FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004691779

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691779
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56112
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT33015.882877102936983No Hit
GTATCAACGCAGAGTACGGGTGACCACGGGTGACGGGGAATCAGGGTTCG8801.568291987453664No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG6011.0710721414314228No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCT5731.0211719418306244No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGA5510.9819646421442828No Hit
GAGTACGGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACAT5040.8982035928143712No Hit
GGTATCAACGCAGAGTACGGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAA3630.6469204448246365No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGA2980.5310806957513544No Hit
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT2910.5186056458511549TruSeq Adapter, Index 12 (95% over 22bp)
GAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATC2730.48652694610778446No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT2680.4776161961790705No Hit
CCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAA2640.4704875962360992No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCC2570.4580125463358996No Hit
GGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGG2480.44197319646421446No Hit
CGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCT2460.43840889649272885No Hit
GGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCA2350.41880524664955804No Hit
TATCAACGCAGAGTACGGGTGACCACGGGTGACGGGGAATCAGGGTTCGA2310.4116766467065869No Hit
TCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCG2180.38850869689193046No Hit
GGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTT2070.3689050470487596No Hit
ATCAACGCAGAGTACGGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGG2060.3671228970630168No Hit
GGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACC1950.347519247219846No Hit
GTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTT1800.32078699743370404No Hit
GTACGGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGG1790.3190048474479612No Hit
ACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCC1730.30831194753350444No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATGTCGTATGCCGT1720.3065297975477616No Hit
GGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACA1720.3065297975477616No Hit
CTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCT1600.285143997718848No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACC1580.2815796977473624No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1550.27623324779013403No Hit
CCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT1550.27623324779013403No Hit
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTCT1480.26375819788993443RNA PCR Primer, Index 12 (95% over 24bp)
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCCGTACTCTGCGTTGATAC1400.249500998003992No Hit
CGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCA1370.2441545480467636No Hit
CCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACC1320.2352437981180496No Hit
GTATCAACGCAGAGTACGGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAG1320.2352437981180496No Hit
CCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCC1220.2174222982606216No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATG1220.2174222982606216No Hit
GAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGC1210.21564014827487882No Hit
CACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGG1210.21564014827487882No Hit
ACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCG1120.19960079840319359No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1070.19069004847447962No Hit
TCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACC1060.1889078984887368No Hit
GTATCAACGCAGAGTACGGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAA1060.1889078984887368No Hit
CGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACG1010.1799971485600228No Hit
GCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGT1010.1799971485600228No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT960.1710863986313088No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT920.16395779868833762No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT920.16395779868833762No Hit
GGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAG910.1621756487025948No Hit
CCCTGATTCCCCGTCACCCGTGGTCACCCGTACTCTGCGTTGATAC890.15861134873110921No Hit
GTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACT870.1550470487596236No Hit
CCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCA850.151482748788138No Hit
ATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTA850.151482748788138No Hit
GTATCAACGCAGAGTACGGGTGACCACGGGTGACGGGGAATCAGGG840.14970059880239522No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT820.14613629883090962No Hit
CCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGG810.1443541488451668No Hit
ATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGAG800.142571998859424No Hit
CGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCG760.1354433989164528No Hit
GGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCCGTA750.13366124893071002No Hit
GAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGA730.1300969489592244No Hit
GGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCG730.1300969489592244No Hit
TCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTC690.12296834901625321No Hit
GTGGTATCAACGCAGAGTACGGGTGACCACGGGTGACGGGGAATCAGGGT690.12296834901625321No Hit
TGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCG640.11405759908753922No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT640.11405759908753922No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATG630.1122754491017964No Hit
AAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGAGGTAG620.11049329911605361No Hit
GGTATCAACGCAGAGTACGGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAG610.1087111491303108No Hit
TTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC590.10514684915882522No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCC570.10158254918733961No Hit
GTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACC570.10158254918733961No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATATCGTATGCCGT570.10158254918733961No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG4350.0130.3294570
CGTTTAA100.009901646129.4198869
AAGGGGG4500.084.8419269
AAAGGGG4400.080.25831668
AAAAGGG4450.077.46709467
AAAAAGG4400.075.71271566
AAAAAAG4800.068.19630465
TGAAAAA6350.064.2506659
CTTGAAA6050.063.493557
GCTTGAA6050.062.84749256
TTGAAAA6350.061.6710858
TGCTTGA5900.061.1980555
ATGGGGG652.9471266E-759.73225469
CTGCTTG5900.058.558154
TCTGCTT5900.058.013153
GTCGTAT308.891375E-657.60563339
ACGGGTA150.00502534556.91703816
TGGGGGG859.671163E-656.52374370
TTCTGCT5950.056.5150152
TGTCGTA301.0162821E-556.3241538
Produced by FastQC (version 0.11.5)