FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004691780

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004691780
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56112
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG54719.75014257199886No Hit
GTATCAACGCAGAGTACGGGTGACCACGGGTGACGGGGAATCAGGGTTCG8691.5486883376104934No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG6761.204733390362133No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCT6361.133447390932421No Hit
GAGTACGGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACAT4640.8269175933846592No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGA4490.8001853435985173No Hit
GGTATCAACGCAGAGTACGGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAA3590.6397918448816652No Hit
GAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATC2950.5257342457941261No Hit
CGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCT2460.43840889649272885No Hit
GGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCA2430.43306244653550047No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT2330.41524094667807243No Hit
GGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGG2190.39029084687767324No Hit
GGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTT2170.38672654690618763No Hit
GTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTT2150.383162246934702No Hit
CTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCT2070.3689050470487596No Hit
TATCAACGCAGAGTACGGGTGACCACGGGTGACGGGGAATCAGGGTTCGA2030.36177644710578843No Hit
GGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACC2000.35642999714856005No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGA1840.32791559737667525No Hit
CCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAA1780.31722269746221843No Hit
CCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT1690.3011833475905332No Hit
CCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACC1590.28336184773310524No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATG1520.2708867978329056No Hit
ACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCC1490.2655403478756772No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1430.25484744796122044No Hit
CCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCC1410.25128314798973483No Hit
CGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCA1380.24593669803250642No Hit
GTATCAACGCAGAGTACGGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAG1360.24237239806102082No Hit
GTACGGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGG1330.23702594810379243No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCCGTACTCTGCGTTGATAC1320.2352437981180496No Hit
ATCAACGCAGAGTACGGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGG1300.23167949814656402No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1280.22811519817507844No Hit
GGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACA1270.22633304818933558No Hit
GAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGC1240.22098659823210723No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACC1220.2174222982606216No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1190.2120758483033932No Hit
CACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGG1170.2085115483319076No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAAAAAAAAAAAA1110.1978186484174508No Hit
GTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACT1100.196036498431708No Hit
GTATCAACGCAGAGTACGGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAA1090.19425434844596523No Hit
GGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAG1050.18712574850299402No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1020.1817792985457656No Hit
CGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACG1000.17821499857428003No Hit
CCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGG990.17643284858853722No Hit
TCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACC950.169304248645566No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG900.16039349871685202No Hit
GTATCAACGCAGAGTACGGGTGACCACGGGTGACGGGGAATCAGGG890.15861134873110921No Hit
TCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTC890.15861134873110921No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT880.1568291987453664No Hit
GTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACC860.1532648987738808No Hit
ACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCG860.1532648987738808No Hit
GTGGTATCAACGCAGAGTACGGGTGACCACGGGTGACGGGGAATCAGGGT840.14970059880239522No Hit
CCCTGATTCCCCGTCACCCGTGGTCACCCGTACTCTGCGTTGATAC830.1479184488166524No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG820.14613629883090962No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG760.1354433989164528No Hit
GCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGT740.13187909894496722No Hit
ATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGAG710.1265326489877388No Hit
GCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAAT690.12296834901625321No Hit
CGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCG680.12118619903051041No Hit
GGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCCGTA680.12118619903051041No Hit
CCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACCC660.1176218990590248No Hit
GGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCG650.11583974907328201No Hit
TCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCT640.11405759908753922No Hit
TGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCG630.1122754491017964No Hit
ATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTA620.11049329911605361No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGAGGTAG600.10692899914456801No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT600.10692899914456801No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCCGTACTCTGCGTTGATACC600.10692899914456801No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT590.10514684915882522No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT570.10158254918733961No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT570.10158254918733961No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAAT570.10158254918733961No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)