993126367No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT32110.2280086346465192No Hit
GTAATAGTCAGCCTCATCCTCAGACTGGAGACTGGAGATGGTGAGGTAGC31390.22289601499701764No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTC31070.2206237395972392No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACACGG30860.21913255886613459No Hit
CACCAAGGGGGACGGGATCCCTGATCGCTTCTCAGGCTCCAGCTCTGGGG30750.21835146419746074No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC29930.21252875848552844No Hit
CTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCACCTGCACT29780.2114636293918823No Hit
CCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGC28640.20336864828017157No Hit
CTATTACTGTCAGACCTGGGGCACTGGCACCCTCGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAAGTCAGCC28600.20308461385519927No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG28370.20145141591160848No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC28360.20138040730536538No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG28270.20074132984917772No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA28140.19981821796801771No Hit
GCCCCAGAGCTGGAGCCTGAGAAGCGATCAGGGATCCCGTCCCCCTTGGT27430.1947766069247593No Hit
GTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC26900.19101315079387624No Hit
CCTGCACTCTGAGCAGTGGGCACACCAACTACGCCATCGCATGGCATCAG26900.19101315079387624No Hit
CACCTGCACTCTGAGCAGTGGGCACACCAACTACGCCATCGCATGGCATC26570.1886698667878547No Hit
GCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAG26090.18526145368818703No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT26000.18462237623199934No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT25550.18142698895106088No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGG25520.18121396313233165No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC25230.17915471355128243No Hit
CCACTGCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGT25000.17752151560769164No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA23980.17027863777089786No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC23760.16871644843355016No Hit
GCTCTGGGGCTGAGCGCTACCTCACCATCTCCAGTCTCCAGTCTGAGGATGAGGCTGACTATTACTGTCAGAC23260.1651660181213963No Hit
CACCAGTGTGGCCGTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG22870.16239668247791633No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC22190.1575680972533871No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACACGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC22010.15628994234101173No Hit
GTCTGAGGATGAGGCTGACTATTACTGTCAGACCTGGGGCACTGGCACCCTCGTGTTCGGCGGAGGGACCAAG21950.15586389070355325No Hit
GTTCTTGATGAACCTTAACCATGATGGCAGCCACACCAAGGGGGACGGGATCCCTGATCGCTTCTCAGGCTCCAG21770.15458573579117788No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA21380.1518164001476979No Hit
GGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGT21240.15082227966029482No Hit
CTTATGAGACACACCAGTGTGGCCGTGTTGGCTTGAAGCTCCTCAGAGGA21050.14947311614167635No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT20930.14862101286675947No Hit
CAGTAATAGTCAGCCTCATCCTCAGACTGGAGACTGGAGATGGTGAGGTAGCGCTCAGCCCCAGAGCTGGAGC20750.1473428579543841No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC20070.14251427272985484No Hit
CTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGC19870.14109410060499333No Hit
TCCTCAGACTGGAGACTGGAGATGGTGAGGTAGCGCTCAGCCCCAGAGCTGGAGCCTGAGAAGCGATCAGGGATC19380.13761467889908258No Hit
GTGTGGCCGTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA18950.13456130883063028No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT18870.13399323998068566No Hit
GTATCAACGCAGAGTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTC18800.1334961797369841No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA18570.13186298179339337No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT18550.1317209645809072No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTC18520.13150793876217798No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC18440.13093986991223336No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT18150.12888062033118414No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG18030.12802851705626722No Hit
CGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC17400.12355497486295339No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA17380.12341295765046724No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAAC16520.11730621751356264No Hit
CCCTTGGTGTGGCTGCCATCATGGTTAAGGTTCATCAAGAACCGAGGGCC16380.11631209702615958No Hit
TCCTCACCCTCCTCCTCCACTGCACAGGGTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCT16380.11631209702615958No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGC16360.11617007981367342No Hit
GTGGGCACACCAACTACGCCATCGCATGGCATCAGCAGCAGCCAGAGAAG16350.11609907120743033No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG16300.11574402817621497No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTC16300.11574402817621497No Hit
CTCCAGCTCTGGGGCTGAGCGCTACCTCACCATCTCCAGTCTCCAGTCTG16220.11517595932627035No Hit
GTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCA16190.11496293350754111No Hit
GAGTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTG15870.11269065810776266No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG15810.11226460647030422No Hit
GAATATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC15440.10963728803931036No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG15440.10963728803931036No Hit
CTACCTCACCATCTCCAGTCTCCAGTCTGAGGATGAGGCTGACTATTACTGTCAGACCTGGGGCACTGGCACCCT15260.10835913312693499No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCATCCCTCACCCCCCAC15220.10807509870196268No Hit
GCTTGGTCCCTCCGCCGAACACGAGGGTGCCAGTGCCCCAGGTCTGACAGTAATAGTCAGCCTCATCCTCAGA15200.10793308148947653No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA15150.10757803845826114No Hit
GGTCACAGGAGCTGCCCCGCGAGAAGTACCTGACTTGGGCATCCCGGCAG14900.10580282330218421No Hit
GTCTCTCTCCCAGCTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCACCTG14840.10537677166472578No Hit
GGACCAAGCTGACCGTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG14810.10516374584599653No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC14780.10495072002726731No Hit
GGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGT14740.10466668560229499No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG14740.10466668560229499No Hit
GAGTACGGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACAT14560.10338853068991961No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC14490.10289147044621808No Hit
GTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCA14480.10282046183997501No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACACGGCC14200.10083222086516887No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC14150.10047717783395348No Hit
CTCTGAGCAGTGGGCACACCAACTACGCCATCGCATGGCATCAGCAGCAG14130.10033516062146733No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT14120.10026415201522425No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG14120.10026415201522425No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC4950.049.312522
GTGGTAT5100.047.879161
GGTATCA20200.045.499031
GTATCAA60450.042.470151
TGCGTAC405.5552067E-542.365911
GTGCGTA558.351646E-636.97519310
TATCAAC71450.035.7765922
ATCAACG73050.034.8902473
TCAACGC74300.034.26134
AACGCAG76650.033.5216376
CAACGCA76300.033.453275
CGCAGAG81100.031.6822858
TCGTTAA1352.8935656E-530.83331970
CAGAGTA84150.030.53071210
AGAGTAC84200.030.511511
ACGCAGA84350.030.4615697
GCAGAGT84700.030.3356979
TAGGCCG450.005039021430.1311455
ACGCCAA500.0053472829.76722567
AATGGTA4750.029.21051470
Produced by FastQC (version 0.11.5)