FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691778

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691778
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1072886
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT37500.34952455340082733No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37200.3467283569736207No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26000.24233702369124027No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25220.23506691298050306No Hit
GATCTATGCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAATGGATCTGGGACAGATTTCA25030.23329598857660555No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24850.23161827072028154No Hit
GAGTTACAGTACCCCTCAGGTCACCTTCGGCCAAGGGACACGACTGGAGGTTCAACGAACTGTGGCTGCACCAT24320.2266783236988832No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22590.2105535909686584No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG22540.2100875582307906No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22520.20990114513564348No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21540.20076690347343523No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGGATTGGCACCTATTTAAATTGGTATCAACAGAAG21430.1997416314501261No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGGATTGGCAC19540.1821255939587244No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTGCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC19380.18063428919754757No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC18950.17662640765188473No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18610.17345738503438388No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18350.1710340147974715No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18280.1703815689644566No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18240.1700087427741624No Hit
GCATAGATCAGGAGGTTAGGGGCTTTCCCTGGCTTCTGTTGATACCAATT17900.16683972015666157No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17500.16311145825371942No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17130.1596628159934979No Hit
GTACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTGCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT17000.15845113087504173No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT16930.15779868504202682No Hit
GTAGTAAGTTGCAAAATCTGCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCATTGCCA16820.15677341301871772No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16270.15164705290217226No Hit
GGACACGACTGGAGGTTCAACGAACTGTGGCTGCACCATCTGTCTTCATC16110.15015574814099542No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16090.1499693350458483No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG15990.14903726957011276No Hit
ACTTACTACTGTCAACAGAGTTACAGTACCCCTCAGGTCACCTTCGGCCA15250.14213998504966976No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15190.14158074576422844No Hit
GTCGTGTCCCTTGGCCGAAGGTGACCTGAGGGGTACTGTAACTCTGTTGA15140.14111471302636067No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15020.13999623445547804No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGCAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTACC14740.13738645112341852No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT14680.1368272118379772No Hit
GTGCAGCCACAGTTCGTTGAACCTCCAGTCGTGTCCCTTGGCCGAAGGTG14150.13188726481657884No Hit
AGTCAGAGGATTGGCACCTATTTAAATTGGTATCAACAGAAGCCAGGGAAAGCCCCTAACCTCCTGATCTATG13770.12834541600878377No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13650.12722693743790114No Hit
GTCTGCAACCTGCAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGT13580.12657449160488626No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT13530.1261084588670185No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13420.1250831868437094No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13400.12489677374856228No Hit
GATCAGGAGGTTAGGGGCTTTCCCTGGCTTCTGTTGATACCAATTTAAAT13330.1242443279155474No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13010.12126171839319368No Hit
GAGTGAAATCTGTCCCAGATCCATTGCCACTGAACCTTGATGGGACCCCA12910.12032965291745815No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAATGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTG12900.12023644636988459No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA12780.11911796779900195No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12780.11911796779900195No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12490.11641497791936888No Hit
GAGGGGTACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTGCAGGT12450.11604215172907467No Hit
GTTACAGTACCCCTCAGGTCACCTTCGGCCAAGGGACACGACTGGAGGTTCAACGAACTGTGGCTGCACCAT12440.1159489451815011No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12420.115762532086354No Hit
GGTTAGGGGCTTTCCCTGGCTTCTGTTGATACCAATTTAAATAGGTGCCA12290.1145508469678978No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTGAACCTCCAGTCGTGTCC12250.11417802077760357No Hit
ATGCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAATGGATCTGGGACAGATTTCACTCTCA12110.11287312911157382No Hit
CGACTGGAGGTTCAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT12110.11287312911157382No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC11930.11119541125524987No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11890.11082258506495564No Hit
CACCATCAGCAGTCTGCAACCTGCAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTACCCCTCAGGTCAC11790.1098905195892201No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11730.10933128030377878No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11650.10858562792319035No Hit
GTGTATTACTGTGCGAGAGATAGAAAGTGGCTGGTGGAAATTGACTACTGGGGCCAGGGAACTCTGGTCACCGT11380.1060690511387044No Hit
CATCAAGGTTCAGTGGCAATGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGCAGATTTTG11250.1048573660202482No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA11210.10448453982995398No Hit
ATTTAAATTGGTATCAACAGAAGCCAGGGAAAGCCCCTAACCTCCTGATC11080.10327285471149777No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10890.10150193030760024No Hit
CTCCTGATCTATGCTGCATCCACTTTGCAAAGTGGGGTCCCATCAAGGTT10820.10084948447458537No Hit
CTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCATTGCCACTGAACCT10770.10038345173671762No Hit
ACCTTGATGGGACCCCACTTTGCAAAGTGGATGCAGCATAGATCAGGAGG10770.10038345173671762No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACTCA200.006129549454.1977156
ACTCGCG200.006202061754.03682755
TAGATTG450.003121427733.22713564
GTACGGA450.00541023329.6955971
ATCAACG41750.028.7077393
TCAACGC42150.028.3587444
AACGCAG42050.028.2700146
CAACGCA42300.028.2608175
GGCACTC606.267611E-428.04637528
CGCAGAG42850.027.6642938
AGAGTAC43000.027.56393411
GCAGAGT43500.027.1716189
GACCGGA1308.630434E-426.54910370
TAAAGTC959.418551E-625.78169645
TACGTGA700.001066679825.60557453
TGTTAGA13900.024.83009770
CCACGTG3901.8189894E-1224.77916370
AGTACGG33750.024.74171413
GAGTACG33850.024.6640212
ACGATTA700.001327286524.66343740
Produced by FastQC (version 0.11.5)