FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691777

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691777
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1072886
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT35820.33386585340847025No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31950.29779491949750486No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28650.2670367587982321No Hit
GATCTATGCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAATGGATCTGGGACAGATTTCA26200.24420115464271133No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25810.24056609928734277No Hit
GAGTTACAGTACCCCTCAGGTCACCTTCGGCCAAGGGACACGACTGGAGGTTCAACGAACTGTGGCTGCACCAT24050.22416174691439725No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23640.22034027846388154No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23140.21567995108520382No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG22430.20906228620748152No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21790.20309706716277404No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21720.20244462132975918No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGGATTGGCACCTATTTAAATTGGTATCAACAGAAG21590.20123293621130298No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTGCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC20620.19219190109666826No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC20410.1902345635976236No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19910.1855742362189459No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGGATTGGCAC19830.18482858383835746No Hit
GCATAGATCAGGAGGTTAGGGGCTTTCCCTGGCTTCTGTTGATACCAATT19810.18464217074321038No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG18830.1755079290810021No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18410.17159325408291282No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18390.1714068409877657No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18000.16777178563239709No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17910.16693292670423512No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17600.16404352372945494No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17600.16404352372945494No Hit
GGACACGACTGGAGGTTCAACGAACTGTGGCTGCACCATCTGTCTTCATC17350.1617133600401161No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17250.16078129456438056No Hit
GTAGTAAGTTGCAAAATCTGCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCATTGCCA17040.15882395706533592No Hit
GTACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTGCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT16710.15574814099540865No Hit
ACTTACTACTGTCAACAGAGTTACAGTACCCCTCAGGTCACCTTCGGCCA16320.15211308564004003No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15900.14819841064195077No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT15540.14484297492930284No Hit
GTCGTGTCCCTTGGCCGAAGGTGACCTGAGGGGTACTGTAACTCTGTTGA15260.14223319159724332No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGCAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTACC14970.13953020171761024No Hit
GATCAGGAGGTTAGGGGCTTTCCCTGGCTTCTGTTGATACCAATTTAAAT14850.13841172314672762No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT14560.13570873326709457No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14470.13486987433893255No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14380.13403101541077056No Hit
AGTCAGAGGATTGGCACCTATTTAAATTGGTATCAACAGAAGCCAGGGAAAGCCCCTAACCTCCTGATCTATG14150.13188726481657884No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT13970.13020954696025486No Hit
GTGCAGCCACAGTTCGTTGAACCTCCAGTCGTGTCCCTTGGCCGAAGGTG13810.128718242199078No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13770.12834541600878377No Hit
GTTACAGTACCCCTCAGGTCACCTTCGGCCAAGGGACACGACTGGAGGTTCAACGAACTGTGGCTGCACCAT13710.12778617672334244No Hit
GTCTGCAACCTGCAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGT13440.1252695999388565No Hit
CGACTGGAGGTTCAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT13120.12228699041650277No Hit
GAGTGAAATCTGTCCCAGATCCATTGCCACTGAACCTTGATGGGACCCCA13070.121820957678635No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTGAACCTCCAGTCGTGTCC13070.121820957678635No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC12530.1167878041096631No Hit
GAGGGGTACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTGCAGGT12320.11483046661061846No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12190.11361878149216227No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC12090.11268671601642671No Hit
CATCAAGGTTCAGTGGCAATGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGCAGATTTTG12080.11259350946885317No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12000.11184785708826474No Hit
ATTTAAATTGGTATCAACAGAAGCCAGGGAAAGCCCCTAACCTCCTGATC11950.11138182435039698No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11940.11128861780282341No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA11870.11063617196980853No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11790.1098905195892201No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11740.10942448685135234No Hit
ATGCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAATGGATCTGGGACAGATTTCACTCTCA11710.10914486720863167No Hit
ACCTTGATGGGACCCCACTTTGCAAAGTGGATGCAGCATAGATCAGGAGG11680.10886524756591101No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11640.10849242137561678No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA11610.10821280173289613No Hit
GTACCCCTCAGGTCACCTTCGGCCAAGGGACACGACTGGAGGTTCAACGA11600.10811959518532259No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT11490.10709432316201349No Hit
CACCATCAGCAGTCTGCAACCTGCAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTACCCCTCAGGTCAC11480.10700111661443992No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11460.10681470351929281No Hit
GGTTAGGGGCTTTCCCTGGCTTCTGTTGATACCAATTTAAATAGGTGCCA11390.10616225768627795No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11160.1040185070920862No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC10890.10150193030760024No Hit
GTTCAGTGGCAATGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGCAGATTTTGCAACTTA10860.10122231066487959No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG10850.10112910411730602No Hit
CCTTCGGCCAAGGGACACGACTGGAGGTTCAACGAACTGTGGCTGCACCA10840.10103589756973248No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT703.9804218E-757.51901670
ACGTACG200.00832184550.16489420
CGTACGG352.7309248E-547.7783221
CGTATGC2450.042.3998441
CTTATAC3050.041.6786771
TCGTATG2600.041.1464640
TATACAC3350.039.9248123
ACGAGAC2950.039.68585222
TTATACA3450.038.767572
CCCTAAG3050.038.60609428
GCCGTCT2400.038.01300446
CACGAGA3100.037.7602821
CCCACGA3050.037.2775419
ATCTCGT2700.036.72699737
TAAGACA3250.036.40447631
ATGCCGT2900.036.10638444
ATCTCCG3150.036.08571610
GGTACGT701.3331788E-534.53402368
TCTCCGA3300.034.44545411
TACACAT3600.034.3658335
Produced by FastQC (version 0.11.5)